Late assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7pub
Q-score: 0.436
Lenarcic T, Niemann M, Ramrath DJF, Calderaro S, Flugel T, Saurer M, Leibundgut M, Boehringer D, Prange C, Horn EK, Schneider A, Ban N
PNAS (2022) 119 [ Pubmed: 35042777 DOI: doi:10.1073/pnas.2114710118 ]
- Ms69 (21 kDa, Protein from Trypanosoma brucei brucei)
- Ms71 (20 kDa, Protein from Trypanosoma brucei brucei)
- Sod_fe_c domain-containing protein (48 kDa, Protein from Trypanosoma brucei brucei)
- Bs21m (23 kDa, Protein from Trypanosoma brucei brucei)
- Ms50 (131 kDa, Protein from Trypanosoma brucei brucei)
- Ms52 (84 kDa, Protein from Trypanosoma brucei brucei)
- Unkk (273 Da, Protein from Trypanosoma brucei brucei)
- Mt-saf3 (106 kDa, Protein from Trypanosoma brucei brucei)
- Us9m (50 kDa, Protein from Trypanosoma brucei brucei)
- Ms49 (137 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf38 (69 kDa, Protein from Trypanosoma brucei brucei)
- Ms38 (28 kDa, Protein from Trypanosoma brucei brucei)
- Ms63 (31 kDa, Protein from Trypanosoma brucei brucei)
- Ms72 (19 kDa, Protein from Trypanosoma brucei brucei)
- Us19m (28 kDa, Protein from Trypanosoma brucei brucei)
- Magnesium ion (24 Da, Ligand)
- Ubiquitin-like domain-containing protein (26 kDa, Protein from Trypanosoma brucei brucei)
- Bs16m (22 kDa, Protein from Trypanosoma brucei brucei)
- Ms59 (35 kDa, Protein from Trypanosoma brucei brucei)
- Unki (869 Da, Protein from Trypanosoma brucei brucei)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Ribosomal_s5_c domain-containing protein (50 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf37 (37 kDa, Protein from Trypanosoma brucei brucei)
- Ms58 (35 kDa, Protein from Trypanosoma brucei brucei)
- Unk7 (3 kDa, Protein from Trypanosoma brucei brucei)
- Ms53 (75 kDa, Protein from Trypanosoma brucei brucei)
- Bs6m (18 kDa, Protein from Trypanosoma brucei brucei)
- Ms51 (95 kDa, Protein from Trypanosoma brucei brucei)
- Bs18m (36 kDa, Protein from Trypanosoma brucei brucei)
- Unkl (1 kDa, Protein from Trypanosoma brucei brucei)
- 30s ribosomal protein s8, putative (32 kDa, Protein from Trypanosoma brucei brucei)
- Ms37 (24 kDa, Protein from Trypanosoma brucei brucei)
- Us12m (10 kDa, Protein from Trypanosoma brucei brucei)
- 9s rrna (198 kDa, RNA from Trypanosoma brucei brucei)
- 30s ribosomal protein s17, putative (35 kDa, Protein from Trypanosoma brucei brucei)
- Akap7_nls domain-containing protein (30 kDa, Protein from Trypanosoma brucei brucei)
- Us14m (19 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf7 (76 kDa, Protein from Trypanosoma brucei brucei)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Unkf (3 kDa, Protein from Trypanosoma brucei brucei)
- Ms65 (30 kDa, Protein from Trypanosoma brucei brucei)
- Us11m (37 kDa, Protein from Trypanosoma brucei brucei)
- Uridine 5'-triphosphate (484 Da, Ligand)
- Unkg (1 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf39 (89 kDa, Protein from Trypanosoma brucei brucei)
- Phosphate ion (94 Da, Ligand)
- Lysm domain-containing protein (94 kDa, Protein from Trypanosoma brucei brucei)
- Unke (4 kDa, Protein from Trypanosoma brucei brucei)
- Ms34 (28 kDa, Protein from Trypanosoma brucei brucei)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Water (18 Da, Ligand)
- Ms48 (201 kDa, Protein from Trypanosoma brucei brucei)
- Zinc ion (65 Da, Ligand)
- Ms70 (21 kDa, Protein from Trypanosoma brucei brucei)
- Ms56 (47 kDa, Protein from Trypanosoma brucei brucei)
- Unk (1 kDa, Protein from Trypanosoma brucei brucei)
- Late assembly intermediate of the trypanosoma brucei mitoribosomal small subunit (Complex from Trypanosoma brucei brucei)
