Cryo-EM structure of ETAR bound with Macitentan
Hou J, Liu S, Zhang X, Tu G, Wu L, Zhang Y, Yang H, Li X, Liu J, Jiang L, Tan Q, Bai F, Liu Z, Miao C, Hua T, Luo Z
Cell Discov (2024) 10 pp. 79-79 [ DOI: doi:10.1038/s41421-024-00705-9 Pubmed: 39075075 ]
- Macitentan (588 Da, Ligand)
- Endoglucanase h,endothelin-1 receptor,soluble cytochrome b562 (89 kDa, Protein from Homo sapiens)
- Anti-bril fab light chain (25 kDa, Protein from Homo sapiens)
- Anti-fab nanobody (15 kDa, Protein from synthetic construct)
- Anti-bril fab heavy chain (28 kDa, Protein from Homo sapiens)
- Complex of endothelin receptor type a with macitentan (Complex from Homo sapiens)
Cryo-EM structure of ETAR bound with Ambrisentan
Hou J, Liu S, Zhang X, Tu G, Wu L, Zhang Y, Yang H, Li X, Liu J, Jiang L, Tan Q, Bai F, Liu Z, Miao C, Hua T, Luo Z
Cell Discov (2024) 10 pp. 79-79 [ DOI: doi:10.1038/s41421-024-00705-9 Pubmed: 39075075 ]
- Complex of endothelin receptor type a with ambrisentan (Complex from Homo sapiens)
- Endoglucanase h,endothelin-1 receptor,soluble cytochrome b562 (89 kDa, Protein from Homo sapiens)
- Anti-bril fab light chain (25 kDa, Protein from Homo sapiens)
- Anti-bril fab heavy chain (28 kDa, Protein from Homo sapiens)
- Anti-fab nanobody (15 kDa, Protein from synthetic construct)
- Ambrisentan (378 Da, Ligand)
Cryo-EM structure of ETAR bound with Zibotentan
Hou J, Liu S, Zhang X, Tu G, Wu L, Zhang Y, Yang H, Li X, Liu J, Jiang L, Tan Q, Bai F, Liu Z, Miao C, Hua T, Luo Z
Cell Discov (2024) 10 pp. 79-79 [ DOI: doi:10.1038/s41421-024-00705-9 Pubmed: 39075075 ]
- Zibotentan (424 Da, Ligand)
- Complex of endothelin receptor type a with zibotentan (Complex from Homo sapiens)
- Endoglucanase h,endothelin-1 receptor,soluble cytochrome b562 (89 kDa, Protein from Homo sapiens)
- Anti-bril fab light chain (25 kDa, Protein from Homo sapiens)
- Anti-bril fab heavy chain (28 kDa, Protein from Homo sapiens)
- Anti-fab nanobody (15 kDa, Protein from synthetic construct)
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 9cmi
Q-score: 0.468
Rathnayake SS, Erramilli SK, Kossiakoff AA, Vecchio AJ
Structure (2024) 32 pp. 1936- [ Pubmed: 39383874 DOI: doi:10.1016/j.str.2024.09.015 ]
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Water (18 Da, Ligand)
- Heat-labile enterotoxin b chain (33 kDa, Protein from Clostridium perfringens)
- Cop-1 sfab heavy chain (28 kDa, Protein from Homo sapiens)
- Human claudin-4 complex with clostridium perfringens enterotoxin, sfab cop-1, and nanobody (120 kDa, Complex from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from Homo sapiens)
- Claudin-4 (22 kDa, Protein from Homo sapiens)
- Cop-1 sfab light chain (25 kDa, Protein from Homo sapiens)
Structure of AT118-H Nanobody Antagonist in Complex with the Angiotensin II Type I Receptor
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8th3
Q-score: 0.532
Skiba MA, Sterling SM, Rawson S, Zhang S, Xu H, Jiang H, Nemeth GR, Gilman MSA, Hurley JD, Shen P, Staus DP, Kim J, McMahon C, Lehtinen MK, Rockman HA, Barth P, Wingler LM, Kruse AC
Nat Chem Biol (2024) 20 pp. 1577-1585 [ DOI: doi:10.1038/s41589-024-01620-6 Pubmed: 38744986 ]
- Bag2 anti-bril fab heavy chain (24 kDa, Protein from synthetic construct)
- Bag2 anti-bril fab light chain (23 kDa, Protein from synthetic construct)
- Cholesterol hemisuccinate (486 Da, Ligand)
- At118-h nanobody, type-1 angiotensin ii receptor, soluble cytochrome b562 complex (65 kDa, Protein from Homo sapiens)
- At118-h nanobody antagonist in complex with the angiotensin ii type i receptor bril fusion, anti-bril fab, and anti-fab nanobody (Complex from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8th4
Q-score: 0.495
Skiba MA, Sterling SM, Rawson S, Zhang S, Xu H, Jiang H, Nemeth GR, Gilman MSA, Hurley JD, Shen P, Staus DP, Kim J, McMahon C, Lehtinen MK, Rockman HA, Barth P, Wingler LM, Kruse AC
