Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state A
Resolution: 7.0 Å
EM Method: Single-particle
Fitted PDBs: 6rxt
Q-score: 0.166
Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E
Mol Cell (2019) 75 pp. 1256-1269.e7 [ DOI: doi:10.1016/j.molcel.2019.06.032 Pubmed: 31378463 ]
- Utp24 (22 kDa, Protein from Chaetomium thermophilum)
- Rrp9 (69 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s23-like protein (15 kDa, Protein from Chaetomium thermophilum)
- Putative nucleolar protein (57 kDa, Protein from Chaetomium thermophilum)
- Utp17 (105 kDa, Protein from Chaetomium thermophilum)
- Kre33 (119 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s7 (23 kDa, Protein from Chaetomium thermophilum)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Imp3 (21 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s14-like protein (16 kDa, Protein from Chaetomium thermophilum)
- Putative u3 small nucleolar ribonucleoprotein protein (86 kDa, Protein from Chaetomium thermophilum)
- U3 snorna (73 kDa, RNA from Chaetomium thermophilum)
- Bms1 (134 kDa, Protein from Chaetomium thermophilum)
- Faf1 (34 kDa, Protein from Chaetomium thermophilum)
- Putative ribosomal protein (17 kDa, Protein from Chaetomium thermophilum)
- Utp4 (97 kDa, Protein from Chaetomium thermophilum)
- Enp1 (54 kDa, Protein from Chaetomium thermophilum)
- Utp2 (103 kDa, Protein from Chaetomium thermophilum)
- Utp3 (73 kDa, Protein from Chaetomium thermophilum)
- Zinc ion (65 Da, Ligand)
- Utp13" (100 kDa, Protein from Chaetomium thermophilum)
- Pre-rrna-processing protein pno1 (28 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s1 (29 kDa, Protein from Chaetomium thermophilum)
- Putative u3 small nucleolar ribonucleoprotein (34 kDa, Protein from Chaetomium thermophilum)
- Noc4 (63 kDa, Protein from Chaetomium thermophilum)
- Rna 3'-terminal phosphate cyclase-like protein (43 kDa, Protein from Chaetomium thermophilum)
- Nop58 (64 kDa, Protein from Chaetomium thermophilum)
- Krr1 small subunit processome component (37 kDa, Protein from Chaetomium thermophilum)
- Utp8 (102 kDa, Protein from Chaetomium thermophilum)
- Utp16 (40 kDa, Protein from Chaetomium thermophilum)
- 90s pre-ribosome, state a (Complex from Chaetomium thermophilum)
- 40s ribosomal protein s28-like protein (7 kDa, Protein from Chaetomium thermophilum)
- Utp14 (104 kDa, Protein from Chaetomium thermophilum)
- Nop1 (32 kDa, Protein from Chaetomium thermophilum)
- U3 small nucleolar rna-associated protein 11 (31 kDa, Protein from Chaetomium thermophilum)
- Utp6 (46 kDa, Protein from Chaetomium thermophilum)
- Utp18 (69 kDa, Protein from Chaetomium thermophilum)
- Utp7 (63 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s5-like protein (23 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s13-like protein (16 kDa, Protein from Chaetomium thermophilum)
- Utp5" (44 kDa, Protein from Chaetomium thermophilum)
- Periodic tryptophan protein 2-like protein (100 kDa, Protein from Chaetomium thermophilum)
- Utp10 (198 kDa, Protein from Chaetomium thermophilum)
- Fcf2 (22 kDa, Protein from Chaetomium thermophilum)
- Putative u3 snornp protein (115 kDa, Protein from Chaetomium thermophilum)
- Utp12 (107 kDa, Protein from Chaetomium thermophilum)
- Utp15 (60 kDa, Protein from Chaetomium thermophilum)
- Sof1 (50 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s22-like protein (14 kDa, Protein from Chaetomium thermophilum)
- 35s ribosomal rna (758 kDa, RNA from Chaetomium thermophilum)
- Magnesium ion (24 Da, Ligand)
- Emg1 (27 kDa, Protein from Chaetomium thermophilum)
- Snu13 (13 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s9-like protein (22 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s16-like protein (15 kDa, Protein from Chaetomium thermophilum)
- Utp30 (43 kDa, Protein from Chaetomium thermophilum)
Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state B1
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6rxu
Q-score: 0.44
Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E
Mol Cell (2019) 75 pp. 1256-1269.e7 [ DOI: doi:10.1016/j.molcel.2019.06.032 Pubmed: 31378463 ]
- Utp24 (22 kDa, Protein from Chaetomium thermophilum)
- Utp13 (100 kDa, Protein from Chaetomium thermophilum)
- 90s pre-ribosome, state b1 (Complex from Chaetomium thermophilum)
- Rrp9 (69 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s23-like protein (15 kDa, Protein from Chaetomium thermophilum)
- Utp17 (105 kDa, Protein from Chaetomium thermophilum)
- Kre33 (119 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s7 (23 kDa, Protein from Chaetomium thermophilum)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Imp3 (21 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s14-like protein (16 kDa, Protein from Chaetomium thermophilum)
- Putative u3 small nucleolar ribonucleoprotein protein (86 kDa, Protein from Chaetomium thermophilum)
- U3 snorna (73 kDa, RNA from Chaetomium thermophilum)
- Bms1 (134 kDa, Protein from Chaetomium thermophilum)