- Ms35 (98 kDa, Protein from Trypanosoma brucei brucei)
- Acyl transferase-like protein (60 kDa, Protein from Trypanosoma brucei brucei)
- Dna photolyase, putative (95 kDa, Protein from Trypanosoma brucei brucei)
- Ms55 (66 kDa, Protein from Trypanosoma brucei brucei)
- Translation initiation factor if-2, putative (87 kDa, Protein from Trypanosoma brucei brucei)
- Ms61 (33 kDa, Protein from Trypanosoma brucei brucei)
- Ms73 (11 kDa, Protein from Trypanosoma brucei brucei)
- Us3m (9 kDa, Protein from Trypanosoma brucei brucei)
- Ms22 (73 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf6 (76 kDa, Protein from Trypanosoma brucei brucei)
- Superoxide dismutase, putative (29 kDa, Protein from Trypanosoma brucei brucei)
- Ms57 (45 kDa, Protein from Trypanosoma brucei brucei)
- Ms33 (21 kDa, Protein from Trypanosoma brucei brucei)
- Protein fyv4, mitochondrial (22 kDa, Protein from Trypanosoma brucei brucei)
- Rhodanese domain-containing protein (30 kDa, Protein from Trypanosoma brucei brucei)
- Ms54 (72 kDa, Protein from Trypanosoma brucei brucei)
- Ms60 (34 kDa, Protein from Trypanosoma brucei brucei)
- Ms29 (57 kDa, Protein from Trypanosoma brucei brucei)
- Ms62 (32 kDa, Protein from Trypanosoma brucei brucei)
- Ms26 (51 kDa, Protein from Trypanosoma brucei brucei)
- Ms74 (8 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf22 (39 kDa, Protein from Trypanosoma brucei brucei)
- Us15m (50 kDa, Protein from Trypanosoma brucei brucei)
- Ms23 (36 kDa, Protein from Trypanosoma brucei brucei)
- Ms66 (29 kDa, Protein from Trypanosoma brucei brucei)
- Ech_2 domain-containing protein (137 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf9 (69 kDa, Protein from Trypanosoma brucei brucei)
Trypanosoma brucei mitochondrial ribosome large subunit assembly intermediate
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7aoi
Q-score: 0.486
Tobiasson V, Gahura O, Aibara S, Baradaran R, Zikova A, Amunts A
EMBO J (2021) 40 pp. e106292-e106292 [ DOI: doi:10.15252/embj.2020106292 Pubmed: 33576519 ]
- Ml74 (25 kDa, Protein from Trypanosoma brucei)
- Mt-laf7,mt-laf7 (18 kDa, Protein from Trypanosoma brucei)
- Ml41 (13 kDa, Protein from Trypanosoma brucei)
- Ribosomal protein l11,ul11m (29 kDa, Protein from Trypanosoma brucei)
- Mt-laf4 (69 kDa, Protein from Trypanosoma brucei)
- Ml94 (11 kDa, Protein from Trypanosoma brucei)
- Ml76 (18 kDa, Protein from Trypanosoma brucei)
- Ml53 (33 kDa, Protein from Trypanosoma brucei)
- Ribosomal_l18e/l15p domain-containing protein (41 kDa, Protein from Trypanosoma brucei)
- Unk10 (1 kDa, Protein from Trypanosoma brucei)
- Mt-laf14 (78 kDa, Protein from Trypanosoma brucei)
- Peptidyl-prolyl cis-trans isomerase (20 kDa, Protein from Trypanosoma brucei)
- Mt-laf15_1 (21 kDa, Protein from Trypanosoma brucei)
- Ml70 (47 kDa, Protein from Trypanosoma brucei)
- Mt-laf8 (58 kDa, Protein from Trypanosoma brucei)
- Mitochondrial ribosomal protein l51 / s25 / ci-b8 domain containing protein (22 kDa, Protein from Trypanosoma brucei)
- Chaperone protein dnaj, putative,ml77,chaperone protein dnaj, putative (30 kDa, Protein from Trypanosoma brucei)
- Tetratricopeptide repeat (48 kDa, Protein from Trypanosoma brucei)
- Bl19m (16 kDa, Protein from Trypanosoma brucei)
- Ml85 (16 kDa, Protein from Trypanosoma brucei)
- Ribosomal protein l22p/l17e (31 kDa, Protein from Trypanosoma brucei)
- Ml67 (89 kDa, Protein from Trypanosoma brucei)
- Rna uridylyltransferase (17 kDa, Protein from Trypanosoma brucei)
- Unk6/mt-laf15_2 (9 kDa, Protein from Trypanosoma brucei)
- Unk3 (1 kDa, Protein from Trypanosoma brucei)
- Ul29m (53 kDa, Protein from Trypanosoma brucei)
