Cryo-EM structure of GPR119-Gs-Nb35 complex with small molecule agonist MBX-2982
Resolution: 3.51 Å
EM Method: Single-particle
Fitted PDBs: 8vhf
Q-score: 0.449
Kim D-G, Liu W, Viner R, Cherezov V
To Be Published
- 2-[1-(5-ethylpyrimidin-2-yl)piperidin-4-yl]-4-[[4-(1,2,3,4-tetrazol-1-yl)phenoxy]methyl]-1,3-thiazole (448 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, hibit (40 kDa, Protein from Homo sapiens)
- The complex of gpr119-gs-nb35 (composite map) (177 kDa, Complex)
- Soluble cytochrome b562,glucose-dependent insulinotropic receptor,lgbit (67 kDa, Protein from Oplophorus gracilirostris)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- G-protein complex (93 kDa, Complex from Homo sapiens)
- Glucose-dependent insulinotropic receptor (67 kDa, Complex from Escherichia coli)
Cryo-EM structure of GPR119-Gs-Nb35 complex with small molecule agonist MBX-2982 (consensus map)
Kim D-G, Liu W, Viner R, Cherezov V
To Be Published
- Nanobody35 (Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Protein from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1,hibit (Protein from Homo sapiens)
- Soluble cytochrome b562,glucose-dependent insulinotropic receptor,lgbit (Protein from synthetic construct)
- Gpr119-gs-nb35 complex (177 kDa, Complex)
- G-protein complex (Complex from Homo sapiens)
- Glucose-dependent insulinotropic receptor (Complex from Escherichia coli)
Kim D-G, Liu W, Viner R, Cherezov V
To Be Published
- Nanobody35 (Protein from Lama glama)
- Gpr119-gs-nb35 complex (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Protein from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1,hibit (Protein from Homo sapiens)
- Soluble cytochrome b562,glucose-dependent insulinotropic receptor,lgbit (Protein from synthetic construct)
- G-protein complex (Complex from Homo sapiens)
- Glucose-dependent insulinotropic receptor (67 kDa, Complex from Escherichia coli)
Kim D-G, Liu W, Viner R, Cherezov V
To Be Published
- Gs-nb35 complex (110 kDa, Complex)
- Glucose-dependent insulinotropic receptor (Complex from Escherichia coli)
- G-protein complex (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-bindign protein g(i)/g(s)/g(t) subunit beta-1,hibit (Protein from Homo sapiens)
- Guanine nucleotide-bindign protein g(i)/g(s)/g(o) subunit gamma-2 (Protein from Homo sapiens)
- Nanobody35 (Protein from Lama glama)
- Gpr119-gs-nb35 complex (Complex)
- Guanine nucleotide-bindign protein g(s) subunit alpha isoforms short (Protein from Homo sapiens)
- Soluble cytochrome b562,glucose-dependent insulinotropic receptor,lgbit (Protein from synthetic construct)
Kim D-G, Liu W, Viner R, Cherezov V
To Be Published
- Heterotrimeric gs protein (Complex from Homo sapiens)
- Nanobody35 (Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Protein from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1,hibit (Protein from Homo sapiens)
- Oea-gpr119-gs-nb35 complex (177 kDa, Complex)
- Glucose-dependent insulinotropic receptor gpr119 (Complex from Escherichia coli)
- Soluble cytochrome b562,glucose-dependent insulinotropic receptor gpr119,lgbit (Protein from synthetic construct)
Structure of active state MC4R in complex with a potent ligand mimicking nanobody
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8qj2
Q-score: 0.431
Busch A, Jaakola V-P, Masiulis S
To Be Published
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody-35 (15 kDa, Protein from Lama glama)
- Soluble cytochrome b562,melanocortin receptor 4,red fluorescent protein drfp583 (82 kDa, Protein from Discosoma sp.)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Pn162 (12 kDa, Protein from Lama glama)
- Mc4r receptor in complex with gs protein heterotrimer, nanobody-35/confobody 35 and ligand-mimicking nanobody (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8pok
Q-score: 0.501
Kock Z, Schnelle K, Persechino M, Umbach S, Schihada H, Januliene D, Parey K, Pockes S, Kolb P, Dotsch V, Moller A, Hilger D, Bernhard F
Nat Commun (2024) 15 pp. 1831-1831 [ Pubmed: 38418462 DOI: doi:10.1038/s41467-024-46096-z ]
- Histamine h2 receptor (41 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Histamine (111 Da, Ligand)
- Histamine h2 receptor (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 and guanine nucleotide-binding protein subunit gamma (fragment) (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- H2 receptor coupled to heterotrimeric gs protein (Complex)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Human PTH1R in complex with PTHrP and Gs
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8flr
Q-score: 0.516
Cary BP, Gerrard EJ, Belousoff MJ, Fletcher MM, Jiang Y, Russell IC, Piper SJ, Wootten D, Sexton PM
Structure (2023) 31 pp. 668-676.e5 [ Pubmed: 37148874 DOI: doi:10.1016/j.str.2023.04.002 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (69 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Pthrp bound to the pth1r in complex with g protein (Complex from Homo sapiens)
- Nanobody35 (13 kDa, Protein from Lama glama)
- Pthrp[1-36] (4 kDa, Protein from Homo sapiens)
Human PTH1R in complex with Abaloparatide and Gs
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 8fls
Q-score: 0.473
Cary BP, Gerrard EJ, Belousoff MJ, Fletcher MM, Jiang Y, Russell IC, Piper SJ, Wootten D, Sexton PM
Structure (2023) 31 pp. 668-676.e5 [ Pubmed: 37148874 DOI: doi:10.1016/j.str.2023.04.002 ]
- Abaloparatide (3 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (69 kDa, Protein from Homo sapiens)
- Abaloparatide bound to the pth1r in complex with g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from Lama glama)
Human PTH1R in complex with M-PTH(1-14) and Gs
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8flt
Q-score: 0.528
Cary BP, Gerrard EJ, Belousoff MJ, Fletcher MM, Jiang Y, Russell IC, Piper SJ, Wootten D, Sexton PM
Structure (2023) 31 pp. 668-676.e5 [ Pubmed: 37148874 DOI: doi:10.1016/j.str.2023.04.002 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (69 kDa, Protein from Homo sapiens)
- Human pth1r in complex with m-pth(1-14) and gs (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- M-pth(1-14) (1 kDa, Protein from synthetic construct)
- Nanobody35 (13 kDa, Protein from Lama glama)