Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 8f0j
Q-score: 0.621
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ Pubmed: 37537379 DOI: doi:10.1038/s41589-023-01393-4 ]
- Palmitic acid (256 Da, Ligand)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- (2s)-2-{[(1r)-1-hydroxyhexadecyl]oxy}-3-{[(1r)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate (766 Da, Ligand)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Water (18 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Pramlintide analogue san45 (3 kDa, Protein from Homo sapiens)
- Calcitonin receptor in complex with gs protein and san45 peptide (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
Resolution: 1.9 Å
EM Method: Single-particle
Fitted PDBs: 8f0k
Q-score: 0.564
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ Pubmed: 37537379 DOI: doi:10.1038/s41589-023-01393-4 ]
- Palmitic acid (256 Da, Ligand)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- (2s)-2-{[(1r)-1-hydroxyhexadecyl]oxy}-3-{[(1r)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate (766 Da, Ligand)
- San385 (3 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Human amylin 3 receptor in complex with gs protein and san385 peptide (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8f2a
Q-score: 0.556
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ Pubmed: 37537379 DOI: doi:10.1038/s41589-023-01393-4 ]
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Pramlintide analogue san385 (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Human amylin 3 receptor in complex with gs protein and san385 peptide in another conformation (cluster 5) (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 8f2b
Q-score: 0.556
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ Pubmed: 37537379 DOI: doi:10.1038/s41589-023-01393-4 ]
- Palmitic acid (256 Da, Ligand)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- (2s)-2-{[(1r)-1-hydroxyhexadecyl]oxy}-3-{[(1r)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate (766 Da, Ligand)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Water (18 Da, Ligand)
- Amylin 3 receptor in complex with gs protein and san45 peptide (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Pramlintide analogue san45 (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
Human PTH1R in complex with PTH(1-34) and Gs
Resolution: 2.55 Å
EM Method: Single-particle
Fitted PDBs: 8flq
Q-score: 0.538
Cary BP, Gerrard EJ, Belousoff MJ, Fletcher MM, Jiang Y, Russell IC, Piper SJ, Wootten D, Sexton PM
Structure (2023) 31 pp. 668-676.e5 [ Pubmed: 37148874 DOI: doi:10.1016/j.str.2023.04.002 ]
- Pth bound to the pth1r in complex with g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Parathyroid hormone (4 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (69 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from Lama glama)
Human PTH1R in complex with PTHrP and Gs
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8flr
Q-score: 0.516
Cary BP, Gerrard EJ, Belousoff MJ, Fletcher MM, Jiang Y, Russell IC, Piper SJ, Wootten D, Sexton PM
Structure (2023) 31 pp. 668-676.e5 [ Pubmed: 37148874 DOI: doi:10.1016/j.str.2023.04.002 ]
- Pthrp[1-36] (4 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (69 kDa, Protein from Homo sapiens)
- Pthrp bound to the pth1r in complex with g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from Lama glama)
Human PTH1R in complex with Abaloparatide and Gs
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 8fls
Q-score: 0.473
Cary BP, Gerrard EJ, Belousoff MJ, Fletcher MM, Jiang Y, Russell IC, Piper SJ, Wootten D, Sexton PM
Structure (2023) 31 pp. 668-676.e5 [ Pubmed: 37148874 DOI: doi:10.1016/j.str.2023.04.002 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (69 kDa, Protein from Homo sapiens)
- Abaloparatide (3 kDa, Protein from synthetic construct)
- Abaloparatide bound to the pth1r in complex with g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from Lama glama)
Human PTH1R in complex with M-PTH(1-14) and Gs
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8flt
Q-score: 0.528
Cary BP, Gerrard EJ, Belousoff MJ, Fletcher MM, Jiang Y, Russell IC, Piper SJ, Wootten D, Sexton PM
Structure (2023) 31 pp. 668-676.e5 [ Pubmed: 37148874 DOI: doi:10.1016/j.str.2023.04.002 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (69 kDa, Protein from Homo sapiens)
- M-pth(1-14) (1 kDa, Protein from synthetic construct)
- Human pth1r in complex with m-pth(1-14) and gs (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from Lama glama)
Human PTH1R in complex with LA-PTH and Gs
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8flu
Q-score: 0.463
Cary BP, Gerrard EJ, Belousoff MJ, Fletcher MM, Jiang Y, Russell IC, Piper SJ, Wootten D, Sexton PM
Structure (2023) 31 pp. 668-676.e5 [ Pubmed: 37148874 DOI: doi:10.1016/j.str.2023.04.002 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Human pth1r in complex with la-pth and gs (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Long-acting pth (4 kDa, Protein from synthetic construct)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (69 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from Lama glama)
Cryo-EM structure of the PAC1R-PACAP27-Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8e3x
Q-score: 0.543
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ DOI: doi:10.1038/s41467-022-34629-3 Pubmed: 36385145 ]
- Nanobody35 (15 kDa, Protein from Lama glama)
- Pituitary adenylate cyclase activating polypeptide-27 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide type i receptor (55 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, pituitary adenylate cyclase-activating polypeptide type i receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide (3 kDa, Protein from Homo sapiens)
- Gs coupled pituitary adenylate cyclase-activating polypeptide type i receptor complex with pacap27 peptide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)