- Faf1 (34 kDa, Protein from Chaetomium thermophilum)
- Putative ribosomal protein (17 kDa, Protein from Chaetomium thermophilum)
- Utp4 (97 kDa, Protein from Chaetomium thermophilum)
- Enp1 (54 kDa, Protein from Chaetomium thermophilum)
- Utp2 (103 kDa, Protein from Chaetomium thermophilum)
- Utp3 (73 kDa, Protein from Chaetomium thermophilum)
- Zinc ion (65 Da, Ligand)
- 35s rrna (758 kDa, RNA from Chaetomium thermophilum)
- Pre-rrna-processing protein pno1 (28 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s1 (29 kDa, Protein from Chaetomium thermophilum)
- Putative u3 small nucleolar ribonucleoprotein (34 kDa, Protein from Chaetomium thermophilum)
- Noc4 (63 kDa, Protein from Chaetomium thermophilum)
- Rna 3'-terminal phosphate cyclase-like protein (43 kDa, Protein from Chaetomium thermophilum)
- Nop58 (64 kDa, Protein from Chaetomium thermophilum)
- Krr1 small subunit processome component (37 kDa, Protein from Chaetomium thermophilum)
- Utp8 (102 kDa, Protein from Chaetomium thermophilum)
- Bfr2 (69 kDa, Protein from Chaetomium thermophilum)
- Utp16 (40 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s28-like protein (7 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s11-like protein (18 kDa, Protein from Chaetomium thermophilum)
- Utp14 (104 kDa, Protein from Chaetomium thermophilum)
- Nop1 (32 kDa, Protein from Chaetomium thermophilum)
- Nop56 (57 kDa, Protein from Chaetomium thermophilum)
- Snu13 (13 kDa, Protein from Chaetomium thermophilum)
- U3 small nucleolar rna-associated protein 11 (31 kDa, Protein from Chaetomium thermophilum)
- Utp6 (46 kDa, Protein from Chaetomium thermophilum)
- Utp18 (69 kDa, Protein from Chaetomium thermophilum)
- Utp7 (63 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s5-like protein (23 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s13-like protein (16 kDa, Protein from Chaetomium thermophilum)
- Utp5 (44 kDa, Protein from Chaetomium thermophilum)
- Ribosome biogenesis protein enp2 (75 kDa, Protein from Chaetomium thermophilum)
- Periodic tryptophan protein 2-like protein (100 kDa, Protein from Chaetomium thermophilum)
- Utp10 (198 kDa, Protein from Chaetomium thermophilum)
- Fcf2 (22 kDa, Protein from Chaetomium thermophilum)
- Putative u3 snornp protein (115 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s4 (29 kDa, Protein from Chaetomium thermophilum)
- Utp12 (107 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s8 (23 kDa, Protein from Chaetomium thermophilum)
- Utp15 (60 kDa, Protein from Chaetomium thermophilum)
- Sof1 (50 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s22-like protein (14 kDa, Protein from Chaetomium thermophilum)
- U3 small nucleolar ribonucleoprotein protein lcp5-like protein (42 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s6 (27 kDa, Protein from Chaetomium thermophilum)
- Magnesium ion (24 Da, Ligand)
- Emg1 (27 kDa, Protein from Chaetomium thermophilum)
- Utp20 (295 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s9-like protein (22 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s24 (15 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s16-like protein (15 kDa, Protein from Chaetomium thermophilum)
- Utp30 (43 kDa, Protein from Chaetomium thermophilum)
Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state B2
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6rxv
Q-score: 0.371
Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E
Mol Cell (2019) 75 pp. 1256-1269.e7 [ DOI: doi:10.1016/j.molcel.2019.06.032 Pubmed: 31378463 ]
- Utp10 (198 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s5-like protein (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nop56 (57 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- U3 small nucleolar rna-associated protein 22 (131 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nop1 (32 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp2 (103 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s13-like protein (16 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp8 (102 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Imp4 (34 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Zinc ion (65 Da, Ligand)
- Utp18 (69 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Periodic tryptophan protein 2-like protein (100 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s8 (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp15 (60 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Kre33 (119 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rrp9 (69 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Sof1 (50 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Noc4 (63 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp13 (100 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Enp1 (54 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s16-like protein (15 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- U3 small nucleolar rna-associated protein 11 (31 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s9-like protein (22 