Cryo-EM structure of the R399-bound GPBAR-Gs complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7xtq
Q-score: 0.456
Ma L, Yang F, Wu X, Mao C, Guo L, Miao T, Zang SK, Jiang X, Shen DD, Wei T, Zhou H, Wei Q, Li S, Shu Q, Feng S, Jiang C, Chu B, Du L, Sun JP, Yu X, Zhang Y, Zhang P
PNAS (2022) 119 pp. e2117054119-e2117054119 [ DOI: doi:10.1073/pnas.2117054119 Pubmed: 35858343 ]
- R399-bound gpbar-gs complex (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Gpbar (Complex from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Gs (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- [2-(2,5-dichloro-phenoxy)-pyridin-3-yl]-(3,4-dihydro-2h-quinolin-1-yl)-methanone (399 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nanobody-35 (Complex from synthetic construct)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
Cryo-EM structure of the P395-bound GPBAR-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cfm
Q-score: 0.517
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature (2020) 587 pp. 499-504 [ Pubmed: 32698187 DOI: doi:10.1038/s41586-020-2569-1 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Gpbar (Complex from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Gs (Complex from Homo sapiens)
- P395-bound gpbar-gs complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- 2-(ethylamino)-6-[3-(4-propan-2-ylphenyl)propanoyl]-7,8-dihydro-5h-pyrido[4,3-d]pyrimidine-4-carboxamide (395 Da, Ligand)
- P395 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Water (18 Da, Ligand)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
Cryo-EM structure of the INT-777-bound GPBAR-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cfn
Q-score: 0.53
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature (2020) 587 pp. 499-504 [ Pubmed: 32698187 DOI: doi:10.1038/s41586-020-2569-1 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Int-777-bound gpbar-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- G-protein coupled bile acid receptor 1 (Complex)
- Nanobody-35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein subunits (Complex from synthetic construct)
- Cholesterol (386 Da, Ligand)
- Water (18 Da, Ligand)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- (2s,4r)-4-[(3r,5s,6r,7r,8r,9s,10s,12s,13r,14s,17r)-6-ethyl-10,13-dimethyl-3,7,12-tris(oxidanyl)-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methyl-pentanoic acid (450 Da, Ligand)