Eukaryotic 80S ribosome with Reh1, eIF5A and A/P site tRNA
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 8ut0
Q-score: 0.473
Musalgaonkar S, Yelland JN, Chitale R, Rao S, Ozadam H, Taylor DW, Cenik C, Johnson AW
Nat Commun (2025) 16 pp. 1278-1278 [ DOI: doi:10.1038/s41467-025-55844-8 Pubmed: 39900920 ]
- P site initiator trna (24 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s31 (8 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s15 (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- Messenger rna (5'-r(p*ap*ap*up*ap*up*gp*ap*ap*ap*a)-3') (3 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s20 (11 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s17-b (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s10-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s2 (23 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s25-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l43-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l7-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l40-a (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l8-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l34-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l1 (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s5 (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s23-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s26-b (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein asc1 (34 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s30-a (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s0-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l10 (25 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6-a (26 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l24-a (7 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s3 (24 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s18-a (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l11-b (19 kDa, Protein from Saccharomyces cerevisiae)
- A site trna (24 kDa, RNA from Saccharomyces cerevisiae)
- Cytoplasmic 60s subunit biogenesis factor reh1 (n-terminal 3xflag tag) (67 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s14-b (13 kDa, Protein from Saccharomyces cerevisiae)
- Eukaryotic 80s ribosome with reh1, eif5a and a/p site trna (Complex from Saccharomyces cerevisiae)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l30 (10 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l25 (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l41-a (3 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l14-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 18s rrna (579 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l42-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l6-b (19 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s1-a (26 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s11-a (16 kDa, Protein from Saccharomyces cerevisiae)
- 4-{(2r)-2-[(1s,3s,5s)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione (281 Da, Ligand)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l13-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s12 (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Translation factor eif5a (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s8-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l22-a (11 kDa, Protein from Saccharomyces cerevisiae)
CryoEM structure of DHS-eIF5A complex structure from Trichomonas vaginalis
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 8qzx
Q-score: 0.465
Wator E, Wilk P, Kochanowski P, Grudnik P
FEBS J (2024) 291 pp. 3856-3869 [ DOI: doi:10.1111/febs.17207 Pubmed: 38923395 ]
- Deoxyhypusine synthase related protein, putative (40 kDa, Protein from Trichomonas vaginalis)
- Eukaryotic translation initiation factor 5a (18 kDa, Protein from Trichomonas vaginalis)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Spermidine (145 Da, Ligand)
- Complex of dhs-eif5a (Complex from Trichomonas vaginalis)
Structure of ribosomal complex bound with Rbg1/Tma46
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 7rr5
Q-score: 0.437
Zeng F, Li X, Pires-Alves M, Chen X, Hawk CW, Jin H
Cell Rep (2021) 37 pp. 109877-109877 [ Pubmed: 34706231 DOI: doi:10.1016/j.celrep.2021.109877 ]
- 40s ribosomal protein s11-b (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s10-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l6 (20 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae)
- Rpl32 isoform 1 (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae)
- Eukaryotic translation initiation factor 5a (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l2-b (27 kDa, Protein from Saccharomyces cerevisiae)
- Rpl29 isoform 1 (6 kDa, Protein from Saccharomyces cerevisiae)
- Ribosome-interacting gtpase 1 (45 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Rpl12a isoform 1 (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae)
- Rps3 isoform 1 (26 kDa, Protein from Saccharomyces cerevisiae)
- Rps20 isoform 1 (13 kDa, Protein from Saccharomyces cerevisiae)
- Rps29a isoform 1 (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s subunit (Complex from Saccharomyces cerevisiae)
- Translation machinery-associated protein 46 (39 kDa, Protein from Saccharomyces cerevisiae)
- Rps26b isoform 1 (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s30 (7 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l13 (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s subunit (Complex from Saccharomyces cerevisiae)
- A-trna (Complex from Saccharomyces cerevisiae)
- Rps25a isoform 1 (12 kDa, Protein from Saccharomyces cerevisiae)
- Rps5p (25 kDa, Protein from Saccharomyces cerevisiae)
- Rps22a isoform 1 (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s17-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Rpl38 isoform 1 (8 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s23 (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae)
- Rps15 isoform 1 (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l18-b (20 kDa, Protein from Saccharomyces cerevisiae)
- Rpl11b isoform 1 (19 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l1 (17 kDa, Protein from Saccharomyces cerevisiae)
- Ribosomal protein l37 (9 kDa, Protein from Saccharomyces cerevisiae)
- Rpl24a isoform 1 (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l23-b (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s18-b (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s0 (28 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s7 (21 kDa, Protein from Saccharomyces cerevisiae)
- Mrna (5 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae)
- Mrna (Complex from Saccharomyces cerevisiae)
- Rbg1/tma46 (Complex from Saccharomyces cerevisiae)
- 60s ribosomal protein l42-a (12 kDa, Protein from Saccharomyces cerevisiae)
- Rps2 isoform 1 (27 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s24 (15 kDa, Protein from Saccharomyces cerevisiae)
- Rpl4a isoform 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s14-b (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae)
- Ribosomal complex with rbg1/tma46 (Complex from Saccharomyces cerevisiae)
- 40s ribosomal protein s4 (29 kDa, Protein from Saccharomyces cerevisiae)
- P-trna (Complex from Saccharomyces cerevisiae)
- Rpl10 isoform 1 (25 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae)
- Aspartic acid (133 Da, Ligand)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae)
- Rpl3 isoform 1 (43 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- P-trna (24 kDa, RNA from Saccharomyces cerevisiae)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l41-a (3 kDa, Protein from Saccharomyces cerevisiae)
- Rps8a isoform 1 (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 18s rrna (579 kDa, RNA from Saccharomyces cerevisiae)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae)
- A-trna (24 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae)