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s28-like protein (7 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- U3 small nucleolar ribonucleoprotein protein lcp5-like protein (42 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp24 (22 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp20 (295 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Pre-rrna-processing protein pno1 (28 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Fcf2 (22 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp21 (115 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s6 (27 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s11-like protein (18 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- U3 snorna (73 kDa, RNA from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Utp3 (73 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Snu13 (13 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp14 (104 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s7 (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Krr1 small subunit processome component (37 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp7 (63 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp30 (43 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 90s pre-ribosome, state b2 (Complex from Chaetomium thermophilum)
- Imp3 (21 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rrp7 (36 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp17 (105 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Emg1 (27 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp16 (40 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp6 (46 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp4 (97 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s4 (29 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Bms1 (134 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Mpp10 (86 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s1 (29 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 35s ribosomal rna (758 kDa, RNA from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Faf1 (34 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rna 3'-terminal phosphate cyclase-like protein (43 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rps18 (17 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Magnesium ion (24 Da, Ligand)
- Utp12 (107 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s22-like protein (14 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Ribosome biogenesis protein enp2 (75 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp5 (44 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nop58 (64 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Bfr2 (69 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s23-like protein (15 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s24 (15 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s14-like protein (16 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state C
Resolution: 7.1 Å
EM Method: Single-particle
Fitted PDBs: 6rxx
Q-score: 0.156
Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E
Mol Cell (2019) 75 pp. 1256-1269.e7 [ DOI: doi:10.1016/j.molcel.2019.06.032 Pubmed: 31378463 ]
- Utp24 (22 kDa, Protein from Chaetomium thermophilum)
- 90s pre-ribosome, state c (Complex from Chaetomium thermophilum)
- Utp13 (100 kDa, Protein from Chaetomium thermophilum)
- Rrp9 (69 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s23-like protein (15 kDa, Protein from Chaetomium thermophilum)
- Putative nucleolar protein (57 kDa, Protein from Chaetomium thermophilum)
- Kre33 (119 kDa, Protein from Chaetomium thermophilum)
- Utp17 (105 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s7 (23 kDa, Protein from Chaetomium thermophilum)
- U3 small nucleolar rna-associated protein 22 (131 kDa, Protein from Chaetomium thermophilum)
- Imp3 (21 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s14-like protein (16 kDa, Protein from Chaetomium thermophilum)
- Putative u3 small nucleolar ribonucleoprotein protein (86 kDa, Protein from Chaetomium thermophilum)
- Putative ribosomal protein (17 kDa, Protein from Chaetomium thermophilum)
- Bms1 (134 kDa, Protein from Chaetomium thermophilum)
- Faf1 (34 kDa, Protein from Chaetomium thermophilum)
- Utp4 (97 kDa, Protein from Chaetomium thermophilum)
- Enp1 (54 kDa, Protein from Chaetomium thermophilum)
- Utp2 (103 kDa, Protein from Chaetomium thermophilum)
- Utp3 (73 kDa, Protein from Chaetomium thermophilum)
- Zinc ion (65 Da, Ligand)
- Pre-rrna-processing protein pno1 (28 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s1 (29 kDa, Protein from Chaetomium thermophilum)
- Putative u3 small nucleolar ribonucleoprotein (34 kDa, Protein from Chaetomium thermophilum)
- Noc4 (63 kDa, Protein from Chaetomium thermophilum)
- Rna 3'-terminal phosphate cyclase-like protein (43 kDa, Protein from Chaetomium thermophilum)
- Krr1 small subunit processome component (37 kDa, Protein from Chaetomium thermophilum)
