Cryo-EM structure of the PAC1R-PACAP27-Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8e3x
Q-score: 0.543
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ DOI: doi:10.1038/s41467-022-34629-3 Pubmed: 36385145 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, pituitary adenylate cyclase-activating polypeptide type i receptor (Complex from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Pituitary adenylate cyclase-activating polypeptide type i receptor (55 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase activating polypeptide-27 (Complex from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide (3 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Gs coupled pituitary adenylate cyclase-activating polypeptide type i receptor complex with pacap27 peptide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Cryo-EM structure of the VPAC1R-PACAP27-Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8e3y
Q-score: 0.576
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ DOI: doi:10.1038/s41467-022-34629-3 Pubmed: 36385145 ]
- Vasoactive intestinal polypeptide receptor 1 (52 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Pituitary adenylate cyclase activating polypeptide-27 (Complex from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, vasoactive intestinal polypeptide receptor 1 (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Gs coupled vasoactive intestinal polypeptide receptor 1 (vpac1) complex with pacap27 peptide (Complex)
Cryo-EM structure of the VPAC1R-VIP-Gs complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8e3z
Q-score: 0.483
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ Pubmed: 36385145 DOI: doi:10.1038/s41467-022-34629-3 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, vasoactive intestinal polypeptide receptor 1 (Complex from Homo sapiens)
- Vasoactive intestinal peptide-vip (Complex from Homo sapiens)
- Vasoactive intestinal peptide (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Nanobody35 (Complex from Lama glama)
- Vasoactive intestinal polypeptide receptor 1 (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Gs coupled vasoactive intestinal polypeptide receptor 1 (vpac1) complex with vip peptide (Complex)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Human secretin receptor Gs complex
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6wi9
Q-score: 0.359
Dong M, Deganutti G, Piper SJ, Liang YL, Khoshouei M, Belousoff MJ, Harikumar KG, Reynolds CA, Glukhova A, Furness SGB, Christopoulos A, Danev R, Wootten D, Sexton PM, Miller LJ
Nat Commun (2020) 11 pp. 4137-4137 [ Pubmed: 32811827 DOI: doi:10.1038/s41467-020-17791-4 ]
- Nanobody35 (15 kDa, Protein from Lama glama)
- Secretin receptor (51 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, secretin receptor (Complex from Homo sapiens)
- Secretin (3 kDa, Protein from Homo sapiens)
- Secretin (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gs coupled sectretin complex with secretin peptide (Complex)
Human secretin receptor Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 6wzg
Q-score: 0.609
Dong M, Deganutti G, Piper SJ, Liang YL, Khoshouei M, Belousoff MJ, Harikumar KG, Reynolds CA, Glukhova A, Furness SGB, Christopoulos A, Danev R, Wootten D, Sexton PM, Miller LJ
Nat Commun (2020) 11 pp. 4137-4137 [ Pubmed: 32811827 DOI: doi:10.1038/s41467-020-17791-4 ]
- Secretin receptor (51 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gs coupled secretin complex with secretin peptide (Complex)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, secretin receptor (Complex from Homo sapiens)
- Secretin (Complex from Homo sapiens)
- Secretin (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 6uus
Q-score: 0.535
Liang YL, Belousoff MJ, Fletcher MM, Zhang X, Khoshouei M, Deganutti G, Koole C, Furness SGB, Miller LJ, Hay DL, Christopoulos A, Reynolds CA, Danev R, Wootten D, Sexton PM
Acs Pharmacol Transl Sci (2020) 3 pp. 263-284 [ Pubmed: 32296767 DOI: doi:10.1021/acsptsci.9b00080 ]
