CryoEM reconstruction of hemagglutinin HK68 of Influenza A virus bound to an Affimer reagent
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8pk3
Q-score: 0.346
Debski-Antoniak O, Flynn A, Klebl DP, Rojas Rechy MH, Tiede C, Wilson IA, Muench SP, Tomlinson D, Fontana J
Mbio (2024) 15 pp. e0180424-e0180424 [ DOI: doi:10.1128/mbio.01804-24 Pubmed: 39037231 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hemagglutinin ha1 chain (35 kDa, Protein from Influenza A virus)
- Hemagglutinin ha2 chain (20 kDa, Protein from Influenza A virus)
- Cryoem reconstruction of hemagglutinin hk68 of influenza a virus bound to an affimer reagent (Complex from Influenza A virus)
- Affimer molecule (a31) (10 kDa, Protein from synthetic construct)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8ur5
Q-score: 0.429
Ellis D, Dosey A, Boyoglu-Barnum S, Park YJ, Gillespie R, Syeda H, Hutchinson GB, Tsybovsky Y, Murphy M, Pettie D, Matheson N, Chan S, Ueda G, Fallas JA, Carter L, Graham BS, Veesler D, Kanekiyo M, King NP
Cell Rep (2023) 42 pp. 113552-113552 [ Pubmed: 38096058 DOI: doi:10.1016/j.celrep.2023.113552 ]
- I53_dn5 nanoparticle displaying the trimeric ha heads with heptad domain, th-1heptad-i53_dn5 (local refinement of th-1heptad) (Complex)
- Trimer head ha (Complex from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Trimer head ha,hemagglutinin ha1 chain (44 kDa, Protein from synthetic construct)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8ur7
Q-score: 0.403
Ellis D, Dosey A, Boyoglu-Barnum S, Park YJ, Gillespie R, Syeda H, Hutchinson GB, Tsybovsky Y, Murphy M, Pettie D, Matheson N, Chan S, Ueda G, Fallas JA, Carter L, Graham BS, Veesler D, Kanekiyo M, King NP
Cell Rep (2023) 42 pp. 113552-113552 [ Pubmed: 38096058 DOI: doi:10.1016/j.celrep.2023.113552 ]
- I53_dn5 nanoparticle displaying the trimeric ha heads with heptad domain, th-6heptad-i53_dn5 (Complex)
- Trimer head ha,hemagglutinin ha1 chain (48 kDa, Protein from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Pentamer (Complex from synthetic construct)
- Trimer head ha (Complex from synthetic construct)
Cryo-EM structure of X6 COBRA (H1N1) hemagglutinin bound to CR6261 Fab
Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 8f38
Q-score: 0.615
Nagashima KA, Dzimianski JV, Yang M, Abendroth J, Sautto GA, Ross TM, DuBois RM, Edwards TE, Mousa JJ
Structure (2024) 32 pp. 1079- [ DOI: doi:10.1016/j.str.2024.05.001 Pubmed: 38810648 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cr6261 fab heavy chain (24 kDa, Protein from Homo sapiens)
- Hemagglutinin (63 kDa, Protein from synthetic construct)
- Cr6261 fab light chain (23 kDa, Protein from Homo sapiens)
- Cryo-em structure of x6 cobra (h1n1) hemagglutinin bound to cr6261 fab (450 kDa, Complex)
- Water (18 Da, Ligand)
Cryo-EM structure of designed Influenza HA binder, HA_20, bound to Influenza HA (Strain: Iowa43)
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8sk7
Q-score: 0.528
Watson JL, Juergens D, Bennett NR, Trippe BL, Yim J, Eisenach HE, Ahern W, Borst AJ, Ragotte RJ, Milles LF, Wicky BIM, Hanikel N, Pellock SJ, Courbet A, Sheffler W, Wang J, Venkatesh P, Sappington I, Torres SV, Lauko A, De Bortoli V, Mathieu E, Ovchinnikov S, Barzilay R, Jaakkola TS, DiMaio F, Baek M, Baker D
Nature (2023) 620 pp. 1089-1100 [ DOI: doi:10.1038/s41586-023-06415-8 Pubmed: 37433327 ]
- Hemagglutinin ha1 chain (35 kDa, Protein from Influenza A virus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hemagglutinin (26 kDa, Protein from Influenza A virus)
- Influenza ha (iowa43) bound to rfdiffusion designed minibinder, ha_20 (Complex from Influenza A virus)
- Ha_20 minibinder (rfdiffusion-designed) (7 kDa, Protein from synthetic construct)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7kna
Q-score: 0.499
Boyoglu-Barnum S, Ellis D, Gillespie RA, Hutchinson GB, Park YJ, Moin SM, Acton OJ, Ravichandran R, Murphy M, Pettie D, Matheson N, Carter L, Creanga A, Watson MJ, Kephart S, Ataca S, Vaile JR, Ueda G, Crank MC, Stewart L, Lee KK, Guttman M, Baker D, Mascola JR, Veesler D, Graham BS, King NP, Kanekiyo M
Nature (2021) 592 pp. 623-628 [ Pubmed: 33762730 DOI: doi:10.1038/s41586-021-03365-x ]
3D reconstruction of a ferritin-based nanoparticle displaying H1 Hemagglutinin stem epitopes
Yassine HM, Boyington JC, McTamney PM, Wei CJ, Kanekiyo M, Kong WP, Gallagher JR, Wang L, Zhang Y, Joyce MG, Lingwood D, Moin SM, Andersen H, Okuno Y, Rao SS, Harris AK, Kwong PD, Mascola JR, Nabel GJ, Graham BS
Nat Med (2015) 21 pp. 1065-1070 [ DOI: doi:10.1038/nm.3927 Pubmed: 26301691 ]