Cenp-A nucleosome in complex with Cenp-C
Resolution: 3.35 Å
EM Method: Single-particle
Fitted PDBs: 7on1
Q-score: 0.47
Yan K, Yang J, Zhang Z, Barford D
To Be Published
- Dna (152-mer), dna (123-mer) (Complex from Escherichia coli)
- Histones (Complex from Saccharomyces cerevisiae)
- Kinetochore subunit (Complex from Saccharomyces cerevisiae)
- Cenp-a nucleosome in complex with cenp-c (Complex)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae)
- Bj4_g0006610.mrna.1.cds.1 (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna (152-mer) (45 kDa, DNA from Escherichia coli)
- Dna (123-mer) (38 kDa, DNA from Escherichia coli)
- Histone h2a (14 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2b (14 kDa, Protein from Saccharomyces cerevisiae)
- Bj4_g0007000.mrna.1.cds.1 (27 kDa, Protein from Saccharomyces cerevisiae)
Active state Dot1 bound to the unacetylated H4 nucleosome
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7k6p
Q-score: 0.473
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ DOI: doi:10.1126/science.abc6663 Pubmed: 33479126 ]
- Dna (146-mer) (Complex from synthetic construct)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (46 kDa, Protein from Saccharomyces cerevisiae)
- S-adenosylmethionine (398 Da, Ligand)
- Dna (146-mer) (44 kDa, DNA from synthetic construct)
- Dna (146-mer) (45 kDa, DNA from synthetic construct)
- Histone h2a type 1 (11 kDa, Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Histone h3.2 (11 kDa, Protein from Xenopus laevis)
- Histone h4 (10 kDa, Protein from Xenopus laevis)
- Active state dot1 bound to the unacetylated h4 nucleosome (Complex from Saccharomyces cerevisiae)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (10 kDa, Protein from Xenopus laevis)
- Polyubiquitin-b (Complex from Homo sapiens)
Active state Dot1 bound to the H4K16ac nucleosome
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7k6q
Q-score: 0.489
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ DOI: doi:10.1126/science.abc6663 Pubmed: 33479126 ]
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (46 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Dna (146-mer) (44 kDa, DNA from synthetic construct)
- Dna (146-mer) (45 kDa, DNA from synthetic construct)
- Histone h2a type 1 (11 kDa, Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Histone h3.2 (11 kDa, Protein from Xenopus laevis)
- Histone h4 (10 kDa, Protein from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
- Dna (146-mer) (Complex from Synthetic construct)
- Active state dot1 bound to the h4k16ac nucleosome (Complex from Saccharomyces cerevisiae)
- Histone h2b 1.1 (10 kDa, Protein from Xenopus laevis)
- Polyubiquitin-b (Complex from Homo sapiens)
Noncatalytic conformation Dot1 bound to the unacetylated H4 nucleosome
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ DOI: doi:10.1126/science.abc6663 Pubmed: 33479126 ]
- Histone h3.2 (Protein from Xenopus lenduensis)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Dna (146-mer) (DNA from synthetic construct)
- Polyubiquitin-b (Protein from Homo sapiens)
- Noncatalytic conformation dot1 bound to the unacetylated h4 nucleosome (Complex from Saccharomyces cerevisiae)
- Histone h4 (Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Protein from Saccharomyces cerevisiae)
- Dna (146-mer) (Complex from Synthetic)
- Polyubiquitin-b (Complex from Homo sap)
Active state Dot1 bound to the H4K16ac nucleosome, Ubiquitin focused map
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ DOI: doi:10.1126/science.abc6663 Pubmed: 33479126 ]
- Active state dot1 bound to the h4k16 acetylated nucleosome (Complex from Saccharomyces cerevisiae)
- Polyubiquitin-b (Protein from Xenopus laevis)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Histone h3.2 (Protein from Xenopus laevis)
- Dna (146-mer) (DNA from synthetic construct)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Protein from Xenopus laevis)
- Histone h4 (Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Dna (146-mer) (Complex from Synthetic)
- Polyubiquitin-b (Complex from Homo sap)
Active state Dot1 bound to the unacetylated H4 nucleosome, Ubiquitin focused map
Valencia-Sanchez MI, De Ioannes P, Wang M, Truong DM, Lee R, Armache JP, Boeke JD, Armache KJ
Science (2021) 371 [ DOI: doi:10.1126/science.abc6663 Pubmed: 33479126 ]
- Polyubiquitin-b (Protein from Xenopus laevis)
- Histone h2a type 1 (Protein from Xenopus laevis)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Histone h3.2 (Protein from Xenopus laevis)
- Dna (146-mer) (DNA from synthetic construct)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Protein from Xenopus laevis)
- Histone h4 (Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (Complex from Saccharomyces cerevisiae)
- Dna (146-mer) (Complex from Synthetic)
- Active state dot1 bound to the unacetylated h4 nucleosome (Complex from Saccharomyces cerevisiae)
- Polyubiquitin-b (Complex from Homo sap)
Histone H3 recognition by nucleosome-bound PRC2 subunit EZH2.
