Structure of the FDX2-bound core ISC complex (proximal conformation)
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 8rmc
Q-score: 0.702
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the FDX2-bound core ISC complex (distal conformation)
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8rmd
Q-score: 0.642
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the core ISC complex under turnover conditions (frataxin-bound)
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8rme
Q-score: 0.615
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in proximal conformation)
Resolution: 2.33 Å
EM Method: Single-particle
Fitted PDBs: 8rmf
Q-score: 0.633
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in distal conformation)
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8rmg
Q-score: 0.598
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Infectious bronchitis virus core polymerase complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9cpo
Q-score: 0.477
Hoferle PJ, Anderson TK, Kirchdoerfer RN
bioRxiv (2024) [ DOI: doi:10.1101/2024.07.23.604833 Pubmed: 39091740 ]
- Non-structural protein 7 (8 kDa, Protein from Infectious bronchitis virus)
- Core polymerase complex of the infectious bronchitis virus composed of nsp12, nsp8, nsp7, and an dsrna substrate (194 kDa, Complex from Infectious bronchitis virus)
- Rna primer (9 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 8 (21 kDa, Protein from Infectious bronchitis virus)
- Zinc ion (65 Da, Ligand)
- Rna-directed rna polymerase nsp12 (107 kDa, Protein from Infectious bronchitis virus)
- Rna template (10 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
Resolution: 5.3 Å
EM Method: Single-particle
Fitted PDBs: 8uyd
Q-score: 0.147
Risi CM, Belknap B, Atherton J, Coscarella IL, White HD, Bryant Chase P, Pinto JR, Galkin VE
J Mol Biol (2024) 436 pp. 168498-168498 [ Pubmed: 38387550 DOI: doi:10.1016/j.jmb.2024.168498 ]
- Magnesium ion (24 Da, Ligand)
- Troponin t2, cardiac type (33 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tropomyosin alpha-1 chain (32 kDa, Protein from Sus scrofa)
- Troponin c, slow skeletal and cardiac muscles (18 kDa, Protein from Sus scrofa)
- Troponin i, cardiac muscle (24 kDa, Protein from Sus scrofa)
- Actin, alpha cardiac muscle 1 (42 kDa, Protein from Sus scrofa)
- Thin filament troponin core complex (Complex from Sus scrofa)
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 8uz5
Q-score: 0.142
Risi CM, Belknap B, Atherton J, Coscarella IL, White HD, Bryant Chase P, Pinto JR, Galkin VE
J Mol Biol (2024) 436 pp. 168498-168498 [ Pubmed: 38387550 DOI: doi:10.1016/j.jmb.2024.168498 ]
- Magnesium ion (24 Da, Ligand)
- Troponin t2, cardiac type (33 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tropomyosin alpha-1 chain (32 kDa, Protein from Sus scrofa)
- Troponin c, slow skeletal and cardiac muscles (18 kDa, Protein from Sus scrofa)
- Troponin i, cardiac muscle (24 kDa, Protein from Sus scrofa)
- Actin, alpha cardiac muscle 1 (42 kDa, Protein from Sus scrofa)
- Thin filament troponin core complex (Complex from Sus scrofa)
Resolution: 7.1 Å
EM Method: Single-particle
Fitted PDBs: 8uz6
Q-score: 0.148
Risi CM, Belknap B, Atherton J, Coscarella IL, White HD, Bryant Chase P, Pinto JR, Galkin VE
J Mol Biol (2024) 436 pp. 168498-168498 [ Pubmed: 38387550 DOI: doi:10.1016/j.jmb.2024.168498 ]
- Magnesium ion (24 Da, Ligand)
- Troponin t2, cardiac type (33 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tropomyosin alpha-1 chain (32 kDa, Protein from Sus scrofa)
- Troponin c, slow skeletal and cardiac muscles (18 kDa, Protein from Sus scrofa)
- Troponin i, cardiac muscle (24 kDa, Protein from Sus scrofa)
- Actin, alpha cardiac muscle 1 (42 kDa, Protein from Sus scrofa)
- Thin filament troponin core complex (Complex from Sus scrofa)
Resolution: 5.4 Å
EM Method: Single-particle
Fitted PDBs: 8uzx
Q-score: 0.15
Risi CM, Belknap B, Atherton J, Coscarella IL, White HD, Bryant Chase P, Pinto JR, Galkin VE
J Mol Biol (2024) 436 pp. 168498-168498 [ Pubmed: 38387550 DOI: doi:10.1016/j.jmb.2024.168498 ]
- Magnesium ion (24 Da, Ligand)
- Troponin t2, cardiac type (33 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tropomyosin alpha-1 chain (32 kDa, Protein from Sus scrofa)
- Troponin c, slow skeletal and cardiac muscles (18 kDa, Protein from Sus scrofa)
- Troponin i, cardiac muscle (24 kDa, Protein from Sus scrofa)
- Actin, alpha cardiac muscle 1 (42 kDa, Protein from Sus scrofa)
- Calcium ion (40 Da, Ligand)
- Thin filament troponin core complex (Complex from Sus scrofa)