Cryo-EM structure of the DMCHA-bound mTAAR9-Gs complex
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 8itf
Q-score: 0.491
Guo L, Cheng J, Lian S, Liu Q, Lu Y, Zheng Y, Zhu K, Zhang M, Kong Y, Zhang C, Rong N, Zhuang Y, Fang G, Jiang J, Zhang T, Han X, Liu Z, Xia M, Liu S, Zhang L, Liberles SD, Yu X, Xu Y, Yang F, Li Q, Sun JP
Nature (2023) 618 pp. 193-200 [ Pubmed: 37225986 DOI: doi:10.1038/s41586-023-06106-4 ]
- Trace amine-associated receptor 9 (38 kDa, Protein from Mus musculus)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (42 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- ~{n},~{n}-dimethylcyclohexanamine (127 Da, Ligand)
- Cryo-em structure of the dmcha-bound mtaar9-gs complex (Complex from Mus musculus)
Cryo-EM structure of the CAD-bound mTAAR9-Gs complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8iw9
Q-score: 0.546
Guo L, Cheng J, Lian S, Liu Q, Lu Y, Zheng Y, Zhu K, Zhang M, Kong Y, Zhang C, Rong N, Zhuang Y, Fang G, Jiang J, Zhang T, Han X, Liu Z, Xia M, Liu S, Zhang L, Liberles SD, Yu X, Xu Y, Yang F, Li Q, Sun JP
Nature (2023) 618 pp. 193-200 [ Pubmed: 37225986 DOI: doi:10.1038/s41586-023-06106-4 ]
- Cryo-em structure of the cad-bound mtaar9-gs complex (Complex from Mus musculus)
- Trace amine-associated receptor 9 (38 kDa, Protein from Mus musculus)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (42 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
Cryo-EM structure of the fenoldopam-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7x2c
Q-score: 0.465
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ DOI: doi:10.1038/s41467-022-30929-w Pubmed: 35676276 ]
- Nanobody35 (Complex)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Nanobody35 (17 kDa, Protein from synthetic construct)
- Cryo-em structure of the fenoldopam-bound d1r-minigs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- (1r)-6-chloranyl-1-(4-hydroxyphenyl)-2,3,4,5-tetrahydro-1h-3-benzazepine-7,8-diol (305 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Gbeta-1gama-2 (Complex from Homo sapiens)
- The fenoldopam-bound d1r-minigs (Complex)
Cryo-EM structure of the tavapadon-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7x2d
Q-score: 0.495
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ Pubmed: 35676276 DOI: doi:10.1038/s41467-022-30929-w ]
- Tavapadon-bound d1r-minigs (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nanobody35 (17 kDa, Protein from synthetic construct)
- Cryo-em structure of the tavapadon-bound d1r-minigs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- 1,5-dimethyl-6-[2-methyl-4-[3-(trifluoromethyl)pyridin-2-yl]oxy-phenyl]pyrimidine-2,4-dione (391 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex)
Cryo-EM structure of the dopamine and LY3154207-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7x2f
Q-score: 0.471
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ Pubmed: 35676276 DOI: doi:10.1038/s41467-022-30929-w ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nanobody35 (17 kDa, Protein from synthetic construct)
- 2-[2,6-bis(chloranyl)phenyl]-1-[(1s,3r)-3-(hydroxymethyl)-1-methyl-5-(3-methyl-3-oxidanyl-butyl)-3,4-dihydro-1h-isoquinolin-2-yl]ethanone (450 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cryo-em structure of the dopamine-ly3154207-bound d1r-minigs complexthe (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Dopamine-ly3154207-bound d1r-minigs (Complex)
- Nanobody35 (Complex)
Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7wui
Q-score: 0.418
Xiao P, Guo S, Wen X, He QT, Lin H, Huang SM, Gou L, Zhang C, Yang Z, Zhong YN, Yang CC, Li Y, Gong Z, Tao XN, Yang ZS, Lu Y, Li SL, He JY, Wang C, Zhang L, Kong L, Sun JP, Yu X
Nature (2022) 604 pp. 771-778 [ DOI: doi:10.1038/s41586-022-04590-8 Pubmed: 35418677 ]
- Cryo-em structure of the adgrg2-fl-ip15-gs complex (253 MDa, Complex)
- Nanobody-35 (13 kDa, Protein from Lama glama)
- Gs (Complex from Homo sapiens)
- Ip15 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Adhesion g-protein coupled receptor g2,mcherry (126 kDa, Protein from Mus musculus)
- Ip15 (1 kDa, Protein from synthetic construct)
- Nanobody-35 (Complex from Lama glama)
- Adhesion g-protein coupled receptor g2 (Complex from Mus musculus)
- Mini-gs (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from synthetic construct)
Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7wuj
Q-score: 0.474
Xiao P, Guo S, Wen X, He QT, Lin H, Huang SM, Gou L, Zhang C, Yang Z, Zhong YN, Yang CC, Li Y, Gong Z, Tao XN, Yang ZS, Lu Y, Li SL, He JY, Wang C, Zhang L, Kong L, Sun JP, Yu X
Nature (2022) 604 pp. 771-778 [ DOI: doi:10.1038/s41586-022-04590-8 Pubmed: 35418677 ]
- Nanobody-35 (13 kDa, Protein from Lama glama)
- Gs (Complex from Homo sapiens)
- Adhesion g-protein coupled receptor g4,uncharacterized protein (71 kDa, Protein from Psychromonas sp. B3M02)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody-35 (Complex from Lama glama)
- Mini-gs (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from synthetic construct)
- Cryo-em structure of the adgr4-beta-gs complex (Complex)
Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7wuq
Q-score: 0.465
Xiao P, Guo S, Wen X, He QT, Lin H, Huang SM, Gou L, Zhang C, Yang Z, Zhong YN, Yang CC, Li Y, Gong Z, Tao XN, Yang ZS, Lu Y, Li SL, He JY, Wang C, Zhang L, Kong L, Sun JP, Yu X
Nature (2022) 604 pp. 771-778 [ DOI: doi:10.1038/s41586-022-04590-8 Pubmed: 35418677 ]
- Nanobody-35 (13 kDa, Protein from Lama glama)
- Adgrg2-beta-gs complex (134 kDa, Complex)
- Gs (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody-35 (Complex from Lama glama)
- Adhesion g-protein coupled receptor g2 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor g2,mcherry (77 kDa, Protein from Mus musculus)
Cryo-EM structure of the P395-bound GPBAR-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cfm
Q-score: 0.517
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature (2020) 587 pp. 499-504 [ Pubmed: 32698187 DOI: doi:10.1038/s41586-020-2569-1 ]
- Gpbar (Complex from Homo sapiens)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- P395 (Complex from synthetic construct)
- Gs (Complex from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- P395-bound gpbar-gs complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- 2-(ethylamino)-6-[3-(4-propan-2-ylphenyl)propanoyl]-7,8-dihydro-5h-pyrido[4,3-d]pyrimidine-4-carboxamide (395 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Cryo-EM structure of the INT-777-bound GPBAR-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cfn
Q-score: 0.53
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature (2020) 587 pp. 499-504 [ Pubmed: 32698187 DOI: doi:10.1038/s41586-020-2569-1 ]
- Int-777-bound gpbar-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein subunits (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- (2s,4r)-4-[(3r,5s,6r,7r,8r,9s,10s,12s,13r,14s,17r)-6-ethyl-10,13-dimethyl-3,7,12-tris(oxidanyl)-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methyl-pentanoic acid (450 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- G-protein coupled bile acid receptor 1 (Complex)
- Nanobody-35 (Complex from Homo sapiens)