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 7at8
Q-score: 0.29
Finogenova K, Bonnet J, Poepsel S, Schafer IB, Finkl K, Schmid K, Litz C, Strauss M, Benda C, Muller J
eLife (2020) 9 [ Pubmed: 33211010 DOI: doi:10.7554/eLife.61964 ]
- Suz12 and ezh2 (Complex from Homo sapiens)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Zinc ion (65 Da, Ligand)
- Polycomb protein suz12 (83 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- Histones (Complex from Xenopus laevis)
- Isoform 2 of histone-lysine n-methyltransferase ezh2,isoform 2 of histone-lysine n-methyltransferase ezh2,histone-lysine n-methyltransferase ezh2,isoform 2 of histone-lysine n-methyltransferase ezh2,isoform 2 of histone-lysine n-methyltransferase ezh2 (87 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Em map of ezh2 on nucleosome obtained from signal particle subtraction and focused refinement on phf1-prc2:dinucleosome map. (Complex)
- Widom601 dna plus linker (47 kDa, DNA from synthetic construct)
- Widom601 dna plus linker (48 kDa, DNA from synthetic construct)
Cryo-EM map of PHF1-PRC2 on a heterodimeric dinucleosome.
Finogenova K, Bonnet J, Poepsel S, Schafer IB, Finkl K, Schmid K, Litz C, Strauss M, Benda C, Muller J
eLife (2020) 9 [ Pubmed: 33211010 DOI: doi:10.7554/eLife.61964 ]
- Histone h2b (Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase ezh2 (Protein from Homo sapiens)
- Histone-lysine n-methyltransferase ezh2, polycomb protein eed, polycomb protein suz12 (Complex from Homo sapiens)
- Polycomb protein eed (Protein from Homo sapiens)
- Histone h3.2 (Protein from Xenopus laevis)
- Cryo-em map of phf1-prc2 on a heterodimeric dinucleosome (Complex)
- Polycomb protein suz12 (Protein from Homo sapiens)
- Histone h4 (Protein from Xenopus laevis)
- Histones (Complex from Xenopus laevis)
- Widom601 dna + linker (Complex from synthetic construct)
- Widom601 dna + linker (RNA from synthetic construct)
- Histone h2a (Protein from Xenopus laevis)
Structure of human Sox11 transcription factor in complex with a nucleosome
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6t7a
Q-score: 0.37
Dodonova SO, Zhu F, Dienemann C, Taipale J, Cramer P
Nature (2020) 580 pp. 669-672 [ DOI: doi:10.1038/s41586-020-2195-y Pubmed: 32350470 ]
- Transcription factor sox-11 (12 kDa, Protein from Homo sapiens)
- Structure of human sox11 transcription factor in complex with a nucleosome (218 kDa, Complex)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (147-mer) (45 kDa, DNA from synthetic construct)
- Dna (147-mer) (45 kDa, DNA from synthetic construct)
- Histone h2a type 1-b/e (16 kDa, Protein from Homo sapiens)
- Histone h2b type 1-k (13 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Sox11 transcription factor, histones (Complex from Homo sapiens)
- Histone h3.2 (15 kDa, Protein from Homo sapiens)
Structure of two copies of human Sox11 transcription factor in complex with a nucleosome
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6t7c
Q-score: 0.355
Dodonova SO, Zhu F, Dienemann C, Taipale J, Cramer P
Nature (2020) 580 pp. 669-672 [ DOI: doi:10.1038/s41586-020-2195-y Pubmed: 32350470 ]
- Histones and sox11 (Complex from Homo sapiens)
- Transcription factor sox-11 (12 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Structure of two copies of human sox11 transcription factor in complex with a nucleosome (229 kDa, Complex)
- Dna (147-mer) (45 kDa, DNA from synthetic construct)
- Dna (147-mer) (45 kDa, DNA from synthetic construct)
- Histone h2a type 1-b/e (16 kDa, Protein from Homo sapiens)
- Histone h2b type 1-k (13 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Histone h3.2 (15 kDa, Protein from Homo sapiens)