Cryo-EM structure of histamine H2 receptor in complex with histamine and miniGs
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8yn3
Q-score: 0.623
Zhang X, Liu G, Zhong YN, Zhang R, Yang CC, Niu C, Pu X, Sun J, Zhang T, Yang L, Zhang C, Li X, Shen X, Xiao P, Sun JP, Gong W
Nat Commun (2024) 15 pp. 8296-8296 [ DOI: doi:10.1038/s41467-024-52585-y Pubmed: 39333117 ]
- Engineered guanine nucleotide,binding protein g(s) subunit alpha (29 kDa, Protein from synthetic construct)
- Histamine h2 receptor (62 kDa, Protein from Homo sapiens)
- Nanobody nb35 (15 kDa, Protein from synthetic construct)
- Histamine (111 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Histamine-h2r-minigs-nb35 complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Structure 2 of human class T GPCR TAS2R14-miniGs/gust complex with Flufenamic acid.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8xqr
Q-score: 0.386
Hu X, Ao W, Gao M, Wu L, Pei Y, Liu S, Wu Y, Zhao F, Sun Q, Liu J, Jiang L, Wang X, Li Y, Tan Q, Cheng J, Yang F, Yang C, Sun J, Hua T, Liu ZJ
Nature (2024) 631 pp. 459-466 [ Pubmed: 38776963 DOI: doi:10.1038/s41586-024-07569-9 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Structure 2 of human class t gpcr tas2r14-minigs/gust complex with flufenamic acid. (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Exo-alpha-sialidase,taste receptor type 2 member 14,lgbit (110 kDa, Protein from synthetic construct)
- 2-[[3-(trifluoromethyl)phenyl]amino] benzoic acid (281 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (14 kDa, Protein from Homo sapiens)
Cholecystokinin 1 receptor (CCK1R) Y140A mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Dong M, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
PLoS Biol (2024) 22 pp. e3002673-e3002673 [ Pubmed: 39083706 DOI: doi:10.1371/journal.pbio.3002673 ]
- Amino group (16 Da, Ligand)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Complex of cck1r (y140a mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Dong M, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
PLoS Biol (2024) 22 pp. e3002673-e3002673 [ Pubmed: 39083706 DOI: doi:10.1371/journal.pbio.3002673 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (29 kDa, Protein from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Complex of cck1r (sterol 7m mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GPR174-Gs complex bound to endogenous lysoPS
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 8kh5
Q-score: 0.514
Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S
Nat Commun (2023) 14 pp. 5901-5901 [ DOI: doi:10.1038/s41467-023-41654-3 Pubmed: 37737235 ]
- Gpr174 (Complex from Homo sapiens)
- Probable g-protein coupled receptor 174 (39 kDa, Protein from Homo sapiens)
- Nb35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- O-{hydroxy[((2r)-2-hydroxy-3-{[(1s)-1-hydroxypentadecyl]oxy}propyl)oxy]phosphoryl}-l-serine (485 Da, Ligand)
- Gs complex (Complex from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Cryo-em structure of the gpr174 and mini-gs complex with nb35 (Complex)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the GPR61-Gs complex
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 8kgk
Q-score: 0.469
Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S
Nat Commun (2023) 14 pp. 5901-5901 [ DOI: doi:10.1038/s41467-023-41654-3 Pubmed: 37737235 ]
- Nb35 (Complex from Lama glama)
- Gpr61 and mini-gs (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gpr61 and mini-gs complex with nb35 (Complex)
- Guanine nucleotide-binding protein g(olf) subunit alpha,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- G-protein coupled receptor 61 (42 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GPR161-Gs complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8kh4
Q-score: 0.48
Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S
Nat Commun (2023) 14 pp. 5901-5901 [ DOI: doi:10.1038/s41467-023-41654-3 Pubmed: 37737235 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 161 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(olf) subunit alpha,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gpr161 and mini-gs complex with nb35 (Complex from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of LPS-bound GPR174 in complex with Gs protein
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7xv3
Q-score: 0.579
Liang J, Inoue A, Ikuta T, Xia R, Wang N, Kawakami K, Xu Z, Qian Y, Zhu X, Zhang A, Guo C, Huang Z, He Y
Nat Commun (2023) 14 pp. 1012-1012 [ DOI: doi:10.1038/s41467-023-36575-0 Pubmed: 36823105 ]
- Probable g-protein coupled receptor 174 (38 kDa, Protein from Homo sapiens)
- (2~{s})-2-$l^{4}-azanyl-3-[[(2~{r})-3-octadecanoyloxy-2-oxidanyl-propoxy]-oxidanyl-oxidanylidene-$l^{6}-phosphanyl]oxy-propanoic acid (497 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gpr174/gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
- Engineered g protein subunit s (mini-gs) (41 kDa, Protein from Homo sapiens)
Structure of A2BR bound to synthetic agonists BAY 60-6583
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8hdo
Q-score: 0.497
Cai H, Xu Y, Guo S, He X, Sun J, Li X, Li C, Yin W, Cheng X, Jiang H, Xu HE, Xie X, Jiang Y
Cell Discov (2022) 8 pp. 140-140 [ Pubmed: 36575181 DOI: doi:10.1038/s41421-022-00503-1 ]
- Nanobody 35 (13 kDa, Protein from Camelus bactrianus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- A2br-g protein-bay 60-6583 complex (160 kDa, Complex from Homo sapiens)
- Adenosine a2b receptor (36 kDa, Protein from Homo sapiens)
- 2-[6-azanyl-3,5-dicyano-4-[4-(cyclopropylmethoxy)phenyl]pyridin-2-yl]sulfanylethanamide (379 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Chimeric minigs (29 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Structure of A2BR bound to endogenous agonists adenosine
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8hdp
Q-score: 0.428
Cai H, Xu Y, Guo S, He X, Sun J, Li X, Li C, Yin W, Cheng X, Jiang H, Xu HE, Xie X, Jiang Y
Cell Discov (2022) 8 pp. 140-140 [ Pubmed: 36575181 DOI: doi:10.1038/s41421-022-00503-1 ]
- Nanobody 35 (13 kDa, Protein from Camelus bactrianus)
- A2br-g protein-adenosine complex (160 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Adenosine a2b receptor (36 kDa, Protein from Homo sapiens)
- Adenosine (267 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Chimeric minigs (29 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)