- Calcitonin gene-related peptide type 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Adm (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, receptor activity-modifying protein 3, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, calcitonin gene-related peptide type 1 receptor (Complex from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Adm (6 kDa, Protein from Homo sapiens)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Am2:clr:ramp3:gasdn:gb1:gg2:nb35 gpcr complex (Complex)
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 6uva
Q-score: 0.553
Liang YL, Belousoff MJ, Fletcher MM, Zhang X, Khoshouei M, Deganutti G, Koole C, Furness SGB, Miller LJ, Hay DL, Christopoulos A, Reynolds CA, Danev R, Wootten D, Sexton PM
Acs Pharmacol Transl Sci (2020) 3 pp. 263-284 [ Pubmed: 32296767 DOI: doi:10.1021/acsptsci.9b00080 ]
- Protein adm2 (5 kDa, Protein from Homo sapiens)
- Calcitonin gene-related peptide type 1 receptor (56 kDa, Protein from Homo sapiens)
- Am2:clr:ramp3:gasdn:gb1:gg2:nb35 gpcr complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, receptor activity-modifying protein 3, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, calcitonin gene-related peptide type 1 receptor (Complex from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Protein adm2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6uun
Q-score: 0.48
Liang YL, Belousoff MJ, Fletcher MM, Zhang X, Khoshouei M, Deganutti G, Koole C, Furness SGB, Miller LJ, Hay DL, Christopoulos A, Reynolds CA, Danev R, Wootten D, Sexton PM
Acs Pharmacol Transl Sci (2020) 3 pp. 263-284 [ Pubmed: 32296767 DOI: doi:10.1021/acsptsci.9b00080 ]
- Calcitonin gene-related peptide type 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, receptor activity-modifying protein 2, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, calcitonin gene-related peptide type 1 receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Adm (Complex from Homo sapiens)
- Am:clr:ramp2:gasdn:gb1:gg2:nb35 gpcr complex (Complex)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Adm (6 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Receptor activity-modifying protein 2 (17 kDa, Protein from Homo sapiens)
Non-peptide agonist (TT-OAD2) bound to the Glucagon-Like peptide-1 (GLP-1) Receptor
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6orv
Q-score: 0.538
Zhao P, Liang YL, Belousoff MJ, Deganutti G, Fletcher MM, Willard FS, Bell MG, Christe ME, Sloop KW, Inoue A, Truong TT, Clydesdale L, Furness SGB, Christopoulos A, Wang MW, Miller LJ, Reynolds CA, Danev R, Sexton PM, Wootten D
Nature (2020) 577 pp. 432-436 [ DOI: doi:10.1038/s41586-019-1902-z Pubmed: 31915381 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, glucagon-like peptide 1 receptor (Complex from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Homo sapiens)
- Tt-oad2:glp-1r:gs complex (120 kDa, Complex)
- N-{(3s,8s)-3-{4-[(3,4-dichlorophenyl)methoxy]phenyl}-7-[(1s)-1-phenylpropyl]-2,3,6,7,8,9-hexahydro[1,4]dioxino[2,3-g]isoquinoline-8-carbonyl}-4-(2,3-dimethylpyridin-4-yl)-l-phenylalanine (856 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
Cryo-EM structure of the active, Gs-protein complexed, human CGRP receptor
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6e3y
Q-score: 0.429
Liang YL, Khoshouei M, Deganutti G, Glukhova A, Koole C, Peat TS, Radjainia M, Plitzko JM, Baumeister W, Miller LJ, Hay DL, Christopoulos A, Reynolds CA, Wootten D, Sexton PM
Nature (2018) 561 pp. 492-497 [ DOI: doi:10.1038/s41586-018-0535-y Pubmed: 30209400 ]
- Receptor activity-modifying protein 1 (17 kDa, Protein from Homo sapiens)
- Calcitonin gene-related peptide type 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Calcitonin gene-related peptide 1 (3 kDa, Protein from Homo sapiens)
- Complex of a full-length, active-state calcitonin gene-related peptide receptor with calcitonin gene-related peptide ligand, heterotrimeric gs protein and nanobody35 (Complex from Homo sapiens)