Cryo-EM structure of the monomeric SPARSA gRNA-ssDNA complex
Resolution: 2.57 Å
EM Method: Single-particle
Fitted PDBs: 8y7z
Q-score: 0.587
Cui N, Zhang JT, Li Z, Wei XY, Wang J, Jia N
Nat Commun (2024) 15 pp. 8610-8610 [ Pubmed: 39366953 DOI: doi:10.1038/s41467-024-52910-5 ]
- Monomeric sparsa grna-ssdna complex (Complex from Geobacter sulfurreducens)
- Rna (21-mer) (6 kDa, RNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- Piwi domain protein (53 kDa, Protein from Geobacter sulfurreducens)
- Sir2 superfamily protein (66 kDa, Protein from Geobacter sulfurreducens)
- Magnesium ion (24 Da, Ligand)
- Dna (25-mer) (7 kDa, DNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
Cryo-EM structure of the tetrameric SPARSA gRNA-ssDNA complex
Resolution: 3.38 Å
EM Method: Single-particle
Fitted PDBs: 8y80
Q-score: 0.331
Cui N, Zhang JT, Li Z, Wei XY, Wang J, Jia N
Nat Commun (2024) 15 pp. 8610-8610 [ Pubmed: 39366953 DOI: doi:10.1038/s41467-024-52910-5 ]
- Tetrameric sparsa grna-ssdna complex (Complex from Geobacter sulfurreducens)
- Rna (21-mer) (6 kDa, RNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- Piwi domain protein (53 kDa, Protein from Geobacter sulfurreducens)
- Sir2 superfamily protein (66 kDa, Protein from Geobacter sulfurreducens)
- Magnesium ion (24 Da, Ligand)
- Dna (25-mer) (7 kDa, DNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
Cryo-EM structure of monomeric SPARTA gRNA-ssDNA target complex
Resolution: 2.54 Å
EM Method: Single-particle
Fitted PDBs: 8ifk
Q-score: 0.602
Zhang JT, Wei XY, Cui N, Tian R, Jia N
Nat Chem Biol (2024) 20 pp. 503-511 [ Pubmed: 37932528 DOI: doi:10.1038/s41589-023-01479-z ]
- Piwi domain-containing protein (58 kDa, Protein from Thermoflavifilum thermophilum)
- Tir domain-containing protein (53 kDa, Protein from Thermoflavifilum thermophilum)
- Target ssdna (7 kDa, DNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- Crtsparta-grna-tdna ternary complex (Complex from Thermoflavifilum thermophilum)
- Magnesium ion (24 Da, Ligand)
- Guide rna (6 kDa, RNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
Cryo-EM structure of SPARTA gRNA binary complex
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8k34
Q-score: 0.441
Zhang JT, Wei XY, Cui N, Tian R, Jia N
Nat Chem Biol (2024) 20 pp. 503-511 [ Pubmed: 37932528 DOI: doi:10.1038/s41589-023-01479-z ]
- Rna (5'-r(p*ap*ap*ap*cp*gp*gp*cp*up*cp*up*ap*ap*up*cp*up*ap*up*up*ap*gp*u)-3') (6 kDa, RNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- Piwi domain-containing protein (58 kDa, Protein from Thermoflavifilum thermophilum)
- Crtsparta-grna binary complex (Complex from Thermoflavifilum thermophilum)
- Tir domain-containing protein (53 kDa, Protein from Thermoflavifilum thermophilum)
- Magnesium ion (24 Da, Ligand)