Cryo-EM structure of the PAC1R-PACAP27-Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8e3x
Q-score: 0.543
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ DOI: doi:10.1038/s41467-022-34629-3 Pubmed: 36385145 ]
- Nanobody35 (15 kDa, Protein from Lama glama)
- Pituitary adenylate cyclase activating polypeptide-27 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide type i receptor (55 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, pituitary adenylate cyclase-activating polypeptide type i receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide (3 kDa, Protein from Homo sapiens)
- Gs coupled pituitary adenylate cyclase-activating polypeptide type i receptor complex with pacap27 peptide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
Cryo-EM structure of the VPAC1R-PACAP27-Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8e3y
Q-score: 0.576
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ DOI: doi:10.1038/s41467-022-34629-3 Pubmed: 36385145 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Pituitary adenylate cyclase activating polypeptide-27 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Vasoactive intestinal polypeptide receptor 1 (52 kDa, Protein from Homo sapiens)
- Gs coupled vasoactive intestinal polypeptide receptor 1 (vpac1) complex with pacap27 peptide (Complex)
- Nanobody35 (Complex from Lama glama)
- Pituitary adenylate cyclase-activating polypeptide (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, vasoactive intestinal polypeptide receptor 1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
Cryo-EM structure of the VPAC1R-VIP-Gs complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8e3z
Q-score: 0.483
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ DOI: doi:10.1038/s41467-022-34629-3 Pubmed: 36385145 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Gs coupled vasoactive intestinal polypeptide receptor 1 (vpac1) complex with vip peptide (Complex)
- Water (18 Da, Ligand)
- Vasoactive intestinal polypeptide receptor 1 (52 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Vasoactive intestinal peptide-vip (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Vasoactive intestinal peptide (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, vasoactive intestinal polypeptide receptor 1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
Human Amylin2 Receptor in complex with Gs and salmon calcitonin peptide
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7tyy
Q-score: 0.509
Cao J, Belousoff MJ, Liang YL, Johnson RM, Josephs TM, Fletcher MM, Christopoulos A, Hay DL, Danev R, Wootten D, Sexton PM
Science (2022) 375 pp. eabm9609-eabm9609 [ Pubmed: 35324283 DOI: doi:10.1126/science.abm9609 ]
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Human amylin 2 receptor in complex with gs and salmon calcitonin peptide (Complex)
- Water (18 Da, Ligand)
- Calcitonin-1 (3 kDa, Protein from Salmo salar)
- Receptor activity-modifying protein 2 (17 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 6x18
Q-score: 0.563
Zhang X, Belousoff MJ, Zhao P, Kooistra AJ, Truong TT, Ang SY, Underwood CR, Egebjerg T, Senel P, Stewart GD, Liang YL, Glukhova A, Venugopal H, Christopoulos A, Furness SGB, Miller LJ, Reedtz-Runge S, Langmead CJ, Gloriam DE, Danev R, Sexton PM, Wootten D
Mol Cell (2020) 80 pp. 485- [ Pubmed: 33027691 DOI: doi:10.1016/j.molcel.2020.09.020 ]
- Glp-1 peptide hormone bound to glucagon-like peptide-1 (glp-1) receptor (120 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Glucagon (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Water (18 Da, Ligand)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, glucagon-like peptide 1 receptor (Complex from Homo sapiens)
Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 6x19
Q-score: 0.579
Zhang X, Belousoff MJ, Zhao P, Kooistra AJ, Truong TT, Ang SY, Underwood CR, Egebjerg T, Senel P, Stewart GD, Liang YL, Glukhova A, Venugopal H, Christopoulos A, Furness SGB, Miller LJ, Reedtz-Runge S, Langmead CJ, Gloriam DE, Danev R, Sexton PM, Wootten D