- Rrp7 (36 kDa, Protein from Chaetomium thermophilum)
- Utp8 (102 kDa, Protein from Chaetomium thermophilum)
- Nop58 (64 kDa, Protein from Chaetomium thermophilum)
- Bfr2 (69 kDa, Protein from Chaetomium thermophilum)
- Ribosomal protein s27-like protein (8 kDa, Protein from Chaetomium thermophilum)
- Utp16 (40 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s28-like protein (7 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s11-like protein (18 kDa, Protein from Chaetomium thermophilum)
- U3 snorna (74 kDa, RNA from Chaetomium thermophilum)
- Utp14 (104 kDa, Protein from Chaetomium thermophilum)
- Nop1 (32 kDa, Protein from Chaetomium thermophilum)
- Snu13 (13 kDa, Protein from Chaetomium thermophilum)
- U3 small nucleolar rna-associated protein 11 (31 kDa, Protein from Chaetomium thermophilum)
- Utp6 (46 kDa, Protein from Chaetomium thermophilum)
- Utp18 (69 kDa, Protein from Chaetomium thermophilum)
- Utp7 (63 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s5-like protein (23 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s13-like protein (16 kDa, Protein from Chaetomium thermophilum)
- Utp5 (44 kDa, Protein from Chaetomium thermophilum)
- Ribosome biogenesis protein enp2 (75 kDa, Protein from Chaetomium thermophilum)
- Periodic tryptophan protein 2-like protein (100 kDa, Protein from Chaetomium thermophilum)
- Utp10 (198 kDa, Protein from Chaetomium thermophilum)
- 35s ribosomal rna (599 kDa, RNA from Chaetomium thermophilum)
- Fcf2 (22 kDa, Protein from Chaetomium thermophilum)
- Putative u3 snornp protein (115 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s4 (29 kDa, Protein from Chaetomium thermophilum)
- Utp12 (107 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s8 (23 kDa, Protein from Chaetomium thermophilum)
- Utp15 (60 kDa, Protein from Chaetomium thermophilum)
- Sof1 (50 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s22-like protein (14 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s6 (27 kDa, Protein from Chaetomium thermophilum)
- Emg1 (27 kDa, Protein from Chaetomium thermophilum)
- Utp20 (295 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s9-like protein (22 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s24 (15 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s19-like protein (17 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s16-like protein (15 kDa, Protein from Chaetomium thermophilum)
Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state a
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 6rxy
Q-score: 0.286
Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E
Mol Cell (2019) 75 pp. 1256-1269.e7 [ DOI: doi:10.1016/j.molcel.2019.06.032 Pubmed: 31378463 ]
- Utp24 (22 kDa, Protein from Chaetomium thermophilum)
- Utp13 (100 kDa, Protein from Chaetomium thermophilum)
- Rrp9 (69 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s23-like protein (15 kDa, Protein from Chaetomium thermophilum)
- Utp17 (105 kDa, Protein from Chaetomium thermophilum)
- Kre33 (119 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s7 (23 kDa, Protein from Chaetomium thermophilum)
- U3 small nucleolar rna-associated protein 22 (131 kDa, Protein from Chaetomium thermophilum)
- Utp10 (198 kDa, Protein from Chaetomium thermophilum)
- Imp3 (21 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s14-like protein (16 kDa, Protein from Chaetomium thermophilum)
- 35s rrna (749 kDa, RNA from Chaetomium thermophilum)
- Bms1 (134 kDa, Protein from Chaetomium thermophilum)
- Faf1 (34 kDa, Protein from Chaetomium thermophilum)
- U3 snorna (73 kDa, RNA from Chaetomium thermophilum)
- Utp4 (97 kDa, Protein from Chaetomium thermophilum)
- Enp1 (54 kDa, Protein from Chaetomium thermophilum)
- Utp2 (103 kDa, Protein from Chaetomium thermophilum)
- Utp3 (73 kDa, Protein from Chaetomium thermophilum)
- Zinc ion (65 Da, Ligand)
- Rrp5 (197 kDa, Protein from Chaetomium thermophilum)
- Pre-rrna-processing protein pno1 (28 kDa, Protein from Chaetomium thermophilum)
- Noc4 (63 kDa, Protein from Chaetomium thermophilum)
- Rna 3'-terminal phosphate cyclase-like protein (43 kDa, Protein from Chaetomium thermophilum)
- Nop58 (64 kDa, Protein from Chaetomium thermophilum)
- Krr1 small subunit processome component (37 kDa, Protein from Chaetomium thermophilum)
- Rrp7 (36 kDa, Protein from Chaetomium thermophilum)
- Utp8 (102 kDa, Protein from Chaetomium thermophilum)
- Utp16 (40 kDa, Protein from Chaetomium thermophilum)
- Utp21 (115 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s28-like protein (7 kDa, Protein from Chaetomium thermophilum)
- Utp14 (104 kDa, Protein from Chaetomium thermophilum)
- Nop1 (32 kDa, Protein from Chaetomium thermophilum)
- Nop56 (57 kDa, Protein from Chaetomium thermophilum)
- Imp4 (34 kDa, Protein from Chaetomium thermophilum)
- Snu13 (13 kDa, Protein from Chaetomium thermophilum)
- 90s pre-ribosome, state a (Complex from Chaetomium thermophilum)
- U3 small nucleolar rna-associated protein 11 (31 kDa, Protein from Chaetomium thermophilum)
