Nat Commun (2025) 16 pp. 782-782 [ Pubmed: 39824870 DOI: doi:10.1038/s41467-025-56143-y ]
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae)
- Cell division cycle protein cdt1 (68 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 1 (108 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Orc-cdt1-mcm complex (1 MDa, Complex from Saccharomyces cerevisiae)
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna (42-mer) (12 kDa, DNA from synthetic construct)
OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm2. Composite map.
Nat Commun (2025) 16 pp. 782-782 [ Pubmed: 39824870 DOI: doi:10.1038/s41467-025-56143-y ]
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae)
- Cell division cycle protein cdt1 (68 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 1 (108 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Orc-cdt1-mcm complex (1 MDa, Complex from Saccharomyces cerevisiae)
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna (42-mer) (12 kDa, DNA from synthetic construct)
OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm5: Conformer 1
Nat Commun (2025) 16 pp. 782-782 [ Pubmed: 39824870 DOI: doi:10.1038/s41467-025-56143-y ]
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae)
- Cell division cycle protein cdt1 (68 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 2 (64 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 1 (108 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae)
- Dna (12 kDa, DNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Orc-cdt1-mcm complex (1 MDa, Complex from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8rif
Q-score: 0.516
Miller TCR, Lim CT, Diffley JFX, Costa A
To Be Published
- Magnesium ion (24 Da, Ligand)
- Mcm-double hexamer loaded onto double-stranded dna (1 MDa, Complex from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna (53-mer) (16 kDa, DNA from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna (53-mer) (16 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of an MCM helicase single hexamer loaded onto dsDNA.
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8rig
Q-score: 0.44
Miller TCR, Lim CT, Diffley JFX, Costa A
To Be Published
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae S288C)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna (5'-d(p*ap*tp*gp*cp*ap*tp*gp*cp*ap*tp*gp*cp*ap*tp*gp*cp*ap*t)-3') (5 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Hexameric mcm helicase complex loaded onto duplex dna (610 kDa, Complex from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae S288C)
- Zinc ion (65 Da, Ligand)
- Dna (5'-d(p*gp*cp*ap*tp*gp*cp*ap*tp*gp*cp*ap*tp*gp*cp*ap*tp*gp*cp*ap*t)-3') (5 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae S288C)
S. cerevisiae nexus-sCMGE after DNA replication initiation
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8p5e
Q-score: 0.39
Henrikus SS, Gross MH, Willhoft O, Puhringer T, Lewis JS, McClure AW, Greiwe JF, Palm G, Nans A, Diffley JFX, Costa A
Nat Struct Mol Biol (2024) 31 pp. 1265-1276 [ Pubmed: 38760633 DOI: doi:10.1038/s41594-024-01280-z ]
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Dna (19-mer) (5 kDa, DNA from synthetic construct)
- Dna replication complex gins containing psf1, psf2, psf3 and sld5 (Complex from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cell division control protein 45 (75 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna (Complex from synthetic construct)
- Dna replication complex gins protein psf3 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factors mcm2-7 (Complex from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Complex of mcm2-7, cdc45, gins (psf1-3, sld5) dna polymerase epsilon (subunit a and b), 19-mer dna molecule with complementary 7-mer dna molecule (Complex from Saccharomyces cerevisiae)
- Cdc45 (Complex from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna polymerase epsilon contains catalytic subunit a and non-catalytic subunit b (Complex from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Dna (7-mer) (2 kDa, DNA from synthetic construct)
S. cerevisiae ssDNA-sCMGE after DNA replication initiation
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8p62
Q-score: 0.382
Henrikus SS, Gross MH, Willhoft O, Puhringer T, Lewis JS, McClure AW, Greiwe JF, Palm G, Nans A, Diffley JFX, Costa A
Nat Struct Mol Biol (2024) 31 pp. 1265-1276 [ Pubmed: 38760633 DOI: doi:10.1038/s41594-024-01280-z ]
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45, and dna polymerase epsilon subunit a and b (Complex from Saccharomyces cerevisiae)
- Dna replication complex gins containing psf1, psf2, psf3 and sld5 (Complex from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cell division control protein 45 (75 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna (9-mer) (2 kDa, DNA from synthetic construct)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factors mcm2-7 (Complex from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna (9-mer) (Complex from synthetic construct)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Complex of mcm2-7, cdc45, gins (psf1-3, sld5) dna polymerase epsilon (subunit a and b), 9-mer dna molecule (Complex)
S. cerevisiae consensus-sCMGE on ssDNA after DNA replication initiation
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8p63
Q-score: 0.4
Henrikus SS, Gross MH, Willhoft O, Puhringer T, Lewis JS, McClure AW, Greiwe JF, Palm G, Nans A, Diffley JFX, Costa A
Nat Struct Mol Biol (2024) 31 pp. 1265-1276 [ Pubmed: 38760633 DOI: doi:10.1038/s41594-024-01280-z ]
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins containing psf1, psf2, psf3 and sld5 (Complex from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cell division control protein 45 (75 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna (Complex from synthetic construct)
- Dna replication complex gins protein psf3 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna (9-mer) (2 kDa, DNA from synthetic construct)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factors mcm2-7 (Complex from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Cdc45 (Complex from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna polymerase epsilon contains catalytic subunit a and non-catalytic subunit b (Complex from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Complex of mcm2-7, cdc45, gins (psf1-3, sld5) dna polymerase epsilon (subunit a and b), 9-mer dna molecule (Complex)
S. cerevisiae sCMGE with N-ter Mcm10 density
Henrikus SS, Gross MH, Willhoft O, Puhringer T, Lewis JS, McClure AW, Greiwe JF, Palm G, Nans A, Diffley JFX, Costa A
Nat Struct Mol Biol (2024) 31 pp. 1265-1276 [ Pubmed: 38760633 DOI: doi:10.1038/s41594-024-01280-z ]
- Dna replication complex gins containing psf1, psf2, psf3 and sld5 (Complex from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (Protein from Saccharomyces cerevisiae)
- Cell division control protein 45 (Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 10 (Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (Protein from Saccharomyces cerevisiae)
- Dna strand 1 (DNA from Saccharomyces cerevisiae)
- Dna replication licensing factors mcm2-7 (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (Protein from Saccharomyces cerevisiae)
- Complex of mcm2-7, cdc45, gins (psf1-3, sld5) dna polymerase epsilon (subunit a and b), 19-mer dna molecule with complementary 7-mer dna molecule (Complex from Saccharomyces cerevisiae)
- Dna strand 2 (DNA from Saccharomyces cerevisiae)
- Dna (Complex from DNA molecule)
- Cdc45 (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon contains catalytic subunit a and non-catalytic subunit b (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (Protein from DNA molecule)
- Dna replication licensing factor mcm5 (Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf1 (Protein from Saccharomyces cerevisiae)
SsoMCM hexamer bound to Mg/ADP-BeFx and 12-mer oligo-dT. Class 1
Resolution: 2.62 Å
EM Method: Single-particle
Fitted PDBs: 8eaf
Q-score: 0.497
Meagher M, Myasnikov A, Enemark EJ
Int J Mol Sci (2022) 23 [ Pubmed: 36499022 DOI: doi:10.3390/ijms232314678 ]
- 12-mer oligo dt (3 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Ssomcm hexamer bound to mg/adp-befx and 12-mer oligo-dt. class 1. (Complex from Saccharolobus solfataricus P2)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Minichromosome maintenance protein mcm (68 kDa, Protein from Saccharolobus solfataricus P2)
- [[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium (492 Da, Ligand)