Cryo-EM structure of DHEA-ADGRG2-FL-Gs complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7xke
Q-score: 0.532
Lin H, Xiao P, Bu RQ, Guo S, Yang Z, Yuan D, Zhu ZL, Zhang CX, He QT, Zhang C, Ping YQ, Zhao RJ, Ma CS, Liu CH, Zhang XN, Jiang D, Huang S, Xi YT, Zhang DL, Xue CY, Yang BS, Li JY, Lin HC, Zeng XH, Zhao H, Xu WM, Yi F, Liu Z, Sun JP, Yu X
Nat Chem Biol (2022) 18 pp. 1196-1203 [ Pubmed: 35982227 DOI: doi:10.1038/s41589-022-01084-6 ]
- Nb35 (13 kDa, Protein from Camelus bactrianus)
- Adhesion g-protein coupled receptor g2 (98 kDa, Protein from Mus musculus)
- 3-beta-hydroxy-5-androsten-17-one (288 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor g2 (220 kDa, Complex from Mus musculus)
- Water (18 Da, Ligand)
- Mini-gs (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the fenoldopam-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7x2c
Q-score: 0.465
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ DOI: doi:10.1038/s41467-022-30929-w Pubmed: 35676276 ]
- Nanobody35 (17 kDa, Protein from synthetic construct)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- The fenoldopam-bound d1r-minigs (Complex)
- (1r)-6-chloranyl-1-(4-hydroxyphenyl)-2,3,4,5-tetrahydro-1h-3-benzazepine-7,8-diol (305 Da, Ligand)
- Cryo-em structure of the fenoldopam-bound d1r-minigs complex (Complex from Homo sapiens)
- Nanobody35 (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Gbeta-1gama-2 (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the tavapadon-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7x2d
Q-score: 0.495
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ DOI: doi:10.1038/s41467-022-30929-w Pubmed: 35676276 ]
- Nanobody35 (17 kDa, Protein from synthetic construct)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cryo-em structure of the tavapadon-bound d1r-minigs complex (Complex from Homo sapiens)
- Nanobody35 (Complex)
- 1,5-dimethyl-6-[2-methyl-4-[3-(trifluoromethyl)pyridin-2-yl]oxy-phenyl]pyrimidine-2,4-dione (391 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Tavapadon-bound d1r-minigs (Complex)
Cryo-EM structure of the dopamine and LY3154207-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7x2f
Q-score: 0.471
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ DOI: doi:10.1038/s41467-022-30929-w Pubmed: 35676276 ]
- Nanobody35 (17 kDa, Protein from synthetic construct)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Nanobody35 (Complex)
- 2-[2,6-bis(chloranyl)phenyl]-1-[(1s,3r)-3-(hydroxymethyl)-1-methyl-5-(3-methyl-3-oxidanyl-butyl)-3,4-dihydro-1h-isoquinolin-2-yl]ethanone (450 Da, Ligand)
- Dopamine-ly3154207-bound d1r-minigs (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Cryo-em structure of the dopamine-ly3154207-bound d1r-minigs complexthe (Complex from Homo sapiens)
Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7wui
Q-score: 0.418
Xiao P, Guo S, Wen X, He QT, Lin H, Huang SM, Gou L, Zhang C, Yang Z, Zhong YN, Yang CC, Li Y, Gong Z, Tao XN, Yang ZS, Lu Y, Li SL, He JY, Wang C, Zhang L, Kong L, Sun JP, Yu X
Nature (2022) 604 pp. 771-778 [ DOI: doi:10.1038/s41586-022-04590-8 Pubmed: 35418677 ]
- Ip15 (Complex)
- Nanobody-35 (13 kDa, Protein from Lama glama)
- Adhesion g-protein coupled receptor g2 (Complex from Mus musculus)
- Ip15 (1 kDa, Protein from synthetic construct)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Cryo-em structure of the adgrg2-fl-ip15-gs complex (253 MDa, Complex)
- Gs (Complex from Homo sapiens)
- Scfv16 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor g2,mcherry (126 kDa, Protein from Mus musculus)
- Nanobody-35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Mini-gs (41 kDa, Protein from Homo sapiens)
Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7wuj
Q-score: 0.474
Xiao P, Guo S, Wen X, He QT, Lin H, Huang SM, Gou L, Zhang C, Yang Z, Zhong YN, Yang CC, Li Y, Gong Z, Tao XN, Yang ZS, Lu Y, Li SL, He JY, Wang C, Zhang L, Kong L, Sun JP, Yu X
Nature (2022) 604 pp. 771-778 [ DOI: doi:10.1038/s41586-022-04590-8 Pubmed: 35418677 ]
- Nanobody-35 (13 kDa, Protein from Lama glama)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Cryo-em structure of the adgr4-beta-gs complex (Complex)
- Adhesion g-protein coupled receptor g4,uncharacterized protein (71 kDa, Protein from Psychromonas sp. B3M02)
- Scfv16 (Complex from synthetic construct)
- Gs (Complex from Homo sapiens)
- Nanobody-35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mini-gs (41 kDa, Protein from Homo sapiens)