Cryo-EM structure of the amthamine-bound H2R-Gs complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8yut
Q-score: 0.529
Shen Q, Tang X, Wen X, Cheng S, Xiao P, Zang SK, Shen DD, Jiang L, Zheng Y, Zhang H, Xu H, Mao C, Zhang M, Hu W, Sun JP, Zhang Y, Chen Z
Adv Sci (2024) 11 pp. e2310120-e2310120 [ DOI: doi:10.1002/advs.202310120 Pubmed: 38647423 ]
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- 5-(2-azanylethyl)-4-methyl-1,3-thiazol-2-amine (157 Da, Ligand)
- Histamine h2 receptor (46 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Amthamine-bound h2r-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Serotonin 4 (5-HT4) receptor-Gs-Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7xt8
Q-score: 0.533
Huang S, Xu P, Shen DD, Simon IA, Mao C, Tan Y, Zhang H, Harpsoe K, Li H, Zhang Y, You C, Yu X, Jiang Y, Zhang Y, Gloriam DE, Xu HE
Mol Cell (2022) 82 pp. 2681-2695.e6 [ DOI: doi:10.1016/j.molcel.2022.05.031 Pubmed: 35714614 ]
- Palmitic acid (256 Da, Ligand)
- Serotonin (176 Da, Ligand)
- Soluble cytochrome b562,5-hydroxytryptamine receptor 4 (59 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Serotonin 4 (5-ht4) receptor-gs-nb35 complex (Complex from Homo sapiens)
Serotonin 6 (5-HT6) receptor-Gs-Nb35 complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7xtb
Q-score: 0.46
Huang S, Xu P, Shen DD, Simon IA, Mao C, Tan Y, Zhang H, Harpsoe K, Li H, Zhang Y, You C, Yu X, Jiang Y, Zhang Y, Gloriam DE, Xu HE
Mol Cell (2022) 82 pp. 2681-2695.e6 [ Pubmed: 35714614 DOI: doi:10.1016/j.molcel.2022.05.031 ]
- Serotonin 6 (5-ht6) receptor-gs-nb35 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Serotonin (176 Da, Ligand)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,5-hydroxytryptamine receptor 6 (63 kDa, Protein from Homo sapiens)
Serotonin 7 (5-HT7) receptor-Gs-Nb35 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7xtc
Q-score: 0.455
Huang S, Xu P, Shen DD, Simon IA, Mao C, Tan Y, Zhang H, Harpsoe K, Li H, Zhang Y, You C, Yu X, Jiang Y, Zhang Y, Gloriam DE, Xu HE
Mol Cell (2022) 82 pp. 2681-2695.e6 [ DOI: doi:10.1016/j.molcel.2022.05.031 Pubmed: 35714614 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Serotonin 7 (5-ht7) receptor-gs-nb35 complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 3-(2-azanylethyl)-1h-indole-5-carboxamide (203 Da, Ligand)
- Soluble cytochrome b562,5-hydroxytryptamine receptor 7 (64 kDa, Protein from Homo sapiens)
Structural insights into human brain gut peptide cholecystokinin receptors
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7xov
Q-score: -0.079
Ding Y, Zhang H, Liao YY, Chen LN, Ji SY, Qin J, Mao C, Shen DD, Lin L, Wang H, Zhang Y, Li XM
Cell Discov (2022) 8 pp. 55-55 [ DOI: doi:10.1038/s41421-022-00420-3 Pubmed: 35672283 ]
- Nanobody35 (Complex)
- Nanobody 35 (13 kDa, Protein from synthetic construct)
- 2-[2-[[4-(4-chloranyl-2,5-dimethoxy-phenyl)-5-(2-cyclohexylethyl)-1,3-thiazol-2-yl]carbamoyl]-5,7-dimethyl-indol-1-yl]ethanoic acid (610 Da, Ligand)
- Gs protein (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor1 (Complex)
- Cholecystokinin receptor1 (Complex from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Cryo-EM structure of a-MSH-MC4R-Gs_Nb35 complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f53
Q-score: 0.474
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Nanobody35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Complex of alpha-msh bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Alpha-melanocyte-stimulating hormones (1 kDa, Protein from synthetic construct)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Calcium ion (40 Da, Ligand)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Alpha-melanocyte-stimulating hormones (Complex)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
Cryo-EM structure of afamelanotide-MC4R-Gs_Nb35 complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f54
Q-score: 0.408
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Afamelanotide (Complex)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Nanobody35 (Complex from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Calcium ion (40 Da, Ligand)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Complex of afamelanotide bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
Cryo-EM structure of bremelanotide-MC4R-Gs_Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f55
Q-score: 0.411
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Bremelanotide (1 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Complex of bremelanotide bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Nanobody35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Bremelanotide (Complex)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
Cryo-EM structure of THIQ-MC4R-Gs_Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f58
Q-score: 0.516
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Nanobody35 (Complex)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Melanocrotin-4 receptor (Complex from Homo sapiens)
- Complex of thiq bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- (3r)-n-[(2r)-3-(4-chlorophenyl)-1-[4-cyclohexyl-4-(1,2,4-triazol-1-ylmethyl)piperidin-1-yl]-1-oxidanylidene-propan-2-yl]-1,2,3,4-tetrahydroisoquinoline-3-carboxamide (589 Da, Ligand)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
Cryo-EM structure of the PGE2-bound EP2-Gs complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7cx2
Q-score: 0.536
Qu C, Mao C, Xiao P, Shen Q, Zhong YN, Yang F, Shen DD, Tao X, Zhang H, Yan X, Zhao RJ, He J, Guan Y, Zhang C, Hou G, Zhang PJ, Hou G, Li Z, Yu X, Chai RJ, Guan YF, Sun JP, Zhang Y
Sci Adv (2021) 7 [ Pubmed: 33811074 DOI: doi:10.1126/sciadv.abf1268 ]
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ep2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Prostaglandin e2 receptor ep2 subtype (39 kDa, Protein from Homo sapiens)
- (z)-7-[(1r,2r,3r)-3-hydroxy-2-[(e,3s)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid (352 Da, Ligand)
- Gs (Complex from Homo sapiens)
- Nb35 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Pge2-bound ep2-gs complex (Complex)