Cryo-EM structure of the fenoldopam-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7x2c
Q-score: 0.465
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ DOI: doi:10.1038/s41467-022-30929-w Pubmed: 35676276 ]
- Nanobody35 (Complex)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Nanobody35 (17 kDa, Protein from synthetic construct)
- Cryo-em structure of the fenoldopam-bound d1r-minigs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- (1r)-6-chloranyl-1-(4-hydroxyphenyl)-2,3,4,5-tetrahydro-1h-3-benzazepine-7,8-diol (305 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Gbeta-1gama-2 (Complex from Homo sapiens)
- The fenoldopam-bound d1r-minigs (Complex)
Cryo-EM structure of dopamine receptor 1 and mini-Gs complex with dopamine bound
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f0t
Q-score: 0.498
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ Pubmed: 35687690 DOI: doi:10.1126/sciadv.abo4158 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Nanobody35 (17 kDa, Protein from synthetic construct)
- Cryo-em structure of dopamine receptor 1 and mini-gs complex with dopamine bound (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
Cryo-EM structure of the GDP-bound dopamine receptor 1 and mini-Gs complex with Nb35
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 7f1o
Q-score: 0.506
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ Pubmed: 35687690 DOI: doi:10.1126/sciadv.abo4158 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Cryo-em structure of the gdp-bound dopamine receptor 1 and mini-gs complex with nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-Gs complex with Nb35
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 7f23
Q-score: 0.456
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ Pubmed: 35687690 DOI: doi:10.1126/sciadv.abo4158 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gtp-bound dopamine receptor 1 and mini-gs complex with nb35 (Complex from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
Cryo-EM structure of the tavapadon-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7x2d
Q-score: 0.495
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ Pubmed: 35676276 DOI: doi:10.1038/s41467-022-30929-w ]
- Tavapadon-bound d1r-minigs (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nanobody35 (17 kDa, Protein from synthetic construct)
- Cryo-em structure of the tavapadon-bound d1r-minigs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- 1,5-dimethyl-6-[2-methyl-4-[3-(trifluoromethyl)pyridin-2-yl]oxy-phenyl]pyrimidine-2,4-dione (391 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex)
Cryo-EM structure of the dopamine and LY3154207-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7x2f
Q-score: 0.471
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ Pubmed: 35676276 DOI: doi:10.1038/s41467-022-30929-w ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nanobody35 (17 kDa, Protein from synthetic construct)
- 2-[2,6-bis(chloranyl)phenyl]-1-[(1s,3r)-3-(hydroxymethyl)-1-methyl-5-(3-methyl-3-oxidanyl-butyl)-3,4-dihydro-1h-isoquinolin-2-yl]ethanone (450 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cryo-em structure of the dopamine-ly3154207-bound d1r-minigs complexthe (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Dopamine-ly3154207-bound d1r-minigs (Complex)
- Nanobody35 (Complex)