Structure of human class T GPCR TAS2R14-miniGs/gust complex with Aristolochic acid A.
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 8xql
Q-score: 0.469
Hu X, Ao W, Gao M, Wu L, Pei Y, Liu S, Wu Y, Zhao F, Sun Q, Liu J, Jiang L, Wang X, Li Y, Tan Q, Cheng J, Yang F, Yang C, Sun J, Hua T, Liu ZJ
Nature (2024) 631 pp. 459-466 [ Pubmed: 38776963 DOI: doi:10.1038/s41586-024-07569-9 ]
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Exo-alpha-sialidase,taste receptor type 2 member 14,lgbit (110 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (14 kDa, Protein from Homo sapiens)
- 8-methoxy-6-nitro-naphtho[1,2-e][1,3]benzodioxole-5-carboxylic acid (341 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Complex of tas2r14-minigsg with aristolochic acid a (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Structure 2 of human class T GPCR TAS2R14-miniGs/gust complex with Flufenamic acid.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8xqr
Q-score: 0.386
Hu X, Ao W, Gao M, Wu L, Pei Y, Liu S, Wu Y, Zhao F, Sun Q, Liu J, Jiang L, Wang X, Li Y, Tan Q, Cheng J, Yang F, Yang C, Sun J, Hua T, Liu ZJ
Nature (2024) 631 pp. 459-466 [ Pubmed: 38776963 DOI: doi:10.1038/s41586-024-07569-9 ]
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Exo-alpha-sialidase,taste receptor type 2 member 14,lgbit (110 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Structure 2 of human class t gpcr tas2r14-minigs/gust complex with flufenamic acid. (Complex from Homo sapiens)
- Nanobody 35 (14 kDa, Protein from Homo sapiens)
- 2-[[3-(trifluoromethyl)phenyl]amino] benzoic acid (281 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class T GPCR in apo state
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 7xp4
Q-score: 0.428
Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ, Hua T
Science (2022) 377 pp. 1298-1304 [ Pubmed: 36108005 DOI: doi:10.1126/science.abo1633 ]
- Endoglucanase h,taste receptor type 2 member 46,endoglucanase h,taste receptor type 2 member 46,bitter taste receptor t2r46 (91 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Complex of gpcr and g protein (Complex from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class T GPCR in ligand-free state
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7xp5
Q-score: 0.412
Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ, Hua T
Science (2022) 377 pp. 1298-1304 [ DOI: doi:10.1126/science.abo1633 Pubmed: 36108005 ]
- Endoglucanase h,taste receptor type 2 member 46,bitter taste receptor t2r46 (91 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Complex of gpcr and g protein (Complex from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class T GPCR in active state
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 7xp6
Q-score: 0.415
Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ, Hua T
Science (2022) 377 pp. 1298-1304 [ DOI: doi:10.1126/science.abo1633 Pubmed: 36108005 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Endoglucanase h,taste receptor type 2 member 46,endoglucanase h,taste receptor type 2 member 46,bitter taste receptor t2r46 (91 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Complex of gpcr and g protein (Complex from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
- Strychnine (334 Da, Ligand)