- Acyl carrier protein (9 kDa, Protein from Trypanosoma brucei)
- Zinc ion (65 Da, Ligand)
- Ml63 (17 kDa, Protein from Trypanosoma brucei)
- Bl32m (6 kDa, Protein from Trypanosoma brucei)
- Magnesium ion (24 Da, Ligand)
- Gtp-binding protein, putative,mt-enga (60 kDa, Protein from Trypanosoma brucei)
- Ml95 (16 kDa, Protein from Trypanosoma brucei)
- Peptidyl-prolyl cis-trans isomerase (20 kDa, Protein from Trypanosoma brucei)
- Unk1 (3 kDa, Protein from Trypanosoma brucei)
- Ribosomal protein l21 (20 kDa, Protein from Trypanosoma brucei)
- Bl28m (25 kDa, Protein from Trypanosoma brucei)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ml49 (20 kDa, Protein from Trypanosoma brucei)
- Ml78 (31 kDa, Protein from Trypanosoma brucei)
- Ml101 (21 kDa, Protein from Trypanosoma brucei)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Ul24m (39 kDa, Protein from Trypanosoma brucei)
- Unk7 (1 kDa, Protein from Trypanosoma brucei)
- Spou rrna methylase family (48 kDa, Protein from Trypanosoma brucei)
- Ribosomal protein l4/l1 family (51 kDa, Protein from Trypanosoma brucei)
- Bl20m (22 kDa, Protein from Trypanosoma brucei)
- Ml99 (9 kDa, Protein from Trypanosoma brucei)
- 50s ribosomal protein l14 (19 kDa, Protein from Trypanosoma brucei)
- Ml52 (14 kDa, Protein from Trypanosoma brucei)
- Unk8 (1 kDa, Protein from Trypanosoma brucei)
- Ribosomal protein l23 (19 kDa, Protein from Trypanosoma brucei)
- Unk9 (2 kDa, Protein from Trypanosoma brucei)
- Ml93 (16 kDa, Protein from Trypanosoma brucei)
- Pseudouridylate synthase, putative (42 kDa, Protein from Trypanosoma brucei)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Ml87 (9 kDa, Protein from Trypanosoma brucei)
- Gtp-binding protein (33 kDa, Protein from Trypanosoma brucei)
- Ul30m (17 kDa, Protein from Trypanosoma brucei)
- Unk5 (870 Da, Protein from Trypanosoma brucei)
- Complex1-lyr-like (10 kDa, Protein from Trypanosoma brucei)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Bl33m (17 kDa, Protein from Trypanosoma brucei)
- Mt-laf12 (70 kDa, Protein from Trypanosoma brucei)
- Ml100,ml100 (10 kDa, Protein from Trypanosoma brucei)
- Ml84 (23 kDa, Protein from Trypanosoma brucei)
- Mt-lsu rrna (242 kDa, RNA from Trypanosoma brucei)
- Dead-box helicase, putative,mt-laf2 (76 kDa, Protein from Trypanosoma brucei)
- Lipase (class 3) (18 kDa, Protein from Trypanosoma brucei)
- Unk4 (586 Da, Protein from Trypanosoma brucei)
- Ml89 (22 kDa, Protein from Trypanosoma brucei)
- Trypanosoma brucei mitochondrial ribosome large subunit assembly intermediate (2 MDa, Complex from Trypanosoma brucei)
- Mitochondrial rna binding complex 1 subunit (37 kDa, Protein from Trypanosoma brucei)
- Ml80 (25 kDa, Protein from Trypanosoma brucei)
- Ribosomal_l9 domain-containing protein (25 kDa, Protein from Trypanosoma brucei)
- Mt-laf19 (11 kDa, Protein from Trypanosoma brucei)
- Ml86 (19 kDa, Protein from Trypanosoma brucei)
- Lim domain containing protein,ml90 (13 kDa, Protein from Trypanosoma brucei)
- Ml71 (49 kDa, Protein from Trypanosoma brucei)
- Ml64,ml64 (23 kDa, Protein from Trypanosoma brucei)
- Ml42 (15 kDa, Protein from Trypanosoma brucei)
- Ml98 (10 kDa, Protein from Trypanosoma brucei)
- Unk2 (657 Da, Protein from Trypanosoma brucei)
- Ribosomal protein l3 mitochondrial, putative (42 kDa, Protein from Trypanosoma brucei)
- 50s ribosomal protein l17 (30 kDa, Protein from Trypanosoma brucei)
- Unk11 (3 kDa, Protein from Trypanosoma brucei)
- Ml68,ml68,ml68 (40 kDa, Protein from Trypanosoma brucei)
- 50s ribosomal protein l13 (20 kDa, Protein from Trypanosoma brucei)
- Ml81 (25 kDa, Protein from Trypanosoma brucei)