Nat Chem Biol (2024) 20 pp. 1577-1585 [ DOI: doi:10.1038/s41589-024-01620-6 Pubmed: 38744986 ]
- Bag2 anti-bril fab heavy chain (24 kDa, Protein from synthetic construct)
- Bag2 anti-bril fab light chain (23 kDa, Protein from synthetic construct)
- Type-1 angiotensin ii receptor soluble cytochrome b562 complex (49 kDa, Protein from Homo sapiens)
- At118-l nanobody (15 kDa, Protein from synthetic construct)
- At118-l nanobody antagonist in complex with the angiotensin ii type i receptor bril fusion protein, anti-bril fab, anti-fab nanobody, and losartan (Complex from Homo sapiens)
- [2-butyl-5-chloranyl-3-[[4-[2-(2h-1,2,3,4-tetrazol-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol (422 Da, Ligand)
Resolution: 4.16 Å
EM Method: Single-particle
Fitted PDBs: 9bei
Q-score: 0.276
Goverde CA, Pacesa M, Goldbach N, Dornfeld LJ, Balbi PEM, Georgeon S, Rosset S, Kapoor S, Choudhury J, Dauparas J, Schellhaas C, Kozlov S, Baker D, Ovchinnikov S, Vecchio AJ, Correia BE
Nature (2024) 631 pp. 449-458 [ DOI: doi:10.1038/s41586-024-07601-y Pubmed: 38898281 ]
- Cop-2 fab light chain (23 kDa, Protein from Escherichia coli)
- Claudin-4 (22 kDa, Protein from Homo sapiens)
- Synthetic human claudin-4 complex with clostridium perfringens enterotoxin c-terminal domain, sfab cop-2, and nanobody against cop-2 (103 kDa, Complex from Homo sapiens)
- Cop-2 fab heavy chain (25 kDa, Protein from Escherichia coli)
- Heat-labile enterotoxin b chain (14 kDa, Protein from Clostridium perfringens)
- Anti-fab nanobody (13 kDa, Protein from Escherichia coli)
Structure of the mu opioid receptor bound to the antagonist nanobody NbE
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8qot
Q-score: 0.413
Yu J, Kumar A, Zhang X, Martin C, Van Holsbeeck K, Raia P, Koehl A, Laeremans T, Steyaert J, Manglik A, Ballet S, Boland A, Stoeber M
Nat Commun (2024) 15 pp. 8687-8687 [ Pubmed: 39384768 DOI: doi:10.1038/s41467-024-52947-6 ]
- Nabfab hc (28 kDa, Protein from synthetic construct)
- Anti-fab nanobody (17 kDa, Protein from Lama glama)
- Nanobody e (nbe) (18 kDa, Protein from Lama glama)
- Mor-nbe-nabfab-antifab complex (120 kDa, Complex from Mus musculus)
- Mu-type opioid receptor (53 kDa, Protein from Mus musculus)
- Nabfab lc (25 kDa, Protein from synthetic construct)
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 8u4v
Q-score: 0.572
Erramilli SK, Dominik PK, Ogbu CP, Kossiakoff AA, Vecchio AJ
Commun Biol (2024) 7 pp. 733-733 [ DOI: doi:10.1038/s42003-024-06437-6 Pubmed: 38886509 ]
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Claudin-4 (22 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Heat-labile enterotoxin b chain (14 kDa, Protein from Clostridium perfringens)
- Cop-1 sfab heavy chain (28 kDa, Protein from Homo sapiens)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- Cop-1 sfab light chain (23 kDa, Protein from Homo sapiens)
- Human claudin-4 complex with clostridium perfringens enterotoxin c-terminal domain, sfab cop-1, and nanobody (100 kDa, Complex from Homo sapiens)
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 7yud
Q-score: 0.53
Duan Y, Leong NCP, Zhao J, Zhang Y, Nguyen DT, Ha HTT, Wang N, Xia R, Xu Z, Ma Z, Qian Y, Yin H, Zhu X, Zhang A, Guo C, Xia Y, Nguyen LN, He Y
Cell Res (2024) 34 pp. 177-180 [ DOI: doi:10.1038/s41422-023-00913-0 Pubmed: 38123825 ]
- Nbfab-h-chain (26 kDa, Protein from synthetic construct)
- Tc-nb8 (14 kDa, Protein from Lama glama)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Spns2 nanobody complex (Complex from Lama glama)
- (2~{s})-2-azanyl-4-(4-octylphenyl)-2-[[oxidanyl-bis(oxidanylidene)-$l^{6}-phosphanyl]oxymethyl]butan-1-ol (387 Da, Ligand)
- Sphingosine-1-phosphate transporter spns2 (58 kDa, Protein from Homo sapiens)
- Nbfab l-chain (23 kDa, Protein from synthetic construct)