Cryo-EM structure of GPR119-Gs-Nb35 complex with small molecule agonist MBX-2982
Resolution: 3.51 Å
EM Method: Single-particle
Fitted PDBs: 8vhf
Q-score: 0.449
Kim D-G, Liu W, Viner R, Cherezov V
To Be Published
- 2-[1-(5-ethylpyrimidin-2-yl)piperidin-4-yl]-4-[[4-(1,2,3,4-tetrazol-1-yl)phenoxy]methyl]-1,3-thiazole (448 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, hibit (40 kDa, Protein from Homo sapiens)
- The complex of gpr119-gs-nb35 (composite map) (177 kDa, Complex)
- Soluble cytochrome b562,glucose-dependent insulinotropic receptor,lgbit (67 kDa, Protein from Oplophorus gracilirostris)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- G-protein complex (93 kDa, Complex from Homo sapiens)
- Glucose-dependent insulinotropic receptor (67 kDa, Complex from Escherichia coli)
CryoEM structure of Gs-coupled GPBAR with small molecule agonist P395
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 9gyo
Q-score: 0.629
Castel J, Botzanowski T, Brooks I, Frechard A, Bey G, Schroeter M, Del nero E, Debaene F, Ciesielski F, Zeyer D, Cianferani S, Morales R
To Be Published
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- 2-(ethylamino)-6-[3-(4-propan-2-ylphenyl)propanoyl]-7,8-dihydro-5h-pyrido[4,3-d]pyrimidine-4-carboxamide (395 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (16 kDa, Protein from Lama glama)
- Complex of the tgr5 with gs protein heterotrimer, nanobody 35 and small molecule agonist p395 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Soluble cytochrome b562,g-protein coupled bile acid receptor 1 (50 kDa, Protein from Homo sapiens)
ADP-bound purinergic receptor 1 in complex with miniGs/q
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8wjx
Q-score: 0.49
To Be Published
- Gnas complex locus (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,p2y purinoceptor 1,soluble cytochrome b562,p2y purinoceptor 1 (76 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from Homo sapiens)
- Adp-bound purinergic receptor 1 in complex with minigs/q (Complex from Homo sapiens)
Structure of human class T GPCR TAS2R14-miniGs/gust complex with Aristolochic acid A.
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 8xql
Q-score: 0.469
Hu X, Ao W, Gao M, Wu L, Pei Y, Liu S, Wu Y, Zhao F, Sun Q, Liu J, Jiang L, Wang X, Li Y, Tan Q, Cheng J, Yang F, Yang C, Sun J, Hua T, Liu ZJ
Nature (2024) 631 pp. 459-466 [ Pubmed: 38776963 DOI: doi:10.1038/s41586-024-07569-9 ]
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Exo-alpha-sialidase,taste receptor type 2 member 14,lgbit (110 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (14 kDa, Protein from Homo sapiens)
- 8-methoxy-6-nitro-naphtho[1,2-e][1,3]benzodioxole-5-carboxylic acid (341 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Complex of tas2r14-minigsg with aristolochic acid a (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Structure 2 of human class T GPCR TAS2R14-miniGs/gust complex with Flufenamic acid.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8xqr
Q-score: 0.386
Hu X, Ao W, Gao M, Wu L, Pei Y, Liu S, Wu Y, Zhao F, Sun Q, Liu J, Jiang L, Wang X, Li Y, Tan Q, Cheng J, Yang F, Yang C, Sun J, Hua T, Liu ZJ
Nature (2024) 631 pp. 459-466 [ Pubmed: 38776963 DOI: doi:10.1038/s41586-024-07569-9 ]
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Exo-alpha-sialidase,taste receptor type 2 member 14,lgbit (110 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Structure 2 of human class t gpcr tas2r14-minigs/gust complex with flufenamic acid. (Complex from Homo sapiens)
- Nanobody 35 (14 kDa, Protein from Homo sapiens)
- 2-[[3-(trifluoromethyl)phenyl]amino] benzoic acid (281 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Cryo-EM structure of the amthamine-bound H2R-Gs complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8yut
Q-score: 0.529
Shen Q, Tang X, Wen X, Cheng S, Xiao P, Zang SK, Shen DD, Jiang L, Zheng Y, Zhang H, Xu H, Mao C, Zhang M, Hu W, Sun JP, Zhang Y, Chen Z
Adv Sci (2024) 11 pp. e2310120-e2310120 [ Pubmed: 38647423 DOI: doi:10.1002/advs.202310120 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Histamine h2 receptor (46 kDa, Protein from Homo sapiens)
- Amthamine-bound h2r-gs complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 5-(2-azanylethyl)-4-methyl-1,3-thiazol-2-amine (157 Da, Ligand)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
Human Amylin1 Receptor in Complex with Gs and human Calcitonin Gene-Related Peptide
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 9auc
Q-score: 0.503
Cao J, Belousoff MJ, Danev R, Christopoulos A, Wootten D, Sexton PM
Biochemistry (2024) 63 pp. 1089-1096 [ DOI: doi:10.1021/acs.biochem.4c00114 Pubmed: 38603770 ]
- Receptor activity-modifying protein 1 (17 kDa, Protein from Homo sapiens)
- Calcitonin gene-related peptide 1 (3 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Human amylin 1 receptor in complex with gs and human calcitonin gene-related peptide (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8pok
Q-score: 0.501
Kock Z, Schnelle K, Persechino M, Umbach S, Schihada H, Januliene D, Parey K, Pockes S, Kolb P, Dotsch V, Moller A, Hilger D, Bernhard F
Nat Commun (2024) 15 pp. 1831-1831 [ Pubmed: 38418462 DOI: doi:10.1038/s41467-024-46096-z ]
- Histamine h2 receptor (41 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Histamine (111 Da, Ligand)
- Histamine h2 receptor (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 and guanine nucleotide-binding protein subunit gamma (fragment) (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- H2 receptor coupled to heterotrimeric gs protein (Complex)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8smv
Q-score: 0.572
Hoppe N, Harrison S, Hwang SH, Chen Z, Karelina M, Deshpande I, Suomivuori CM, Palicharla VR, Berry SP, Tschaikner P, Regele D, Covey DF, Stefan E, Marks DS, Reiter JF, Dror RO, Evers AS, Mukhopadhyay S, Manglik A
Nat Struct Mol Biol (2024) 31 pp. 667-677 [ Pubmed: 38326651 DOI: doi:10.1038/s41594-024-01223-8 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 161, guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Gpr161 gs heterotrimeric complex with nb35 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 161 (59 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
Human Consensus Olfactory Receptor OR52c in Complex with Octanoic Acid (OCA) and G Protein
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 8hti
Q-score: 0.583
Choi C, Bae J, Kim S, Lee S, Kang H, Kim J, Bang I, Kim K, Huh WK, Seok C, Park H, Im W, Choi HJ
Nat Commun (2023) 14 pp. 8105-8105 [ Pubmed: 38062020 DOI: doi:10.1038/s41467-023-43983-9 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Consensus olfactory receptor or52c (36 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Complex of consensus olfactory receptor or52c in complex with octanoic acid, g protein and nanobody 35 (nb35) (140 kDa, Complex from Homo sapiens)
- Octanoic acid (caprylic acid) (144 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)