Mol Cell (2020) 80 pp. 485- [ Pubmed: 33027691 DOI: doi:10.1016/j.molcel.2020.09.020 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- 3-{(1s)-1-[5-(2,2-dimethylmorpholin-4-yl)-2-{(4s)-2-(4-fluoro-3,5-dimethylphenyl)-3-[3-(4-fluoro-1-methyl-1h-indazol-5-yl)-2-oxo-2,3-dihydro-1h-imidazol-1-yl]-4-methyl-2,4,6,7-tetrahydro-5h-pyrazolo[4,3-c]pyridine-5-carbonyl}-1h-indol-1-yl]butyl}-1,2,4-oxadiazol-5(4h)-one (885 Da, Ligand)
- Chu-128:glp-1r:gs complex (120 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, glucagon-like peptide 1 receptor (Complex from Homo sapiens)
Non peptide agonist PF-06882961, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 6x1a
Q-score: 0.522
Zhang X, Belousoff MJ, Zhao P, Kooistra AJ, Truong TT, Ang SY, Underwood CR, Egebjerg T, Senel P, Stewart GD, Liang YL, Glukhova A, Venugopal H, Christopoulos A, Furness SGB, Miller LJ, Reedtz-Runge S, Langmead CJ, Gloriam DE, Danev R, Sexton PM, Wootten D
Mol Cell (2020) 80 pp. 485- [ Pubmed: 33027691 DOI: doi:10.1016/j.molcel.2020.09.020 ]
- Pf-06882961:glp-1r:gs complex (120 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- 2-[(4-{6-[(4-cyano-2-fluorophenyl)methoxy]pyridin-2-yl}piperidin-1-yl)methyl]-1-{[(2s)-oxetan-2-yl]methyl}-1h-benzimidazole-6-carboxylic acid (555 Da, Ligand)
- Nanobody35 (Complex from Lama glama)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, glucagon-like peptide 1 receptor (Complex from Homo sapiens)
Human secretin receptor Gs complex
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6wi9
Q-score: 0.359
Dong M, Deganutti G, Piper SJ, Liang YL, Khoshouei M, Belousoff MJ, Harikumar KG, Reynolds CA, Glukhova A, Furness SGB, Christopoulos A, Danev R, Wootten D, Sexton PM, Miller LJ
Nat Commun (2020) 11 pp. 4137-4137 [ Pubmed: 32811827 DOI: doi:10.1038/s41467-020-17791-4 ]
- Secretin (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Secretin (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Gs coupled sectretin complex with secretin peptide (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, secretin receptor (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Secretin receptor (51 kDa, Protein from Homo sapiens)
Human secretin receptor Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 6wzg
Q-score: 0.609
Dong M, Deganutti G, Piper SJ, Liang YL, Khoshouei M, Belousoff MJ, Harikumar KG, Reynolds CA, Glukhova A, Furness SGB, Christopoulos A, Danev R, Wootten D, Sexton PM, Miller LJ
Nat Commun (2020) 11 pp. 4137-4137 [ Pubmed: 32811827 DOI: doi:10.1038/s41467-020-17791-4 ]
- Secretin (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Secretin (3 kDa, Protein from Homo sapiens)
- Gs coupled secretin complex with secretin peptide (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, secretin receptor (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Secretin receptor (51 kDa, Protein from Homo sapiens)
CryoEM Structure of the glucagon receptor with a dual-agonist peptide
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6whc
Q-score: 0.445
Chang R, Zhang X, Qiao A, Dai A, Belousoff MJ, Tan Q, Shao L, Zhong L, Lin G, Liang YL, Ma L, Han S, Yang D, Danev R, Wang MW, Wootten D, Wu B, Sexton PM
J Biol Chem (2020) 295 pp. 9313-9325 [ Pubmed: 32371397 DOI: doi:10.1074/jbc.RA120.013793 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Glucagon receptor complex (Complex)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Dual-agonist peptide (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, receptor activity-modifying protein 3, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, calcitonin gene-related peptide type 1 receptor, glucagon receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Dual-agonist peptide (3 kDa, Protein from synthetic construct)
- Glucagon receptor (54 kDa, Protein from Homo sapiens)