- Rps18 (17 kDa, Protein from Chaetomium thermophilum)
- Utp6 (46 kDa, Protein from Chaetomium thermophilum)
- Utp18 (69 kDa, Protein from Chaetomium thermophilum)
- Utp7 (63 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s5-like protein (23 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s13-like protein (16 kDa, Protein from Chaetomium thermophilum)
- Periodic tryptophan protein 2-like protein (100 kDa, Protein from Chaetomium thermophilum)
- Fcf2 (22 kDa, Protein from Chaetomium thermophilum)
- Utp12 (107 kDa, Protein from Chaetomium thermophilum)
- Mpp10 (86 kDa, Protein from Chaetomium thermophilum)
- Utp15 (60 kDa, Protein from Chaetomium thermophilum)
- Sof1 (50 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s22-like protein (14 kDa, Protein from Chaetomium thermophilum)
- Emg1 (27 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s9-like protein (22 kDa, Protein from Chaetomium thermophilum)
- Utp5 (44 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s16-like protein (15 kDa, Protein from Chaetomium thermophilum)
- Utp30 (43 kDa, Protein from Chaetomium thermophilum)
Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state b
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6rxz
Q-score: 0.227
Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E
Mol Cell (2019) 75 pp. 1256-1269.e7 [ DOI: doi:10.1016/j.molcel.2019.06.032 Pubmed: 31378463 ]
- Utp24 (22 kDa, Protein from Chaetomium thermophilum)
- Utp13 (100 kDa, Protein from Chaetomium thermophilum)
- Rrp9 (69 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s23-like protein (15 kDa, Protein from Chaetomium thermophilum)
- Putative nucleolar protein (57 kDa, Protein from Chaetomium thermophilum)
- Utp17 (105 kDa, Protein from Chaetomium thermophilum)
- Kre33 (119 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s7 (23 kDa, Protein from Chaetomium thermophilum)
- U3 small nucleolar rna-associated protein 22 (131 kDa, Protein from Chaetomium thermophilum)
- Imp3 (21 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s14-like protein (16 kDa, Protein from Chaetomium thermophilum)
- Putative u3 small nucleolar ribonucleoprotein protein (86 kDa, Protein from Chaetomium thermophilum)
- U3 snorna (73 kDa, RNA from Chaetomium thermophilum)
- Bms1 (134 kDa, Protein from Chaetomium thermophilum)
- Faf1 (34 kDa, Protein from Chaetomium thermophilum)
- Utp4 (97 kDa, Protein from Chaetomium thermophilum)
- Enp1 (54 kDa, Protein from Chaetomium thermophilum)
- Utp2 (103 kDa, Protein from Chaetomium thermophilum)
- Utp3 (73 kDa, Protein from Chaetomium thermophilum)
- Zinc ion (65 Da, Ligand)
- Rrp5 (197 kDa, Protein from Chaetomium thermophilum)
- Pre-rrna-processing protein pno1 (28 kDa, Protein from Chaetomium thermophilum)
- Putative u3 small nucleolar ribonucleoprotein (34 kDa, Protein from Chaetomium thermophilum)
- Noc4 (63 kDa, Protein from Chaetomium thermophilum)
- Rna 3'-terminal phosphate cyclase-like protein (43 kDa, Protein from Chaetomium thermophilum)
- Nop58 (64 kDa, Protein from Chaetomium thermophilum)
- Krr1 small subunit processome component (37 kDa, Protein from Chaetomium thermophilum)
- Rrp7 (36 kDa, Protein from Chaetomium thermophilum)
- Utp8 (102 kDa, Protein from Chaetomium thermophilum)
- Bfr2 (69 kDa, Protein from Chaetomium thermophilum)
- Utp16 (40 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s28-like protein (7 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s11-like protein (18 kDa, Protein from Chaetomium thermophilum)
- Utp14 (104 kDa, Protein from Chaetomium thermophilum)
- Nop1 (32 kDa, Protein from Chaetomium thermophilum)
- Snu13 (13 kDa, Protein from Chaetomium thermophilum)
- U3 small nucleolar rna-associated protein 11 (31 kDa, Protein from Chaetomium thermophilum)
- Rps18 (17 kDa, Protein from Chaetomium thermophilum)
- Utp6 (46 kDa, Protein from Chaetomium thermophilum)
- Utp18 (69 kDa, Protein from Chaetomium thermophilum)
- Utp7 (63 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s5-like protein (23 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s13-like protein (16 kDa, Protein from Chaetomium thermophilum)
- Utp5 (44 kDa, Protein from Chaetomium thermophilum)
- Ribosome biogenesis protein enp2 (75 kDa, Protein from Chaetomium thermophilum)
- Periodic tryptophan protein 2-like protein (100 kDa, Protein from Chaetomium thermophilum)
- Utp10 (198 kDa, Protein from Chaetomium thermophilum)
- 90s pre-ribosome, state b (Complex from Chaetomium thermophilum)
- Fcf2 (22 kDa, Protein from Chaetomium thermophilum)
- Putative u3 snornp protein (115 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s4 (29 kDa, Protein from Chaetomium thermophilum)
- Utp12 (107 kDa, Protein from Chaetomium thermophilum)
- 35s ribosomal rna (749 kDa, RNA from Chaetomium thermophilum)
- 40s ribosomal protein s8 (23 kDa, Protein from Chaetomium thermophilum)
- Utp15 (60 kDa, Protein from Chaetomium thermophilum)
- Sof1 (50 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s22-like protein (14 kDa, Protein from Chaetomium thermophilum)
