Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8v4y
Q-score: 0.53
Chio US, Palovcak E, Smith AAA, Autzen H, Munoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
Nat Commun (2024) 15 pp. 2225-2225 [ Pubmed: 38472177 DOI: doi:10.1038/s41467-024-46178-y ]
- Beryllium trifluoride ion (66 Da, Ligand)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (reverse strand) (64 kDa, DNA from synthetic construct)
- Nucleosome with xenopus laevis histones (200 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (forward strand) (63 kDa, DNA from synthetic construct)
- Histone h2b (13 kDa, Protein from Xenopus laevis)
- Snf2h (100 kDa, Complex from Homo sapiens)
- Atp-dependent chromatin remodeler snf2h in complex with nucleosome (300 kDa, Complex)
- Histone h2a type 1 (13 kDa, Protein from Xenopus laevis)
- Swi/snf-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 (122 kDa, Protein from Homo sapiens)
Cryo-EM structure of SNF2h-nucleosome complex (consensus structure)
Chio US, Palovcak E, Smith AAA, Autzen H, Munoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
Nat Commun (2024) 15 pp. 2225-2225 [ Pubmed: 38472177 DOI: doi:10.1038/s41467-024-46178-y ]
- Histone h3.2 (Protein from Xenopus laevis)
- Widom 601 dna (147-mer) plus 60 base pairs flanking dna forward (DNA from synthetic construct)
- Histone h4 (Protein from Xenopus laevis)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Widom 601 dna (147-mer) plus 60 base pairs flanking dna reverse (DNA from synthetic construct)
- Swi/snf-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 (Protein from Homo sapiens)
- Snf2h (Complex from Homo sapiens)
- Atp-dependent chromatin remodeler snf2h in complex with nucleosome (100 kDa, Complex from Homo sapiens)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Nucleosome with xenopus laevis histones (Complex from Xenopus laevis)
Cryo-EM structure of SNF2h-nucleosome complex (single-bound structure)
Chio US, Palovcak E, Smith AAA, Autzen H, Munoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
Nat Commun (2024) 15 pp. 2225-2225 [ Pubmed: 38472177 DOI: doi:10.1038/s41467-024-46178-y ]
- Histone h3.2 (Protein from Xenopus laevis)
- Nucleosome with xenopus laevis histones (200 kDa, Complex)
- Snf2h (100 kDa, Complex from Homo sapiens)
- Histone h4 (Protein from Xenopus laevis)
- Atp-dependent chromatin remodeler snf2h in complex with nucleosome (300 kDa, Complex)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Widom 601 dna (147-mer) plus 60 base pairs flanking dna forward strand (DNA from synthetic construct)
- Swi/snf-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 (Protein from Homo sapiens)
- Widom 601 dna (147-mer) plus 60 base pairs flanking dna reverse strand (DNA from synthetic construct)
- Histone h2b 1.1 (Protein from Xenopus laevis)
Cryo-EM structure of doubly-bound SNF2h-nucleosome complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8v6v
Q-score: 0.477
Chio US, Palovcak E, Smith AAA, Autzen H, Munoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
Nat Commun (2024) 15 pp. 2225-2225 [ Pubmed: 38472177 DOI: doi:10.1038/s41467-024-46178-y ]
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (reverse strand) (64 kDa, DNA from synthetic construct)
- Nucleosome with xenopus laevis histones (200 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (forward strand) (63 kDa, DNA from synthetic construct)
- Histone h2b (13 kDa, Protein from Xenopus laevis)
- Snf2h (100 kDa, Complex from Homo sapiens)
- Atp-dependent chromatin remodeler snf2h in complex with nucleosome (300 kDa, Complex)
- Histone h2a type 1 (13 kDa, Protein from Xenopus laevis)
- Swi/snf-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 (122 kDa, Protein from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8v7l
Q-score: 0.456
Chio US, Palovcak E, Smith AAA, Autzen H, Munoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
Nat Commun (2024) 15 pp. 2225-2225 [ Pubmed: 38472177 DOI: doi:10.1038/s41467-024-46178-y ]
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Magnesium ion (24 Da, Ligand)
- Nucleosome with xenopus laevis histones (200 kDa, Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (forward strand) (63 kDa, DNA from synthetic construct)
- Histone h2b (13 kDa, Protein from Xenopus laevis)
- Snf2h (100 kDa, Complex from Homo sapiens)
- Atp-dependent chromatin remodeler snf2h in complex with nucleosome (300 kDa, Complex)
- Histone h2a type 1 (13 kDa, Protein from Xenopus laevis)
