Cryo-EM structure of SNAP-94847-bound MCHR1, S1 state
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 8yns
Q-score: 0.476
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Antibody 1b3 fab light chain (23 kDa, Protein from synthetic construct)
- Snap-94847-mchr1-fab1b3-glue complex (Complex from Homo sapiens)
- Melanin-concentrating hormone receptor 1,soluble cytochrome b562 (64 kDa, Protein from Homo sapiens)
- Glue molecule 4-9 (30 kDa, Protein from synthetic construct)
- Antibody 1b3 fab heavy chain (25 kDa, Protein from synthetic construct)
- ~{n}-[3-[1-[[4-[3,4-bis(fluoranyl)phenoxy]phenyl]methyl]piperidin-4-yl]-4-methyl-phenyl]-2-methyl-propanamide (478 Da, Ligand)
Cryo-EM structure of SNAP-94847-bound MCHR1, S2 state
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8ynt
Q-score: 0.422
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Antibody 1b3 fab light chain (23 kDa, Protein from synthetic construct)
- Snap-94847-mchr1-fab1b3-glue complex (Complex from Homo sapiens)
- Melanin-concentrating hormone receptor 1,soluble cytochrome b562 (64 kDa, Protein from Homo sapiens)
- Glue molecule 4-9 (30 kDa, Protein from synthetic construct)
- Antibody 1b3 fab heavy chain (25 kDa, Protein from synthetic construct)
- ~{n}-[3-[1-[[4-[3,4-bis(fluoranyl)phenoxy]phenyl]methyl]piperidin-4-yl]-4-methyl-phenyl]-2-methyl-propanamide (478 Da, Ligand)
Hemagglutinin H1 New Caledonia 1999 in complex with monoclonal antibody Fab 43_S0008
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 9b2m
Q-score: 0.377
Ataca S, Sangesland M, de Paiva Froes Rocha R, Torrents de la Pena A, Ronsard L, Boyoglu-Barnum S, Gillespie RA, Tsybovsky Y, Stephens T, Moin SM, Lederhofer J, Creanga A, Andrews SF, Barnes RM, Rohrer D, Lonberg N, Graham BS, Ward AB, Lingwood D, Kanekiyo M
Cell Rep (2024) 43 pp. 114990-114990 [ DOI: doi:10.1016/j.celrep.2024.114990 Pubmed: 39580804 ]
- Heavy chain monoclonal antibody fab 3_s0008 (24 kDa, Protein from Mus sp.)
- Light chain monoclonal antibody fab 3_s0008 (24 kDa, Protein from Mus sp.)
- Hemagglutinin h1 new caledonia 1999 in complex with monoclonal antibody fab 43_s0008 (330 kDa, Complex from Homo sapiens)
- Hemagglutinin ha1 chain (37 kDa, Protein from Influenza A virus)
- Hemagglutinin ha2 chain (25 kDa, Protein from Influenza A virus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
MCH-MCHR1-Gi complex, T1 state
Resolution: 2.61 Å
EM Method: Single-particle
Fitted PDBs: 8wwk
Q-score: 0.611
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mch-mchr1-gi complex t1 state (Complex from Homo sapiens)
- Melanin-concentrating hormone (2 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Fusion protein 1,melanin-concentrating hormone receptor 1,fusion protein 2 (68 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
MCH-MCHR1-Gi complex, T2 state
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 8wwl
Q-score: 0.573
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Melanin-concentrating hormone (2 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Fusion protein 1,melanin-concentrating hormone receptor 1,fusion protein 2 (68 kDa, Protein from Homo sapiens)
- Mch-mchr1-gi complex t2 state (Complex from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
MCH-MCHR1-Gi complex, L2 state
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8wwm
Q-score: 0.531
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mch-mchr1-gi complex l2 state (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Melanin-concentrating hormone (2 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Fusion protein 1,melanin-concentrating hormone receptor 1,fusion protein 2 (68 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 8wwn
Q-score: 0.6
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mch-mchr1-gi complex l1 state (Complex from Homo sapiens)
- Melanin-concentrating hormone (2 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Fusion protein 1,melanin-concentrating hormone receptor 1,fusion protein 2 (68 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8vcv
Q-score: 0.579
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Rabbit polyclonal fv heavy chain (10 kDa, Protein from Oryctolagus cuniculus)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (gpc-c epitope) (Complex from Oryctolagus cuniculus)
- Glycoprotein g2 (44 kDa, Protein from Lassa virus Josiah)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
- Rabbit polyclonal fv light chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8ve8
Q-score: 0.599
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Rabbit polyclonal fv heavy chain (10 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein g2 (44 kDa, Protein from Lassa virus Josiah)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (gp1-a epitope) (Complex from Oryctolagus cuniculus)
- Rabbit polyclonal fv light chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)