Cryo-EM structure of EBV gH/gL-gp42 in complex with fab 2C1
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 8khr
Q-score: 0.465
Zhao GX, Fang XY, Bu GL, Chen SJ, Sun C, Li T, Xie C, Wang Y, Li SX, Meng N, Feng GK, Zhong Q, Kong XW, Liu Z, Zeng MS
Cell Rep Med (2024) 5 pp. 101573-101573 [ Pubmed: 38776874 DOI: doi:10.1016/j.xcrm.2024.101573 ]
- Soluble gp42 (22 kDa, Protein from Epstein-Barr virus (strain GD1))
- Envelope glycoprotein h (72 kDa, Protein from Epstein-Barr virus (strain GD1))
- Pentamer complex of gh/gl-gp42 with fab 2c1 (Complex from Epstein-Barr virus (strain GD1))
- 2c1 heavy chain (11 kDa, Protein from Homo sapiens)
- Envelope glycoprotein l (11 kDa, Protein from Epstein-Barr virus (strain GD1))
- 2c1 light chain (11 kDa, Protein from Homo sapiens)
Cryo-EM structure of EBV gHgL-gp42 in complex with mAb 10E4 (localized refinement)
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 7yp1
Q-score: 0.523
Hong J, Zhong L, Liu L, Wu Q, Zhang W, Chen K, Wei D, Sun H, Zhou X, Zhang X, Kang YF, Huang Y, Chen J, Wang G, Zhou Y, Chen Y, Feng QS, Yu H, Li S, Zeng MS, Zeng YX, Xu M, Zheng Q, Chen Y, Zhang X, Xia N
Cell Rep Med (2023) 4 pp. 101296-101296 [ Pubmed: 37992686 DOI: doi:10.1016/j.xcrm.2023.101296 ]
- Gh,gl (Complex from Human gammaherpesvirus 4)
- 10e4 (Complex from Oryctolagus cuniculus)
- Ebv gl (8 kDa, Protein from Human gammaherpesvirus 4)
- 10e4 light chain (11 kDa, Protein from Oryctolagus cuniculus)
- Ebv gh (22 kDa, Protein from Human gammaherpesvirus 4)
- 10e4 heavy chain (12 kDa, Protein from Oryctolagus cuniculus)
- Ghgl-gp42 in complex with mab 10e4 (Complex)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7t4q
Q-score: 0.54
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fab 2c12 light chain (25 kDa, Protein from Homo sapiens)
- Fab 2c12 heavy chain (27 kDa, Protein from Homo sapiens)
- Envelope protein ul128 (19 kDa, Protein from Human betaherpesvirus 5)
- Fab 7i13 light chain (25 kDa, Protein from Homo sapiens)
- Envelope glycoprotein l (30 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein ul130 (28 kDa, Protein from Human betaherpesvirus 5)
- Pentameric complex of hcmv proteins gh, gl, ul128, ul130, ul131a bound to fabs of human neutralizing antibodies 2c12, 7i13 and 13h11 (320 kDa, Complex)
- Fab 7i13 heavy chain (27 kDa, Protein from Homo sapiens)
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Envelope protein ul131a (15 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein h (87 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7t4r
Q-score: 0.429
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Thrombomodulin (55 kDa, Protein from Homo sapiens)
- Fab msl-109 heavy chain (27 kDa, Protein from Homo sapiens)
- Complex of 2x hcmv pentamer gh, gl, ul128, ul130, ul131a bound to 1x thbd and 2x fabs of human neutralizing antibodies msl-109 and 13h11 (590 kDa, Complex)
- Envelope protein ul128 (19 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein l (30 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein ul130 (28 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Envelope protein ul131a (15 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein h (87 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
- Fab msl-109 light chain (28 kDa, Protein from Homo sapiens)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7t4s
Q-score: 0.57
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- Fab 8i21 light chain (25 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Envelope protein ul128 (19 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein l (30 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein ul130 (28 kDa, Protein from Human betaherpesvirus 5)
- Fab 8i21 heavy chain (26 kDa, Protein from Homo sapiens)
- Neuropilin-2 (98 kDa, Protein from Homo sapiens)
- Pentameric complex of hcmv proteins gh, gl, ul128, ul130, ul131a bound to neuropilin 2 and fabs of human neutralizing antibodies 8i21 and 13h11 (360 kDa, Complex)
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Envelope protein ul131a (15 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein h (87 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- Hcmv envelope glycoprotein h (Protein from Human betaherpesvirus 5)
- Fab 13h11 heavy chain (Protein from Homo sapiens)
- Hcmv envelope glycoprotein ul128 (Protein from Human betaherpesvirus 5)
- Hcmv envelope glycoprotein ul130 (Protein from Human betaherpesvirus 5)
- Hcmv envelope glycoprotein l (Protein from Human betaherpesvirus 5)
- Fab 13h11 light chain (Protein from Homo sapiens)
- Complex of 2x hcmv pentamer proteins gh, gl, ul128, ul130, ul131a bound to neuropilin 2 and 2x fabs of human neutralizing antibodies 8i21 and 13h11 (720 kDa, Complex)
- Fab 8i21 light chain (Protein from Homo sapiens)
- Hcmv envelope glycoprotein ul131a (Protein from Human betaherpesvirus 5)
- Fab 8i21 heavy chain (Protein from Homo sapiens)
- Neuropilin-2 (Protein from Homo sapiens)
Epstein-Barr Virus glycoproteins, gH/gL/gp42 inserted ferritin nanoparticles
Bu W, Joyce MG, Nguyen H, Banh DV, Aguilar F, Tariq Z, Yap ML, Tsujimura Y, Gillespie RA, Tsybovsky Y, Andrews SF, Narpala SR, McDermott AB, Rossmann MG, Yasutomi Y, Nabel GJ, Kanekiyo M, Cohen JI
Immunity (2019) 50 pp. 1305-1316.e6 [ DOI: doi:10.1016/j.immuni.2019.03.010 Pubmed: 30979688 ]
Epstein-Barr Virus glycoproteins, gH/gL inserted ferritin nanoparticles
Bu W, Joyce MG, Nguyen H, Banh DV, Aguilar F, Tariq Z, Yap ML, Tsujimura Y, Gillespie RA, Tsybovsky Y, Andrews SF, Narpala SR, McDermott AB, Rossmann MG, Yasutomi Y, Nabel GJ, Kanekiyo M, Cohen JI
Immunity (2019) 50 pp. 1305-1316.e6 [ DOI: doi:10.1016/j.immuni.2019.03.010 Pubmed: 30979688 ]
RCT reconstruction of glycoprotein gHgLgO in complex with neutralizing Fab fragments 3G16 and 13H11
Ciferri C, Chandramouli S, Leitner A, Donnarumma D, Cianfrocco MA, Gerrein R, Friedrich K, Aggarwal Y, Palladino G, Aebersold R, Norais N, Settembre EC, Carfi A
PLoS Pathog (2015) 11 pp. e1005230-e1005230 [ Pubmed: 26485028 DOI: doi:10.1371/journal.ppat.1005230 ]
- Glycoprotein gh/gl/go complex bound to 13h11 and 3g16 fab fragment (300 kDa, Sample)
- Glycoprotein o (300 kDa, Protein from Cytomegalovirus)
- Glycoprotein l (300 kDa, Protein from Cytomegalovirus)
- Glycoprotein h (300 kDa, Protein from Cytomegalovirus)
- 13h11 antibody fab fragment (300 kDa, Protein from unidentified)
- 3g16 antibody fab fragment (300 kDa, Protein from unidentified)