cryo-EM of human Gastric inhibitory polypeptide receptor GIPR bound to GIP
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 7ra3
Q-score: 0.436
Sun B, Willard FS, Feng D, Alsina-Fernandez J, Chen Q, Vieth M, Ho JD, Showalter AD, Stutsman C, Ding L, Suter TM, Dunbar JD, Carpenter JW, Mohammed FA, Aihara E, Brown RA, Bueno AB, Emmerson PJ, Moyers JS, Kobilka TS, Coghlan MP, Kobilka BK, Sloop KW
PNAS (2022) 119 pp. e2116506119-e2116506119 [ Pubmed: 35333651 DOI: doi:10.1073/pnas.2116506119 ]
- Gastric inhibitory polypeptide gip bound to gip receptor gipr (Complex from Homo sapiens)
- Single-chain variable fragment 16 (31 kDa, Protein from Mus musculus)
- Gastric inhibitory polypeptide (4 kDa, Protein from Homo sapiens)
- Gastric inhibitory polypeptide receptor (52 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i) subunit alpha-3,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Trimeric stimulatory g protein (Complex from Homo sapiens)
- Cryo-em of human gastric inhibitory polypeptide receptor gipr bound to gip, trimeric g protein complex and stabilizing antibodies (150 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
cryo-EM structure of human Gastric inhibitory polypeptide receptor GIPR bound to tirzepatide
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7rbt
Q-score: 0.479
Sun B, Willard FS, Feng D, Alsina-Fernandez J, Chen Q, Vieth M, Ho JD, Showalter AD, Stutsman C, Ding L, Suter TM, Dunbar JD, Carpenter JW, Mohammed FA, Aihara E, Brown RA, Bueno AB, Emmerson PJ, Moyers JS, Kobilka TS, Coghlan MP, Kobilka BK, Sloop KW
PNAS (2022) 119 pp. e2116506119-e2116506119 [ DOI: doi:10.1073/pnas.2116506119 Pubmed: 35333651 ]
- Cryo-em of human gastric inhibitory polypeptide receptor gipr bound to tirzepatide, trimeric g protein complex and stabilizing antibodies (150 kDa, Complex)
- Trimeric stimulatory g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Gastric inhibitory polypeptide receptor (52 kDa, Protein from Homo sapiens)
- Tirzepatide (4 kDa, Protein from Homo sapiens)
- 2-fluoro-4-[(1r)-6-methoxy-1-methyl-2-{(1s)-1-[4-(propan-2-yl)phenyl]ethyl}-1,2,3,4-tetrahydroisoquinolin-5-yl]-6-[(2-methylpropyl)amino]phenol (504 Da, Ligand)
- Single-chain variable fragment 16 (31 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Tirzepatide bound to gip receptor gipr (Complex from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
cryo-EM of human Glucagon-like peptide 1 receptor GLP-1R in apo form
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7rg9
Q-score: 0.476
Sun B, Willard FS, Feng D, Alsina-Fernandez J, Chen Q, Vieth M, Ho JD, Showalter AD, Stutsman C, Ding L, Suter TM, Dunbar JD, Carpenter JW, Mohammed FA, Aihara E, Brown RA, Bueno AB, Emmerson PJ, Moyers JS, Kobilka TS, Coghlan MP, Kobilka BK, Sloop KW
PNAS (2022) 119 pp. e2116506119-e2116506119 [ Pubmed: 35333651 DOI: doi:10.1073/pnas.2116506119 ]
- Single-chain variable fragment 16 (31 kDa, Protein from Mus musculus)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Glucagon-like peptide 1 receptor (51 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor in apo form (Complex from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Trimeric stimulatory g protein (Complex from Homo sapiens)
- Cryo-em of apo form human glucagon-like peptide 1 receptor glp-1r, trimeric g protein complex and stabilizing antibodies (150 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
cryo-EM of human Glucagon-like peptide 1 receptor GLP-1R bound to tirzepatide
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7rgp
Q-score: 0.484
Sun B, Willard FS, Feng D, Alsina-Fernandez J, Chen Q, Vieth M, Ho JD, Showalter AD, Stutsman C, Ding L, Suter TM, Dunbar JD, Carpenter JW, Mohammed FA, Aihara E, Brown RA, Bueno AB, Emmerson PJ, Moyers JS, Kobilka TS, Coghlan MP, Kobilka BK, Sloop KW
