Structure of the mu opioid receptor bound to the antagonist nanobody NbE
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8qot
Q-score: 0.413
Yu J, Kumar A, Zhang X, Martin C, Van Holsbeeck K, Raia P, Koehl A, Laeremans T, Steyaert J, Manglik A, Ballet S, Boland A, Stoeber M
Nat Commun (2024) 15 pp. 8687-8687 [ Pubmed: 39384768 DOI: doi:10.1038/s41467-024-52947-6 ]
- Nabfab lc (25 kDa, Protein from synthetic construct)
- Anti-fab nanobody (17 kDa, Protein from Lama glama)
- Nabfab hc (28 kDa, Protein from synthetic construct)
- Mu-type opioid receptor (53 kDa, Protein from Mus musculus)
- Mor-nbe-nabfab-antifab complex (120 kDa, Complex from Mus musculus)
- Nanobody e (nbe) (18 kDa, Protein from Lama glama)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7php
Q-score: 0.505
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34782475 DOI: doi:10.1073/pnas.2115435118 ]
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Multidrug resistance protein norm (52 kDa, Protein from Vibrio cholerae RC385)
- Norm-nb17_4 (14 kDa, Protein from synthetic construct)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
- Complex of vibrio cholerae mate transporter norm, chimeric nanobody norm-nb17_4, nabfab, and anti-fab nanobody (Complex from Vibrio cholerae RC385)
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34782475 DOI: doi:10.1073/pnas.2115435118 ]
- Multidrug resistance protein norm (Complex from synthetic construct)
- Nabfab lc (Complex from synthetic construct)
- Norm-nb17_4 (Complex from Lama glama)
- Anti-fab nanobody (Complex)
- Nabfab hc (Complex from synthetic construct)
- Complex of vibrio cholerae mate transporter norm, chimeric nanobody norm-nb17_4, nabfab, and anti-fab nanobody (Complex from Vibrio cholerae RC385)
Resolution: 8.45 Å
EM Method: Single-particle
Fitted PDBs: 7phq
Q-score: 0.111
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34782475 DOI: doi:10.1073/pnas.2115435118 ]
- Complex of staphylococcus capitis divalent metal ion transporter (dmt), chimeric nanobody dmtnb16_4, nabfab, and anti-fab nanobody (Complex from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Dmt-nb16_4 (14 kDa, Protein from synthetic construct)
- Divalent metal cation transporter mnth (47 kDa, Protein from Staphylococcus capitis)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 7pij
Q-score: 0.422
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34782475 DOI: doi:10.1073/pnas.2115435118 ]
- Water (18 Da, Ligand)
- Complex of staphylococcus capitis divalent metal ion transporter (dmt), chimeric nanobody dmtnb16_4, nabfab, and anti-fab nanobody (Complex from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Divalent metal cation transporter mnth (47 kDa, Protein from Staphylococcus capitis)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Dmtnb16_4 (14 kDa, Protein from synthetic construct)