- Eif5a (Complex from Saccharomyces cerevisiae)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Saccharomyces cerevisiae)
- Rpl5 isoform 1 (33 kDa, Protein from Saccharomyces cerevisiae)
- Rpp0 isoform 1 (33 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l27 (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6 (27 kDa, Protein from Saccharomyces cerevisiae)
- Rps1a isoform 1 (28 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Saccharomyces cerevisiae)
- Rps28a isoform 1 (7 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7nrc
Q-score: 0.378
Pochopien AA, Beckert B, Kasvandik S, Berninghausen O, Beckmann R, Tenson T, Wilson DN
PNAS (2021) 118 [ Pubmed: 33790014 DOI: doi:10.1073/pnas.2022756118 ]
- 5s rrna (121-mer) (38 kDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l41-a (3 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l25 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s14-b (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s17-b (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l6-b (19 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l43-a (9 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s10-a (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l11-b (19 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l8-a (25 kDa, Protein from Saccharomyces cerevisiae S288C)
- Structure of the yeast gcn1-bound leading stalled 80s ribosome with bound rbg2, gir2, a- and p-trna and eif5a (Complex from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- Trna (75-mer) (24 kDa, RNA from Saccharomyces cerevisiae S288C)
- Eif5a (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- Gir2 (4 kDa, Protein from Saccharomyces cerevisiae S288C)
- Trna (76-mer) (24 kDa, RNA from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s23-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l14-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l13-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s12 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l42-a (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s5 (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l34-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- Gcn1 (98 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- L1 60s ribosomal protein (17 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s1-a (26 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s15 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s20 (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l7-a (25 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l10 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s0-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l24-a (7 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l30 (10 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- 5.8s rrna (158-mer) (50 kDa, RNA from Saccharomyces cerevisiae S288C)
- 18s rrna (1771-mer) (579 kDa, RNA from Saccharomyces cerevisiae S288C)
- 25s rrna (3184-mer) (1 MDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s18-a (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s3 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s6-a (26 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s9-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s25-a (9 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome-interacting gtpase 2 (40 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s2 (23 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s13 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s11-a (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l40-a (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- Rna (5'-r(p*ap*up*gp*ap*ap*a)-3') (1 kDa, RNA from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s30-a (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s26-b (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l22-a (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s31 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
Yeast 80S ribosome in complex with eIF5A and decoding A-site and P-site tRNAs.
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6tnu
Q-score: 0.463
Buschauer R, Matsuo Y, Sugiyama T, Chen YH, Alhusaini N, Sweet T, Ikeuchi K, Cheng J, Matsuki Y, Nobuta R, Gilmozzi A, Berninghausen O, Tesina P, Becker T, Coller J, Inada T, Beckmann R
Science (2020) 368 [ DOI: doi:10.1126/science.aay6912 Pubmed: 32299921 ]
- Ubiquitin-60s ribosomal protein l40 (6 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-40s ribosomal protein s31 (8 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s15 (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s20 (11 kDa, Protein from Saccharomyces cerevisiae)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s17-b (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae)
- Eukaryotic translation initiation factor 5a-1 (16 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s25-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s10-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s2 (23 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l43-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 18s rrna (579 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s30-a (6 kDa, Protein from Saccharomyces cerevisiae)
- Mrna (1 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l7-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae)
- Spermidine (145 Da, Ligand)
- 60s ribosomal protein l8-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l34-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s8 (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s23-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s26-b (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s0-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l10 (25 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6-a (26 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s3 (24 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l24-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s18-a (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l1 (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l11-b (19 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s14-b (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l30 (10 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l25 (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l41-a (3 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l14-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l42-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l6-b (19 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s1-a (26 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s11-a (16 kDa, Protein from Saccharomyces cerevisiae)
- 4-{(2r)-2-[(1s,3s,5s)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione (281 Da, Ligand)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l13-a (21 kDa, Protein from Saccharomyces cerevisiae)
- P-site trna (24 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s12 (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae)
- Rps5p (22 kDa, Protein from Saccharomyces cerevisiae)
- A-site trna (24 kDa, RNA from Saccharomyces cerevisiae)
- Yeast 80s ribosome in complex with eif5a programed by a-site and p-site trna. (Complex from Saccharomyces cerevisiae)
- 60s ribosomal protein l22-a (11 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM map of a native 80S-ribosome-eIF-5A complex
Resolution: 3.88 Å
EM Method: Single-particle
Fitted PDBs: 5gak
Q-score: -0.012
Schmidt C, Becker T, Heuer A, Braunger K, Shanmuganathan V, Pech M, Berninghausen O, Wilson DN, Beckmann R
Nucleic Acids Res (2016) 44 pp. 1944-1951 [ DOI: doi:10.1093/nar/gkv1517 Pubmed: 26715760 ]
- Ribosome (Complex from Saccharomyces cerevisiae)
- Eif-5a (170 kDa, Protein from Saccharomyces cerevisiae)
- Native 80s ribosome-eif-5a complex from yeast (Sample)