PNAS (2022) 119 pp. e2116506119-e2116506119 [ Pubmed: 35333651 DOI: doi:10.1073/pnas.2116506119 ]
- Single-chain variable fragment 16 (31 kDa, Protein from Mus musculus)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Glucagon-like peptide 1 receptor (51 kDa, Protein from Homo sapiens)
- Tirzepatide (4 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-3,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor bound to tirzepatide (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Trimeric stimulatory g protein (Complex from Homo sapiens)
- Cryo-em of human glucagon-like peptide 1 receptor glp-1r bound to tirzepatide, trimeric g protein complex and stabilizing antibodies (150 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
cryo-EM of human GLP-1R bound to non-peptide agonist LY3502970
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6xox
Q-score: 0.482
Kawai T, Sun B, Yoshino H, Feng D, Suzuki Y, Fukazawa M, Nagao S, Wainscott DB, Showalter AD, Droz BA, Kobilka TS, Coghlan MP, Willard FS, Kawabe Y, Kobilka BK, Sloop KW
PNAS (2020) 117 pp. 29959-29967 [ Pubmed: 33177239 DOI: doi:10.1073/pnas.2014879117 ]
- 3-[(1s,2s)-1-(5-[(4s)-2,2-dimethyloxan-4-yl]-2-{(4s)-2-(4-fluoro-3,5-dimethylphenyl)-3-[3-(4-fluoro-1-methyl-1h-indazol-5-yl)-2-oxo-2,3-dihydro-1h-imidazol-1-yl]-4-methyl-2,4,6,7-tetrahydro-5h-pyrazolo[4,3-c]pyridine-5-carbonyl}-1h-indol-1-yl)-2-methylcyclopropyl]-1,2,4-oxadiazol-5(4h)-one (882 Da, Ligand)
- Single-chain variable fragment 16 (Complex from Mus musculus)
- Cryo-em structure of the glucagon-like peptide-1 receptor in complex with g protein and a non-peptide agonist ly3502970 (150 kDa, Complex)
- Glucagon-like peptide 1 receptor (51 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Nanobody 35 (Complex from Lama glama)
- Alpha subunit of gs with n-terminus swapped with equivalent residues in gi,guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (43 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (Complex from Homo sapiens)
- Trimeric stimulatory g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Single-chain variable fragment scfv16 (27 kDa, Protein from Mus musculus)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6vcb
Q-score: 0.452
Bueno AB, Sun B, Willard FS, Feng D, Ho JD, Wainscott DB, Showalter AD, Vieth M, Chen Q, Stutsman C, Chau B, Ficorilli J, Agejas FJ, Cumming GR, Jimenez A, Rojo I, Kobilka TS, Kobilka BK, Sloop KW
Nat Chem Biol (2020) 16 pp. 1105-1110 [ DOI: doi:10.1038/s41589-020-0589-7 Pubmed: 32690941 ]
- 1-[(1r)-1-(2,6-dichloro-3-methoxyphenyl)ethyl]-6-{2-[(2r)-piperidin-2-yl]phenyl}-1h-benzimidazole (480 Da, Ligand)
- Glucagon-like peptide 1 receptor (51 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 (3 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Cryo-em structure of the glucagon-like peptide-1 receptor in complex with g protein, glp-1 peptide and a positive allosteric modulator (150 kDa, Complex)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor, glucagon-like peptide 1 (Complex from Homo sapiens)
Cryo-EM structure of the activated Glucagon-like peptide-1 receptor in complex with G protein
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 5vai
Q-score: 0.297
Zhang Y, Sun B, Feng D, Hu H, Chu M, Qu Q, Tarrasch JT, Li S, Sun Kobilka T, Kobilka BK, Skiniotis G
Nature (2017) 546 pp. 248-253 [ Pubmed: 28538729 DOI: doi:10.1038/nature22394 ]
- Glucagon-like peptide 1 (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Uncharacterized protein (52 kDa, Protein from Oryctolagus cuniculus)
- The activated glucagon-like peptide-1 receptor in complex with the heterotrimeric gs protein (150 kDa, Complex from Oryctolagus cuniculus)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)