- Swi/snf-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 (122 kDa, Protein from Homo sapiens)
- Widom 601 dna (147-mer) plus 60 base pairs flanking dna (reverse strand) (64 kDa, DNA from synthetic construct)
Cryo-EM structure of doubly-bound SNF2h-nucleosome complex (conformation 1)
Chio US, Palovcak E, Smith AAA, Autzen H, Munoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
Nat Commun (2024) 15 pp. 2225-2225 [ Pubmed: 38472177 DOI: doi:10.1038/s41467-024-46178-y ]
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (reverse strand) (DNA from synthetic construct)
- Histone h3.2 (Protein from Xenopus laevis)
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (forward strand) (DNA from synthetic construct)
- Nucleosome with xenopus laevis histones (200 kDa, Complex)
- Snf2h (100 kDa, Complex from Homo sapiens)
- Histone h4 (Protein from Xenopus laevis)
- Atp-dependent chromatin remodeler snf2h in complex with nucleosome (300 kDa, Complex)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Swi/snf-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 (Protein from Homo sapiens)
- Histone h2b 1.1 (Protein from Xenopus laevis)
Cryo-EM structure of doubly-bound SNF2h-nucleosome complex (conformation 2)
Chio US, Palovcak E, Smith AAA, Autzen H, Munoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
Nat Commun (2024) 15 pp. 2225-2225 [ Pubmed: 38472177 DOI: doi:10.1038/s41467-024-46178-y ]
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (reverse strand) (DNA from synthetic construct)
- Histone h3.2 (Protein from Xenopus laevis)
- Widom 601 dna (147-mer) with 60 base pairs flanking dna (forward strand) (DNA from synthetic construct)
- Nucleosome with xenopus laevis histones (200 kDa, Complex)
- Snf2h (100 kDa, Complex from Homo sapiens)
- Histone h4 (Protein from Xenopus laevis)
- Atp-dependent chromatin remodeler snf2h in complex with nucleosome (300 kDa, Complex)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Swi/snf-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 (Protein from Homo sapiens)
- Histone h2b 1.1 (Protein from Xenopus laevis)
H3K36me3 nucleosome-LEDGF/p75 PWWP domain complex
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8pc5
Q-score: 0.521
Koutna E, Lux V, Kouba T, Skerlova J, Novacek J, Srb P, Hexnerova R, Svachova H, Kukacka Z, Novak P, Fabry M, Poepsel S, Veverka V
Nucleic Acids Res (2023) 51 pp. 10011-10025 [ DOI: doi:10.1093/nar/gkad674 Pubmed: 37615563 ]
- Dna (Complex from synthetic construct)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Widom 601 dna (147-mer) (45 kDa, DNA from synthetic construct)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Pc4 and sfrs1-interacting protein (60 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Widom 601 dna (147-mer) (45 kDa, DNA from synthetic construct)
- Pc4 and sfrs1-interacting protein (Complex from Homo sapiens)
- H3k36me3 nucleosome-ledgf/p75 pwwp domain complex (Complex)
H3K36me3 nucleosome-LEDGF/p75 PWWP domain complex - pose 2
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 8pc6
Q-score: 0.484
Koutna E, Lux V, Kouba T, Skerlova J, Novacek J, Srb P, Hexnerova R, Svachova H, Kukacka Z, Novak P, Fabry M, Poepsel S, Veverka V
Nucleic Acids Res (2023) 51 pp. 10011-10025 [ DOI: doi:10.1093/nar/gkad674 Pubmed: 37615563 ]
- Dna (Complex from synthetic construct)
- H3k36me3 nucleosome-ledgf/p75 pwwp domain complex - pose 2 (Complex)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Pc4 and sfrs1-interacting protein (60 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Widom 601 dna (147-mer) (45 kDa, DNA from synthetic construct)
- Pc4 and sfrs1-interacting protein (Complex from Homo sapiens)
- Widom 601 dna (147-mer) (45 kDa, DNA from synthetic construct)
H3K36me2 nucleosome-LEDGF/p75 PWWP domain complex
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 8peo
Q-score: 0.548
Koutna E, Lux V, Kouba T, Skerlova J, Novacek J, Srb P, Hexnerova R, Svachova H, Kukacka Z, Novak P, Fabry M, Poepsel S, Veverka V
Nucleic Acids Res (2023) 51 pp. 10011-10025 [ DOI: doi:10.1093/nar/gkad674 Pubmed: 37615563 ]
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- H3k36me2 nucleosome-ledgf/p75 pwwp domain complex (Complex from Xenopus laevis)
- Pc4 and sfrs1-interacting protein (60 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Widom 601 dna (147-mer) (45 kDa, DNA from synthetic construct)
- Widom 601 dna (147-mer) (45 kDa, DNA from synthetic construct)