Cryo-EM structure of GSK682753A-bound EBI2/GPR183
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 7tuy
Q-score: 0.471
Structure (2022) 30 pp. 1016- [ Pubmed: 35537452 DOI: doi:10.1016/j.str.2022.04.006 ]
- 8-[(2e)-3-(4-chlorophenyl)prop-2-enoyl]-3-[(3,4-dichlorophenyl)methyl]-1-oxa-3,8-diazaspiro[4.5]decan-2-one (479 Da, Ligand)
- G-protein coupled receptor 183,soluble cytochrome b562 fusion (54 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 183,soluble cytochrome b562 fusion (Complex from Homo sapiens)
- Anti-fab nanobody (15 kDa, Protein from synthetic construct)
- Anti-bril fab heavy chain (24 kDa, Protein from Homo sapiens)
- Anti-fab nanobody (Complex from synthetic construct)
- Complex of gsk682753a-bound ebi2/gpr183-bril chimera with anti-bril fab and anti-fab nanobody (Complex)
- Anti-bril fab heavy chain, anti-bril fab light chain (Complex from Homo sapiens)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
SARS-CoV-2 Omicron Variant S Trimer complexed with two JMB2002 Fab
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 7wpe
Q-score: 0.338
Yin W, Xu Y, Xu P, Cao X, Wu C, Gu C, He X, Wang X, Huang S, Yuan Q, Wu K, Hu W, Huang Z, Liu J, Wang Z, Jia F, Xia K, Liu P, Wang X, Song B, Zheng J, Jiang H, Cheng X, Jiang Y, Deng SJ, Xu HE
Science (2022) 375 pp. 1048-1053 [ Pubmed: 35133176 DOI: doi:10.1126/science.abn8863 ]
- Spike glycoprotein (133 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Anti-fab nanobody (Complex)
- Sars-cov-2 omicron variant s (Complex from Mus musculus)
- Jmb2002 fab heavy chain (25 kDa, Protein from Mus musculus)
- Jmb2002 fab (Complex from Lama glama)
- Sars-cov-2 omicron variant s trimer complexed with jmb2002 fab (Complex from Severe acute respiratory syndrome coronavirus)
- Anti-fab nanobody (14 kDa, Protein from Lama glama)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Jmb2002 fab light chian (23 kDa, Protein from Mus musculus)
SARS-CoV-2 Omicron Variant S Trimer complexed with one JMB2002 Fab
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 7wpd
Q-score: 0.265
Yin W, Xu Y, Xu P, Cao X, Wu C, Gu C, He X, Wang X, Huang S, Yuan Q, Wu K, Hu W, Huang Z, Liu J, Wang Z, Jia F, Xia K, Liu P, Wang X, Song B, Zheng J, Jiang H, Cheng X, Jiang Y, Deng SJ, Xu HE
Science (2022) 375 pp. 1048-1053 [ Pubmed: 35133176 DOI: doi:10.1126/science.abn8863 ]
- Anti fab nanobody (Complex)
- Spike glycoprotein (133 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Jmb2002 fab heavy chain (25 kDa, Protein from Mus musculus)
- Sars-cov-2 omicron variant s trimer complexed with jmb2002 fab (Complex from Severe acute respiratory syndrome coronavirus 2)
- Jmb2002 fab (Complex from Lama glama)
- Sars-cov-2 omicron variant s trimer (Complex from Mus musculus)
- Anti-fab nanobody (14 kDa, Protein from Lama glama)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Jmb2002 fab light chian (23 kDa, Protein from Mus musculus)
SARS-CoV-2 Omicron Variant S Trimer complexed with three JMB2002 Fab
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 7wpf
Q-score: 0.226
Yin W, Xu Y, Xu P, Cao X, Wu C, Gu C, He X, Wang X, Huang S, Yuan Q, Wu K, Hu W, Huang Z, Liu J, Wang Z, Jia F, Xia K, Liu P, Wang X, Song B, Zheng J, Jiang H, Cheng X, Jiang Y, Deng SJ, Xu HE
Science (2022) 375 pp. 1048-1053 [ Pubmed: 35133176 DOI: doi:10.1126/science.abn8863 ]
- Anti fab nanobody (Complex)
- Spike glycoprotein (133 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 omicron variant s (Complex from Mus musculus)
- Jmb2002 fab heavy chain (25 kDa, Protein from Mus musculus)
- Sars-cov-2 omicron variant s trimer complexed with jmb2002 fab (Complex from Severe acute respiratory syndrome coronavirus 2)
- Jmb2002 fab (Complex from Lama glama)
- Jmb2002 fab light chain (23 kDa, Protein from Mus musculus)
- Anti-fab nanobody (14 kDa, Protein from Lama glama)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of human Frizzled5 by fiducial-assisted cryo-EM
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6ww2
Q-score: 0.42
Tsutsumi N, Mukherjee S, Waghray D, Janda CY, Jude KM, Miao Y, Burg JS, Aduri NG, Kossiakoff AA, Gati C, Garcia KC
eLife (2020) 9 [ Pubmed: 32762848 DOI: doi:10.7554/eLife.58464 ]
- Frizzled-5,soluble cytochrome b562 (74 kDa, Protein from Homo sapiens)
- Anti-fab nanobody (Complex)
- Frizzled-5 (Complex)
- Anti-bril fab (Complex)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- Anti-bril fab heavy chain (24 kDa, Protein from Homo sapiens)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Fzd5-bril/fab/nb complex (100 kDa, Complex from Homo sapiens)