Human proton sensing receptor GPR65 in complex with miniGs - focused map of 7TM
Howard MK, Hoppe N, Huang XP, Mitrovic D, Billesbolle CB, Macdonald CB, Mehrotra E, Rockefeller Grimes P, Trinidad DD, Delemotte L, English JG, Coyote-Maestas W, Manglik A
Cell (2025) 188 pp. 671-687.e20 [ DOI: doi:10.1016/j.cell.2024.11.036 Pubmed: 39753132 ]
- Minigs (alpha and beta subunits) (Complex from Homo sapiens)
- Gpr65 receptor in complex with minigs (Complex)
- Nanobody 35 (Complex from Lama glama)
- Gpr65 receptor (Complex from Homo sapiens)
Human proton sensing receptor GPR4 in complex with miniGs
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 9bip
Q-score: 0.567
Howard MK, Hoppe N, Huang XP, Mitrovic D, Billesbolle CB, Macdonald CB, Mehrotra E, Rockefeller Grimes P, Trinidad DD, Delemotte L, English JG, Coyote-Maestas W, Manglik A
Cell (2025) 188 pp. 671-687.e20 [ Pubmed: 39753132 DOI: doi:10.1016/j.cell.2024.11.036 ]
- Minigs (30 kDa, Protein from Homo sapiens)
- Complex of gpr4 bound to gs heterotrimer (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Nb35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 4 (42 kDa, Protein from Homo sapiens)
Consensus olfactory receptor consOR51 in complex with mini-Gs trimeric protein
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8uxv
Q-score: 0.544
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08126-0 Pubmed: 39478229 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Consor51 (36 kDa, Protein from synthetic construct)
- Minigs399 (30 kDa, Protein from Homo sapiens)
- Consor51-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
Consensus olfactory receptor consOR1 bound to L-menthol and in complex with mini-Gs trimeric protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8uxy
Q-score: 0.402
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08126-0 Pubmed: 39478229 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- (1r,2s,5r)-5-methyl-2-(propan-2-yl)cyclohexan-1-ol (156 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Consor1 (35 kDa, Protein from synthetic construct)
- Gnas complex locus (30 kDa, Protein from Homo sapiens)
- Consor51-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
Consensus olfactory receptor consOR2 bound to S-carvone and in complex with mini-Gs trimeric protein
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8uy0
Q-score: 0.489
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08126-0 Pubmed: 39478229 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Consor2-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
- Consor2 (35 kDa, Protein from synthetic construct)
- Minigs399 (30 kDa, Protein from Homo sapiens)
- (5s)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one (150 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8uyq
Q-score: 0.262
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08126-0 Pubmed: 39478229 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Consor4-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- (2r)-2-methyl-1,3-thiazolidine (103 Da, Ligand)
- Consor4 (36 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gnas complex locus (30 kDa, Protein from Homo sapiens)
Cryo-EM structure of histamine H2 receptor in complex with histamine and miniGs
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8yn3
Q-score: 0.623
Zhang X, Liu G, Zhong YN, Zhang R, Yang CC, Niu C, Pu X, Sun J, Zhang T, Yang L, Zhang C, Li X, Shen X, Xiao P, Sun JP, Gong W
Nat Commun (2024) 15 pp. 8296-8296 [ Pubmed: 39333117 DOI: doi:10.1038/s41467-024-52585-y ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody nb35 (15 kDa, Protein from synthetic construct)
- Histamine-h2r-minigs-nb35 complex (Complex from Homo sapiens)
- Histamine h2 receptor (62 kDa, Protein from Homo sapiens)
- Histamine (111 Da, Ligand)
- Engineered guanine nucleotide,binding protein g(s) subunit alpha (29 kDa, Protein from synthetic construct)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
A Cryo-EM structure of Bitter taste receptor TAS2R14 with Ggust
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 9iix
Q-score: 0.4
Tao L, Wang D, Yuan Q, Zhao F, Zhang Y, Du T, Shen S, Xu HE, Li Y, Yang D, Duan J
Cell Res (2024) 34 pp. 735-738 [ Pubmed: 38969802 DOI: doi:10.1038/s41422-024-00995-4 ]
- Tas2r14 with ggust complex (Complex from Homo sapiens)
- 4-methyl-n-[(2m)-2-(1h-tetrazol-5-yl)phenyl]-6-(trifluoromethyl)pyrimidin-2-amine (321 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein mini g(s) subunit alpha-1 (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Taste receptor type 2 member 14 (36 kDa, Protein from Homo sapiens)
Structure 2 of human class T GPCR TAS2R14-miniGs/gust complex with Flufenamic acid.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8xqr
Q-score: 0.386
Hu X, Ao W, Gao M, Wu L, Pei Y, Liu S, Wu Y, Zhao F, Sun Q, Liu J, Jiang L, Wang X, Li Y, Tan Q, Cheng J, Yang F, Yang C, Sun J, Hua T, Liu ZJ
Nature (2024) 631 pp. 459-466 [ DOI: doi:10.1038/s41586-024-07569-9 Pubmed: 38776963 ]
- Exo-alpha-sialidase,taste receptor type 2 member 14,lgbit (110 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (14 kDa, Protein from Homo sapiens)
- Structure 2 of human class t gpcr tas2r14-minigs/gust complex with flufenamic acid. (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- 2-[[3-(trifluoromethyl)phenyl]amino] benzoic acid (281 Da, Ligand)
Cryo-EM structure of LPS-bound GPR174 in complex with Gs protein
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7xv3
Q-score: 0.579
Liang J, Inoue A, Ikuta T, Xia R, Wang N, Kawakami K, Xu Z, Qian Y, Zhu X, Zhang A, Guo C, Huang Z, He Y
Nat Commun (2023) 14 pp. 1012-1012 [ DOI: doi:10.1038/s41467-023-36575-0 Pubmed: 36823105 ]
- Water (18 Da, Ligand)
- (2~{s})-2-$l^{4}-azanyl-3-[[(2~{r})-3-octadecanoyloxy-2-oxidanyl-propoxy]-oxidanyl-oxidanylidene-$l^{6}-phosphanyl]oxy-propanoic acid (497 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Engineered g protein subunit s (mini-gs) (41 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 174 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Lama glama)
- Gpr174/gs complex (Complex from Homo sapiens)