Cryo-EM structure of the PAC1R-PACAP27-Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8e3x
Q-score: 0.543
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ DOI: doi:10.1038/s41467-022-34629-3 Pubmed: 36385145 ]
- Nanobody35 (15 kDa, Protein from Lama glama)
- Pituitary adenylate cyclase activating polypeptide-27 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide type i receptor (55 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, pituitary adenylate cyclase-activating polypeptide type i receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide (3 kDa, Protein from Homo sapiens)
- Gs coupled pituitary adenylate cyclase-activating polypeptide type i receptor complex with pacap27 peptide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
Cryo-EM structure of the VPAC1R-PACAP27-Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8e3y
Q-score: 0.576
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ DOI: doi:10.1038/s41467-022-34629-3 Pubmed: 36385145 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Pituitary adenylate cyclase activating polypeptide-27 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Vasoactive intestinal polypeptide receptor 1 (52 kDa, Protein from Homo sapiens)
- Gs coupled vasoactive intestinal polypeptide receptor 1 (vpac1) complex with pacap27 peptide (Complex)
- Nanobody35 (Complex from Lama glama)
- Pituitary adenylate cyclase-activating polypeptide (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, vasoactive intestinal polypeptide receptor 1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 6uus
Q-score: 0.535
Liang YL, Belousoff MJ, Fletcher MM, Zhang X, Khoshouei M, Deganutti G, Koole C, Furness SGB, Miller LJ, Hay DL, Christopoulos A, Reynolds CA, Danev R, Wootten D, Sexton PM
Acs Pharmacol Transl Sci (2020) 3 pp. 263-284 [ Pubmed: 32296767 DOI: doi:10.1021/acsptsci.9b00080 ]
- Am2:clr:ramp3:gasdn:gb1:gg2:nb35 gpcr complex (Complex)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Adm (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, receptor activity-modifying protein 3, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, calcitonin gene-related peptide type 1 receptor (Complex from Homo sapiens)
- Calcitonin gene-related peptide type 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Adm (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 6uva
Q-score: 0.553
Liang YL, Belousoff MJ, Fletcher MM, Zhang X, Khoshouei M, Deganutti G, Koole C, Furness SGB, Miller LJ, Hay DL, Christopoulos A, Reynolds CA, Danev R, Wootten D, Sexton PM
Acs Pharmacol Transl Sci (2020) 3 pp. 263-284 [ Pubmed: 32296767 DOI: doi:10.1021/acsptsci.9b00080 ]
- Am2:clr:ramp3:gasdn:gb1:gg2:nb35 gpcr complex (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, receptor activity-modifying protein 3, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, calcitonin gene-related peptide type 1 receptor (Complex from Homo sapiens)
- Calcitonin gene-related peptide type 1 receptor (56 kDa, Protein from Homo sapiens)
- Protein adm2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Protein adm2 (5 kDa, Protein from Homo sapiens)