Human proton sensing receptor GPR65 in complex with miniGs - focused map of 7TM
Howard MK, Hoppe N, Huang XP, Mitrovic D, Billesbolle CB, Macdonald CB, Mehrotra E, Rockefeller Grimes P, Trinidad DD, Delemotte L, English JG, Coyote-Maestas W, Manglik A
Cell (2024) [ DOI: doi:10.1016/j.cell.2024.11.036 Pubmed: 39753132 ]
- Gpr65 receptor in complex with minigs (Complex)
- Nanobody 35 (Complex from Lama glama)
- Minigs (alpha and beta subunits) (Complex from Homo sapiens)
- Gpr65 receptor (Complex from Homo sapiens)
Human proton sensing receptor GPR65 in complex with miniGs
Howard MK, Hoppe N, Huang XP, Mitrovic D, Billesbolle CB, Macdonald CB, Mehrotra E, Rockefeller Grimes P, Trinidad DD, Delemotte L, English JG, Coyote-Maestas W, Manglik A
Cell (2025) 188 pp. 671- [ Pubmed: 39753132 DOI: doi:10.1016/j.cell.2024.11.036 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Psychosine receptor (40 kDa, Protein from Homo sapiens)
- Complex of gpr65 bound to gs heterotrimer (Complex)
Human proton sensing receptor GPR68 in complex with miniGs
Howard MK, Hoppe N, Huang XP, Mitrovic D, Billesbolle CB, Macdonald CB, Mehrotra E, Rockefeller Grimes P, Trinidad DD, Delemotte L, English JG, Coyote-Maestas W, Manglik A
Cell (2025) 188 pp. 671- [ Pubmed: 39753132 DOI: doi:10.1016/j.cell.2024.11.036 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Complex of gpr68 bound to gs heterotrimer (Complex)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Ovarian cancer g-protein coupled receptor 1 (42 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GLP-1(9-36)-bound human GLP-1R-Gs complex in the presence of LSN3318839
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 9ivm
Q-score: 0.455
Li J, Li G, Mai Y, Liu X, Yang D, Zhou Q, Wang MW
Acta Pharm Sin B (2024) 14 pp. 5069-5073 [ Pubmed: 39664415 DOI: doi:10.1016/j.apsb.2024.09.002 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cryo-em structure of the glp-1(9-36)-bound human glp-1r-gs complex in the presence of lsn3318839 (Complex from Homo sapiens)
- (2~{r})-2-[2-[1-[(1~{r})-1-[2,6-bis(chloranyl)-3-cyclopropyl-phenyl]ethyl]imidazo[4,5-c]pyridin-6-yl]phenyl]propanoic acid (480 Da, Ligand)
- Nanobody-35 (15 kDa, Protein from Homo sapiens)
- Glp-1(9-36) (3 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
Consensus olfactory receptor consOR51 in complex with mini-Gs trimeric protein
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8uxv
Q-score: 0.544
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08126-0 Pubmed: 39478229 ]
- Consor51 (36 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Minigs399 (30 kDa, Protein from Homo sapiens)
- Consor51-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
Consensus olfactory receptor consOR1 bound to L-menthol and in complex with mini-Gs trimeric protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8uxy
Q-score: 0.402
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08126-0 Pubmed: 39478229 ]
- (1r,2s,5r)-5-methyl-2-(propan-2-yl)cyclohexan-1-ol (156 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Consor1 (35 kDa, Protein from synthetic construct)
- Gnas complex locus (30 kDa, Protein from Homo sapiens)
- Consor51-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
Consensus olfactory receptor consOR2 bound to S-carvone and in complex with mini-Gs trimeric protein
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8uy0
Q-score: 0.489
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08126-0 Pubmed: 39478229 ]
- Consor2 (35 kDa, Protein from synthetic construct)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- (5s)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one (150 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Consor2-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
- Minigs399 (30 kDa, Protein from Homo sapiens)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8uyq
Q-score: 0.262
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ Pubmed: 39478229 DOI: doi:10.1038/s41586-024-08126-0 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Gnas complex locus (30 kDa, Protein from Homo sapiens)
- Consor4 (36 kDa, Protein from synthetic construct)
- (2r)-2-methyl-1,3-thiazolidine (103 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Consor4-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
Cryo-EM structure of the retatrutide-bound human GLP-1R-Gs complex
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 8yw3
Q-score: 0.514
Li W, Zhou Q, Cong Z, Yuan Q, Li W, Zhao F, Xu HE, Zhao LH, Yang D, Wang MW
Cell Discov (2024) 10 pp. 77-77 [ Pubmed: 39019866 DOI: doi:10.1038/s41421-024-00700-0 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Cryo-em structure of the human glucagon-like peptide-1 receptor in complex with retatrutide and g protein (Complex from Homo sapiens)
- Retatrutide (3 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (50 kDa, Protein from Homo sapiens)
Cryo-EM structure of the retatrutide-bound human GIPR-Gs complex
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 8yw4
Q-score: 0.379
Li W, Zhou Q, Cong Z, Yuan Q, Li W, Zhao F, Xu HE, Zhao LH, Yang D, Wang MW
Cell Discov (2024) 10 pp. 77-77 [ Pubmed: 39019866 DOI: doi:10.1038/s41421-024-00700-0 ]
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Gastric inhibitory polypeptide receptor (65 kDa, Protein from Homo sapiens)
- Mini-gs (41 kDa, Protein from Homo sapiens)
- Cryo-em structure of the retatrutide-bound human gipr-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Retatrutide (3 kDa, Protein from Homo sapiens)