Cryo-EM structure of peptide free and Gs-coupled GIPR
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8wa3
Q-score: 0.464
Cong Z, Zhao F, Li Y, Luo G, Mai Y, Chen X, Chen Y, Lin S, Cai X, Zhou Q, Yang D, Wang MW
Cell Discov (2024) 10 pp. 18-18 [ DOI: doi:10.1038/s41421-024-00649-0 Pubmed: 38346960 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1,o-antigen polymerase (40 kDa, Protein from Leptolinea tardivitalis)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Cryo-em structure of the human glucose-dependent insulinotropic polypeptide receptor in complex with gip and g protein (Complex from Homo sapiens)
- Gastric inhibitory polypeptide receptor,fusion protein (64 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
Cryo-EM structure of the gamma-MSH-bound human melanocortin receptor 3 (MC3R)-Gs complex
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8ioc
Q-score: 0.459
Feng W, Zhou Q, Chen X, Dai A, Cai X, Liu X, Zhao F, Chen Y, Ye C, Xu Y, Cong Z, Li H, Lin S, Yang D, Wang MW
Cell Discov (2023) 9 pp. 81- [ Pubmed: 37524700 DOI: doi:10.1107/S0907444909042073 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1,hibit (40 kDa, Protein from Homo sapiens)
- Gamma-melanocyte-stimulating hormone (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Nanobody-35 (17 kDa, Protein from Homo sapiens)
- Cryo-em structure of gamma-msh-bound human melanocortin receptor 3 in complex with g protein (Complex from Homo sapiens)
- Ha signal peptide,melanocortin receptor 3,lgbit subunit (56 kDa, Protein from Homo sapiens)
Cryo-EM structure of the PG-901-bound human melanocortin receptor 5 (MC5R)-Gs complex
Resolution: 2.59 Å
EM Method: Single-particle
Fitted PDBs: 8iod
Q-score: 0.503
Feng W, Zhou Q, Chen X, Dai A, Cai X, Liu X, Zhao F, Chen Y, Ye C, Xu Y, Cong Z, Li H, Lin S, Yang D, Wang MW
Cell Discov (2023) 9 pp. 81- [ Pubmed: 37524700 DOI: doi:10.1107/S0907444909042073 ]
- Cryo-em structure of pg-901-bound human melanocortin receptor 5 in complex with g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1,hibit (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Pg-901 (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Nanobody-35 (17 kDa, Protein from Homo sapiens)
- Ha signal peptide,melanocortin receptor 5,lgbit subunit (56 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human GLP-1R-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7vbh
Q-score: 0.397
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ Pubmed: 35217653 DOI: doi:10.1038/s41467-022-28683-0 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Cryo-em structure of the peptide 20-bound human glucagon-like peptide-1 receptor in complex with gs protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Cryo-em structure of the human glucagon-like peptide-1 receptor in complex with peptide 20 and g protein (Complex from Homo sapiens)
- Peptide 20 (4 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Escherichia coli)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
Cryo-EM structure of the tirzepatide (LY3298176)-bound human GLP-1R-Gs complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7fim
Q-score: 0.409
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ Pubmed: 35217653 DOI: doi:10.1038/s41467-022-28683-0 ]
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Tirzepatide (4 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Cryo-em structure of the human glucagon like peptide 1 receptor in complex with tirzepatide and g protein (Complex from Homo sapiens)
- Glucagon-like peptide 1 receptor,glucagon-like peptide 1 receptor,glucagon-like peptide 1 receptor,glucagon-like peptide 1 receptor,human glucagon like peptide 1 receptor (68 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Rattus norvegicus)
Cryo-EM structure of the tirzepatide-bound human GIPR-Gs complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7fiy
Q-score: 0.413
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ Pubmed: 35217653 DOI: doi:10.1038/s41467-022-28683-0 ]
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Rattus norvegicus)
- Cryo-em structure of the tirzepatide-bound human gipr-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Gastric inhibitory polypeptide receptor,gastric inhibitory polypeptide receptor,human glucose-dependent insulinotropic polypeptide receptor (64 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Tiezepatide (4 kDa, Protein from synthetic construct)
Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human GCGR-Gs complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7v35
Q-score: 0.373
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ Pubmed: 35217653 DOI: doi:10.1038/s41467-022-28683-0 ]
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Cryo-em structure of the gipr/glp-1r/gcgr triagonist peptide 20-bound human gcgr-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Peptide 20 (4 kDa, Protein from synthetic construct)
- Glucagon receptor (46 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
Cryo-EM structure of the non-acylated tirzepatide (LY3298176)-bound human GLP-1R-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7vbi
Q-score: 0.533
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ Pubmed: 35217653 DOI: doi:10.1038/s41467-022-28683-0 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Cryo-em structure of the human glucagon-like peptide-1 receptor in complex with non-acylated_tirzepatide and g protein (Complex from Homo sapiens)
- Isoform 3 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Non-acylated_tirzepatide (3 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Escherichia coli)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human GIPR-Gs complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7fin
Q-score: 0.401
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ Pubmed: 35217653 DOI: doi:10.1038/s41467-022-28683-0 ]
- Cryo-em structure of the human glucose-dependent insulinotropic polypeptide receptor in complex with peptide 20 and g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Gamma-l-glutamic acid (147 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Cholesterol (386 Da, Ligand)
- Peptide 20 (4 kDa, Protein from Homo sapiens)
- Gastric inhibitory polypeptide receptor,human glucose-dependent insulinotropic polypeptide receptor (64 kDa, Protein from Homo sapiens)
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 7dty
Q-score: 0.436
Zhao F, Zhang C, Zhou Q, Hang K, Zou X, Chen Y, Wu F, Rao Q, Dai A, Yin W, Shen DD, Zhang Y, Xia T, Stevens RC, Xu HE, Yang D, Zhao L, Wang MW
eLife (2021) 10 [ DOI: doi:10.7554/eLife.68719 Pubmed: 34254582 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Gastric inhibitory polypeptide (4 kDa, Protein from Homo sapiens)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Human glucose-dependent insulinotropic polypeptide receptor (64 kDa, Protein from Homo sapiens)
- Cryo-em structure of the human glucose-dependent insulinotropic polypeptide receptor in complex with gip and g protein (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)