Cryo-EM structure of histamine H2 receptor in complex with histamine and miniGs
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8yn3
Q-score: 0.623
Zhang X, Liu G, Zhong YN, Zhang R, Yang CC, Niu C, Pu X, Sun J, Zhang T, Yang L, Zhang C, Li X, Shen X, Xiao P, Sun JP, Gong W
Nat Commun (2024) 15 pp. 8296-8296 [ Pubmed: 39333117 DOI: doi:10.1038/s41467-024-52585-y ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody nb35 (15 kDa, Protein from synthetic construct)
- Histamine-h2r-minigs-nb35 complex (Complex from Homo sapiens)
- Histamine h2 receptor (62 kDa, Protein from Homo sapiens)
- Histamine (111 Da, Ligand)
- Engineered guanine nucleotide,binding protein g(s) subunit alpha (29 kDa, Protein from synthetic construct)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
A Cryo-EM structure of Bitter taste receptor TAS2R14 with Ggust
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 9iix
Q-score: 0.4
Tao L, Wang D, Yuan Q, Zhao F, Zhang Y, Du T, Shen S, Xu HE, Li Y, Yang D, Duan J
Cell Res (2024) 34 pp. 735-738 [ Pubmed: 38969802 DOI: doi:10.1038/s41422-024-00995-4 ]
- Tas2r14 with ggust complex (Complex from Homo sapiens)
- 4-methyl-n-[(2m)-2-(1h-tetrazol-5-yl)phenyl]-6-(trifluoromethyl)pyrimidin-2-amine (321 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein mini g(s) subunit alpha-1 (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Taste receptor type 2 member 14 (36 kDa, Protein from Homo sapiens)
Structure 2 of human class T GPCR TAS2R14-miniGs/gust complex with Flufenamic acid.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8xqr
Q-score: 0.386
Hu X, Ao W, Gao M, Wu L, Pei Y, Liu S, Wu Y, Zhao F, Sun Q, Liu J, Jiang L, Wang X, Li Y, Tan Q, Cheng J, Yang F, Yang C, Sun J, Hua T, Liu ZJ
Nature (2024) 631 pp. 459-466 [ DOI: doi:10.1038/s41586-024-07569-9 Pubmed: 38776963 ]
- Exo-alpha-sialidase,taste receptor type 2 member 14,lgbit (110 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (14 kDa, Protein from Homo sapiens)
- Structure 2 of human class t gpcr tas2r14-minigs/gust complex with flufenamic acid. (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- 2-[[3-(trifluoromethyl)phenyl]amino] benzoic acid (281 Da, Ligand)
Cryo-EM structure of LPS-bound GPR174 in complex with Gs protein
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7xv3
Q-score: 0.579
Liang J, Inoue A, Ikuta T, Xia R, Wang N, Kawakami K, Xu Z, Qian Y, Zhu X, Zhang A, Guo C, Huang Z, He Y
Nat Commun (2023) 14 pp. 1012-1012 [ DOI: doi:10.1038/s41467-023-36575-0 Pubmed: 36823105 ]
- Water (18 Da, Ligand)
- (2~{s})-2-$l^{4}-azanyl-3-[[(2~{r})-3-octadecanoyloxy-2-oxidanyl-propoxy]-oxidanyl-oxidanylidene-$l^{6}-phosphanyl]oxy-propanoic acid (497 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Engineered g protein subunit s (mini-gs) (41 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 174 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Lama glama)
- Gpr174/gs complex (Complex from Homo sapiens)
Cryo-EM structure of the fenoldopam-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7x2c
Q-score: 0.465
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ DOI: doi:10.1038/s41467-022-30929-w Pubmed: 35676276 ]
- Gbeta-1gama-2 (Complex from Homo sapiens)
- Nanobody35 (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- The fenoldopam-bound d1r-minigs (Complex)
- Nanobody35 (17 kDa, Protein from synthetic construct)
- Cryo-em structure of the fenoldopam-bound d1r-minigs complex (Complex from Homo sapiens)
- (1r)-6-chloranyl-1-(4-hydroxyphenyl)-2,3,4,5-tetrahydro-1h-3-benzazepine-7,8-diol (305 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
Cryo-EM structure of dopamine receptor 1 and mini-Gs complex with dopamine bound
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f0t
Q-score: 0.498
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ Pubmed: 35687690 DOI: doi:10.1126/sciadv.abo4158 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Nanobody35 (17 kDa, Protein from synthetic construct)
- Cryo-em structure of dopamine receptor 1 and mini-gs complex with dopamine bound (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
Cryo-EM structure of the GDP-bound dopamine receptor 1 and mini-Gs complex with Nb35
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 7f1o
Q-score: 0.506
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ Pubmed: 35687690 DOI: doi:10.1126/sciadv.abo4158 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gdp-bound dopamine receptor 1 and mini-gs complex with nb35 (Complex from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
Cryo-EM structure of the GDP-bound dopamine receptor 1 and mini-Gs complex without Nb35
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 7f1z
Q-score: 0.46
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ Pubmed: 35687690 DOI: doi:10.1126/sciadv.abo4158 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gdp-bound dopamine receptor 1 and mini-gs complex without nb35 (Complex from Homo sapiens)
- L-dopamine (153 Da, Ligand)
Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-Gs complex with Nb35
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 7f23
Q-score: 0.456
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ Pubmed: 35687690 DOI: doi:10.1126/sciadv.abo4158 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gtp-bound dopamine receptor 1 and mini-gs complex with nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-Gs complex without Nb35
Resolution: 4.16 Å
EM Method: Single-particle
Fitted PDBs: 7f24
Q-score: 0.364
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ Pubmed: 35687690 DOI: doi:10.1126/sciadv.abo4158 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gtp-bound dopamine receptor 1 and mini-gs complex without nb35 (Complex from Homo sapiens)