- U3 small nucleolar ribonucleoprotein protein lcp5-like protein (42 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s6 (27 kDa, Protein from Chaetomium thermophilum)
- Emg1 (27 kDa, Protein from Chaetomium thermophilum)
- Utp20 (295 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s9-like protein (22 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s24 (15 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s16-like protein (15 kDa, Protein from Chaetomium thermophilum)
- Utp30 (43 kDa, Protein from Chaetomium thermophilum)
Conformational switches control early maturation of the eukaryotic small ribosomal subunit
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6nd4
Q-score: 0.26
Hunziker M, Barandun J, Buzovetsky O, Steckler C, Molina H, Klinge S
eLife (2019) 8 [ DOI: doi:10.7554/eLife.45185 Pubmed: 31206356 ]
- Nop56 (55 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Mpp10 (67 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp17 (96 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Snu13 (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nop58 (53 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp1 (104 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5' ets particle (Complex from Saccharomyces cerevisiae BY4741)
- Utp15 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Bud21 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- U3 snorna (46 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Utp8 (49 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nop1.1 (34 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5'ets rrna (220 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Unidentified fragment (2 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp12 (102 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Sof1 (56 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 18s rrna 5' domain start (3 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Utp5 (72 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp24 (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp21 (104 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp13 (68 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp18 (66 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp10 (200 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp9 (19 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Imp3 (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp7 (62 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrp9 (65 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp6 (46 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp4 (81 kDa, Protein from Saccharomyces cerevisiae BY4741)
Cryo-EM structure of the 90S pre-ribosome from Chaetomium thermophilum
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 5oql
Q-score: 0.382
Cheng J, Kellner N, Berninghausen O, Hurt E, Beckmann R
Nat Struct Mol Biol (2017) 24 pp. 954-964 [ Pubmed: 28967883 DOI: doi:10.1038/nsmb.3476 ]
- The 90s pre-ribosomne (Complex from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Putative u3 small nucleolar ribonucleoprotein (34 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rna cytidine acetyltransferase (119 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 35s rrna (827 kDa, RNA from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s9-like protein (22 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp24 (22 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s13-like protein (16 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s7-like protein (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp2 (103 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp15 (60 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Zinc ion (65 Da, Ligand)
- Utp18 (69 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Periodic tryptophan protein 2-like protein (100 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s8 (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- U3 snorna (87 kDa, RNA from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s1 (29 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rrp9 (69 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Sof1 (50 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s6 (27 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp13 (100 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s16-like protein (15 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- U3 small nucleolar rna-associated protein 11 (31 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp6 (46 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Putative u3 small nucleolar ribonucleoprotein protein (86 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s28-like protein (7 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Fcf2 (22 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s11-like protein (18 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Imp3 (21 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp3 (73 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Snu13 (13 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp14 (104 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Krr1 small subunit processome component (37 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp7 (63 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp30 (43 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Pre-rrna-processing protein pno1 (28 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp17 (105 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s5-like protein (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Emg1 (27 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s4 (29 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s22-like protein (14 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp4 (97 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Putative u3 snornp protein (115 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Bms1 (134 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp10 (198 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Faf1 (34 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rna 3'-terminal phosphate cyclase-like protein (43 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s24 (15 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nop1 (32 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp12 (107 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nop58 (64 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s23-like protein (15 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Putative nucleolar protein (57 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s14-like protein (16 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
The complete structure of the small subunit processome
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 5wlc
Q-score: 0.373
Barandun J, Chaker-Margot M, Hunziker M, Molloy KR, Chait BT, Klinge S
Nat Struct Mol Biol (2017) 24 pp. 944-953 [ Pubmed: 28945246 DOI: doi:10.1038/nsmb.3472 ]
- Rrp7 (34 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp30 (31 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Bud21 (23 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Mpp10 (67 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Enp1 (55 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Snu13 (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 18s pre-rrna (582 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Utp11 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps9_us4 (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp13 (75 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp14 (101 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps22_us8 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Kre33 (95 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrt14 (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Krr1 (37 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Faf1 (38 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- Rps5_us7 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps24_es24 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp1 (104 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Sas10 (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Unassigned peptides (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps18_us13 (16 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nop56 (56 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps11_us17 (17 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp15 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rcl1 (40 kDa, Protein from Saccharomyces cerevisiae BY4741)
- U3 snorna (106 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Rps8_es8 (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp17 (100 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps16_us9 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Imp4 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps4_es4 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nop1 (34 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp9 (64 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp6 (48 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Lcp5 (40 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp4 (87 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Sof1 (56 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Pno1 (30 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp20 (83 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps7_es7 (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp10 (200 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp5 (72 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp24 (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp12 (105 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Bms1 (135 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp21 (104 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp8 (65 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nop14 (85 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps14_us11 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp18 (66 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps6_es6 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps23_us12 (16 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Imp3 (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps28_es28 (7 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp22 (140 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Enp2 (81 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nop58 (53 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Emg1 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Noc4 (58 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Utp7 (62 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Fcf2 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrp9 (65 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rps13_us15 (17 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrp5 (193 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Small subunit processome (Complex from Saccharomyces cerevisiae BY4741)
- 5' ets (225 kDa, RNA from Saccharomyces cerevisiae BY4741)
Cryo-EM structure of the 90S small subunit pre-ribosome (Dhr1-depleted, Enp1-TAP, state 1)
Resolution: 8.7 Å
EM Method: Single-particle
Fitted PDBs: 5wyj
Q-score: 0.16
Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K
eLife (2017) 6 [ Pubmed: 28244370 DOI: doi:10.7554/eLife.22086 ]
- 40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s13 (17 kDa, Protein from Baker's yeast)
- 13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Baker's yeast)
- Utp5 (54 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
- 18s ribosomal rna (583 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s9-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from Baker's yeast)
- Helical domain protein (137 kDa, Protein from Baker's yeast)
- Utp11 (21 kDa, Protein from Baker's yeast)
- Utp17 (76 kDa, Protein from Baker's yeast)
- Enp2 (60 kDa, Protein from Baker's yeast)
- Utp15 (43 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 13 (91 kDa, Protein from Baker's yeast)
- U3 small nucleolar ribonucleoprotein protein imp3 (21 kDa, Protein from Baker's yeast)
- Periodic tryptophan protein 2 (104 kDa, Protein from Baker's yeast)
- U3 small nucleolar ribonucleoprotein protein imp4 (33 kDa, Protein from Baker's yeast)
- Sof1 (41 kDa, Protein from Baker's yeast)
- Rrna-processing protein fcf1 (21 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 21 (104 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 22 (140 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s23-a (16 kDa, Protein from Baker's yeast)
- Helical domain protein (84 kDa, Protein from Baker's yeast)
- Ribosomal rna small subunit methyltransferase nep1 (27 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein utp30 (31 kDa, Protein from Baker's yeast)
- Pre-rrna-processing protein pno1 (30 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 18 (66 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s6-a (27 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
- Mpp10,u3 small nucleolar rna-associated protein mpp10 (54 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s28-a (7 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein bms1 (135 kDa, Protein from Baker's yeast)
- Ribosomal rna-processing protein 7 (34 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
- Rna 3'-terminal phosphate cyclase-like protein (40 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 12 (106 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
- Unassigned helices (87 kDa, Protein from Baker's yeast)
- 90s small subunit pre-ribosome (dhr1-depleted, enp1-tap) (Complex from Saccharomyces cerevisiae)
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Baker's yeast)
- Utp8 (60 kDa, Protein from Baker's yeast)
- Essential nuclear protein 1 (55 kDa, Protein from Baker's yeast)
- Krr1 small subunit processome component (37 kDa, Protein from Baker's yeast)
- Utp20 (212 kDa, Protein from Baker's yeast)
- Rrna biogenesis protein rrp5 (193 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s30-a (7 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s5 (25 kDa, Protein from Baker's yeast)
- Utp7 (47 kDa, Protein from Baker's yeast)
- 5ets rna (225 kDa, RNA from Baker's yeast)
- Utp9 (48 kDa, Protein from Baker's yeast)
- Rrna 2'-o-methyltransferase fibrillarin (34 kDa, Protein from Baker's yeast)
- Ribosomal rna-processing protein 9 (65 kDa, Protein from Baker's yeast)
- Nucleolar protein 58 (57 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
- U3 rna (106 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s14-a (14 kDa, Protein from Baker's yeast)
- Nucleolar protein 56 (56 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s12 (15 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 10,utp10 (173 kDa, Protein from Baker's yeast)
- Utp4 (66 kDa, Protein from Baker's yeast)