Cas12a:gRNA:DNA (Acidaminococcus sp.) with 0 RNA:DNA base pairs, structure 1
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 9cjh
Q-score: 0.296
Soczek KM, Cofsky JC, Tuck OT, Shi H, Doudna JA
Nucleic Acids Res (2025) 53 [ Pubmed: 39698811 DOI: doi:10.1093/nar/gkae1192 ]
- Grna:dna (Complex from synthetic construct)
- Guide rna (25-mer) (13 kDa, RNA from synthetic construct)
- Target dna strand (9 kDa, DNA from synthetic construct)
- Cas12a:grna:dna (acidaminococcus sp.) with 0 rna:dna base pairs, structure 1 (185 kDa, Complex)
- Non-target dna strand (9 kDa, DNA from synthetic construct)
- Cas12a (Complex from Acidaminococcus sp. BV3L6)
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
Cas12a:gRNA:DNA (Acidaminococcus sp.) with 0 RNA:DNA base pairs, structure 2
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 9cji
Q-score: 0.316
Soczek KM, Cofsky JC, Tuck OT, Shi H, Doudna JA
Nucleic Acids Res (2025) 53 [ Pubmed: 39698811 DOI: doi:10.1093/nar/gkae1192 ]
- Grna:dna (Complex from synthetic construct)
- Guide rna (25-mer) (13 kDa, RNA from synthetic construct)
- Non-target dna strand (9 kDa, DNA from synthetic construct)
- Cas12a (Complex from Acidaminococcus sp. BV3L6)
- Target dna strand (9 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
- Cas12a:grna:dna (acidaminococcus sp.) with 0 rna:dna base pairs, structure 2 (185 kDa, Complex)
Cas12a:gRNA:DNA (Acidaminococcus sp.) with 0 RNA:DNA base pairs, structure 3
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9cjj
Q-score: 0.324
Soczek KM, Cofsky JC, Tuck OT, Shi H, Doudna JA
Nucleic Acids Res (2024) [ Pubmed: 39698811 DOI: doi:10.1093/nar/gkae1192 ]
- Grna:dna (Complex from synthetic construct)
- Guide rna (25-mer) (13 kDa, RNA from synthetic construct)
- Target dna strand (9 kDa, DNA from synthetic construct)
- Non-target dna strand (9 kDa, DNA from synthetic construct)
- Cas12a (Complex from Acidaminococcus sp. BV3L6)
- Cas12a:grna:dna (acidaminococcus sp.) with 0 rna:dna base pairs, structure 3 (185 kDa, Complex)
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
Cryo-EM structure of Thermus thermophilus reiterative transcription complex with 11nt oligo-G RNA
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7rdq
Q-score: 0.516
Liu Y, Yu L, Pukhrambam C, Winkelman JT, Firlar E, Kaelber JT, Zhang Y, Nickels BE, Ebright RH
PNAS (2022) 119 [ Pubmed: 35082149 DOI: doi:10.1073/pnas.2115746119 ]
- Reiterative transcription initiation complex with 11nt oligo-g rna (425 kDa, Complex from Thermus thermophilus HB8)
- Magnesium ion (24 Da, Ligand)
- Dna (31-mer) nontemplate strand (10 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (170 kDa, Protein from Thermus thermophilus HB8)
- Dna (31-mer) template strand (10 kDa, DNA from synthetic construct)
- Rna (5'-r(p*gp*gp*gp*gp*gp*gp*gp*gp*gp*gp*g)-3') (3 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus HB8)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus HB8)
- Rna polymerase sigma factor siga (48 kDa, Protein from Thermus thermophilus HB8)