Cryo-EM structure of the Boc5-bound hGLP-1R-Gs complex
Resolution: 2.61 Å
EM Method: Single-particle
Fitted PDBs: 7x8r
Q-score: 0.554
Cong Z, Zhou Q, Li Y, Chen LN, Zhang ZC, Liang A, Liu Q, Wu X, Dai A, Xia T, Wu W, Zhang Y, Yang D, Wang MW
PNAS (2022) 119 pp. e2200155119-e2200155119 [ DOI: doi:10.1073/pnas.2200155119 Pubmed: 35561211 ]
- 2,4-bis(3-methoxy-4-thiophen-2-ylcarbonyloxy-phenyl)-1,3-bis[[4-[(2-methylpropan-2-yl)oxycarbonylamino]phenyl]carbonylamino]cyclobutane-1,3-dicarboxylic acid (1 kDa, Ligand)
- Nanobody 35 (15 kDa, Protein from synthetic construct)
- Cryo-em structure of the human glucagon like peptide 1 receptor in complex with boc5 and g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
Cryo-EM structure of the WB4-24-bound hGLP-1R-Gs complex
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7x8s
Q-score: 0.482
Cong Z, Zhou Q, Li Y, Chen LN, Zhang ZC, Liang A, Liu Q, Wu X, Dai A, Xia T, Wu W, Zhang Y, Yang D, Wang MW
PNAS (2022) 119 pp. e2200155119-e2200155119 [ DOI: doi:10.1073/pnas.2200155119 Pubmed: 35561211 ]
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cryo-em structure of the human glucagon like peptide 1 receptor in complex with wb4-24 and g protein (Complex from Homo sapiens)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
- 2,4-bis(3-methoxy-4-thiophen-2-ylcarbonyloxy-phenyl)-1,3-bis[[4-(2-methylpropanoylamino)phenyl]carbonylamino]cyclobutane-1,3-dicarboxylic acid (1 kDa, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human GLP-1R-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7vbh
Q-score: 0.397
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ Pubmed: 35217653 DOI: doi:10.1038/s41467-022-28683-0 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Cryo-em structure of the peptide 20-bound human glucagon-like peptide-1 receptor in complex with gs protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Cryo-em structure of the human glucagon-like peptide-1 receptor in complex with peptide 20 and g protein (Complex from Homo sapiens)
- Peptide 20 (4 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Escherichia coli)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
Cryo-EM structure of the tirzepatide (LY3298176)-bound human GLP-1R-Gs complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7fim
Q-score: 0.409
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ Pubmed: 35217653 DOI: doi:10.1038/s41467-022-28683-0 ]
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Tirzepatide (4 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Cryo-em structure of the human glucagon like peptide 1 receptor in complex with tirzepatide and g protein (Complex from Homo sapiens)
- Glucagon-like peptide 1 receptor,glucagon-like peptide 1 receptor,glucagon-like peptide 1 receptor,glucagon-like peptide 1 receptor,human glucagon like peptide 1 receptor (68 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Rattus norvegicus)
Cryo-EM structure of the tirzepatide-bound human GIPR-Gs complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7fiy
Q-score: 0.413
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ Pubmed: 35217653 DOI: doi:10.1038/s41467-022-28683-0 ]
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Rattus norvegicus)
- Cryo-em structure of the tirzepatide-bound human gipr-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Gastric inhibitory polypeptide receptor,gastric inhibitory polypeptide receptor,human glucose-dependent insulinotropic polypeptide receptor (64 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Tiezepatide (4 kDa, Protein from synthetic construct)
Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human GCGR-Gs complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7v35
Q-score: 0.373
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ Pubmed: 35217653 DOI: doi:10.1038/s41467-022-28683-0 ]
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Cryo-em structure of the gipr/glp-1r/gcgr triagonist peptide 20-bound human gcgr-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Peptide 20 (4 kDa, Protein from synthetic construct)
- Glucagon receptor (46 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
Cryo-EM structure of the non-acylated tirzepatide (LY3298176)-bound human GLP-1R-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7vbi
Q-score: 0.533
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ Pubmed: 35217653 DOI: doi:10.1038/s41467-022-28683-0 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Cryo-em structure of the human glucagon-like peptide-1 receptor in complex with non-acylated_tirzepatide and g protein (Complex from Homo sapiens)
- Isoform 3 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Non-acylated_tirzepatide (3 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Escherichia coli)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human GIPR-Gs complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7fin
Q-score: 0.401
Zhao F, Zhou Q, Cong Z, Hang K, Zou X, Zhang C, Chen Y, Dai A, Liang A, Ming Q, Wang M, Chen LN, Xu P, Chang R, Feng W, Xia T, Zhang Y, Wu B, Yang D, Zhao L, Xu HE, Wang MW
Nat Commun (2022) 13 pp. 1057-1057 [ Pubmed: 35217653 DOI: doi:10.1038/s41467-022-28683-0 ]
- Cryo-em structure of the human glucose-dependent insulinotropic polypeptide receptor in complex with peptide 20 and g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Gamma-l-glutamic acid (147 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Cholesterol (386 Da, Ligand)
- Peptide 20 (4 kDa, Protein from Homo sapiens)
- Gastric inhibitory polypeptide receptor,human glucose-dependent insulinotropic polypeptide receptor (64 kDa, Protein from Homo sapiens)
Cryo-EM structure of a-MSH-MC4R-Gs_Nb35 complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f53
Q-score: 0.474
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Nanobody35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Complex of alpha-msh bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Alpha-melanocyte-stimulating hormones (1 kDa, Protein from synthetic construct)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Calcium ion (40 Da, Ligand)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Alpha-melanocyte-stimulating hormones (Complex)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
Cryo-EM structure of afamelanotide-MC4R-Gs_Nb35 complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f54
Q-score: 0.408
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Afamelanotide (Complex)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Nanobody35 (Complex from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Calcium ion (40 Da, Ligand)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Complex of afamelanotide bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)