Cryo-EM structure of a-MSH-MC4R-Gs_Nb35 complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f53
Q-score: 0.474
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ DOI: doi:10.1038/s41422-021-00552-3 Pubmed: 34433901 ]
- Calcium ion (40 Da, Ligand)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Alpha-melanocyte-stimulating hormones (Complex)
- Melanocrotin-4 receptor (Complex)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Alpha-melanocyte-stimulating hormones (1 kDa, Protein from synthetic construct)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Complex of alpha-msh bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Nanobody35 (Complex from Homo sapiens)
Cryo-EM structure of afamelanotide-MC4R-Gs_Nb35 complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f54
Q-score: 0.408
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ DOI: doi:10.1038/s41422-021-00552-3 Pubmed: 34433901 ]
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Afamelanotide (Complex)
- Calcium ion (40 Da, Ligand)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Complex of afamelanotide bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Nanobody35 (Complex from Homo sapiens)
Cryo-EM structure of bremelanotide-MC4R-Gs_Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f55
Q-score: 0.411
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ DOI: doi:10.1038/s41422-021-00552-3 Pubmed: 34433901 ]
- Water (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Complex of bremelanotide bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Bremelanotide (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Bremelanotide (Complex)
- Nanobody35 (Complex from Homo sapiens)
Cryo-EM structure of THIQ-MC4R-Gs_Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f58
Q-score: 0.516
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ DOI: doi:10.1038/s41422-021-00552-3 Pubmed: 34433901 ]
- Calcium ion (40 Da, Ligand)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- (3r)-n-[(2r)-3-(4-chlorophenyl)-1-[4-cyclohexyl-4-(1,2,4-triazol-1-ylmethyl)piperidin-1-yl]-1-oxidanylidene-propan-2-yl]-1,2,3,4-tetrahydroisoquinoline-3-carboxamide (589 Da, Ligand)
- Nanobody35 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Melanocrotin-4 receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Complex of thiq bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
Cryo-EM structure of the human growth hormone-releasing hormone receptor-Gs protein complex
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7cz5
Q-score: 0.582
Zhou F, Zhang H, Cong Z, Zhao LH, Zhou Q, Mao C, Cheng X, Shen DD, Cai X, Ma C, Wang Y, Dai A, Zhou Y, Sun W, Zhao F, Zhao S, Jiang H, Jiang Y, Yang D, Eric Xu H, Zhang Y, Wang MW
Nat Commun (2020) 11 pp. 5205-5205 [ Pubmed: 33060564 DOI: doi:10.1038/s41467-020-18945-0 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (43 kDa, Protein from Rattus norvegicus)
- Nanobody35 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Somatoliberin (5 kDa, Protein from Homo sapiens)
- Hormone (Complex from Homo sapiens)
- Growth hormone-releasing hormone receptor,growth hormone-releasing hormone receptor (61 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- G(s) subunit beta-1 (Complex from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Growth hormone-releasing hormone receptor - gs protein complex (180 kDa, Complex)
- G(s) subunit gamma-2 (Complex from Bos taurus)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Hormone receptor, g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)