Cryo-EM structure of a-MSH-MC4R-Gs_Nb35 complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f53
Q-score: 0.474
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Nanobody35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Complex of alpha-msh bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Alpha-melanocyte-stimulating hormones (1 kDa, Protein from synthetic construct)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Calcium ion (40 Da, Ligand)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Alpha-melanocyte-stimulating hormones (Complex)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
Cryo-EM structure of afamelanotide-MC4R-Gs_Nb35 complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f54
Q-score: 0.408
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Afamelanotide (Complex)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Nanobody35 (Complex from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Calcium ion (40 Da, Ligand)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Complex of afamelanotide bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
Cryo-EM structure of bremelanotide-MC4R-Gs_Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f55
Q-score: 0.411
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Bremelanotide (1 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Complex of bremelanotide bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Nanobody35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Bremelanotide (Complex)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
Cryo-EM structure of THIQ-MC4R-Gs_Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f58
Q-score: 0.516
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Nanobody35 (Complex)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Melanocrotin-4 receptor (Complex from Homo sapiens)
- Complex of thiq bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- (3r)-n-[(2r)-3-(4-chlorophenyl)-1-[4-cyclohexyl-4-(1,2,4-triazol-1-ylmethyl)piperidin-1-yl]-1-oxidanylidene-propan-2-yl]-1,2,3,4-tetrahydroisoquinoline-3-carboxamide (589 Da, Ligand)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)