Cryo-EM structure of Lys05 bound GPR30-Gq complex structure
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8xof
Q-score: 0.551
Liu H, Guo S, Dai A, Xu P, Li X, Huang S, He X, Wu K, Zhang X, Yang D, Xie X, Xu HE
Cell Res (2024) 34 pp. 530-533 [ DOI: doi:10.1038/s41422-024-00963-y Pubmed: 38744981 ]
- G-protein coupled estrogen receptor 1 (42 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- G protein subunit q (41 kDa, Protein from Homo sapiens)
- Gpr30-gq (Complex from Homo sapiens)
- Gpr30-gq complex (Complex)
- Lys01 (440 Da, Ligand)
Structure of A2BR bound to synthetic agonists BAY 60-6583
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8hdo
Q-score: 0.497
Cai H, Xu Y, Guo S, He X, Sun J, Li X, Li C, Yin W, Cheng X, Jiang H, Xu HE, Xie X, Jiang Y
Cell Discov (2022) 8 pp. 140-140 [ Pubmed: 36575181 DOI: doi:10.1038/s41421-022-00503-1 ]
- Adenosine a2b receptor (36 kDa, Protein from Homo sapiens)
- A2br-g protein-bay 60-6583 complex (160 kDa, Complex from Homo sapiens)
- 2-[6-azanyl-3,5-dicyano-4-[4-(cyclopropylmethoxy)phenyl]pyridin-2-yl]sulfanylethanamide (379 Da, Ligand)
- Chimeric minigs (29 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody 35 (13 kDa, Protein from Camelus bactrianus)
Structure of A2BR bound to endogenous agonists adenosine
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8hdp
Q-score: 0.428
Cai H, Xu Y, Guo S, He X, Sun J, Li X, Li C, Yin W, Cheng X, Jiang H, Xu HE, Xie X, Jiang Y
Cell Discov (2022) 8 pp. 140-140 [ Pubmed: 36575181 DOI: doi:10.1038/s41421-022-00503-1 ]
- Adenosine a2b receptor (36 kDa, Protein from Homo sapiens)
- Chimeric minigs (29 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody 35 (13 kDa, Protein from Camelus bactrianus)
- Adenosine (267 Da, Ligand)
- A2br-g protein-adenosine complex (160 kDa, Complex from Homo sapiens)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7xz5
Q-score: 0.514
Xu P, Huang S, Guo S, Yun Y, Cheng X, He X, Cai P, Lan Y, Zhou H, Jiang H, Jiang Y, Xie X, Xu HE
Nat Struct Mol Biol (2022) 29 pp. 863-870 [ DOI: doi:10.1038/s41594-022-00816-5 Pubmed: 35970999 ]
- Gpr119-gs-nb35-lpc complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nb35 (Complex)
- (4r,7r,18e)-4,7-dihydroxy-n,n,n-trimethyl-10-oxo-3,5,9-trioxa-4-phosphaheptacos-18-en-1-aminium 4-oxide (522 Da, Ligand)
- Gpr119-gs (Complex from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,glucose-dependent insulinotropic receptor (53 kDa, Protein from Homo sapiens)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7xz6
Q-score: 0.552
Xu P, Huang S, Guo S, Yun Y, Cheng X, He X, Cai P, Lan Y, Zhou H, Jiang H, Jiang Y, Xie X, Xu HE
Nat Struct Mol Biol (2022) 29 pp. 863-870 [ DOI: doi:10.1038/s41594-022-00816-5 Pubmed: 35970999 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nb35 (Complex)
- Water (18 Da, Ligand)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Gpr119-gs (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Propan-2-yl 4-[1-(2-fluoranyl-4-methylsulfonyl-phenyl)pyrazolo[3,4-d]pyrimidin-4-yl]oxypiperidine-1-carboxylate (477 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,glucose-dependent insulinotropic receptor (53 kDa, Protein from Homo sapiens)
- Gpr119-gs-nb35-apd668 complex (Complex from Homo sapiens)
Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7wui
Q-score: 0.418
Xiao P, Guo S, Wen X, He QT, Lin H, Huang SM, Gou L, Zhang C, Yang Z, Zhong YN, Yang CC, Li Y, Gong Z, Tao XN, Yang ZS, Lu Y, Li SL, He JY, Wang C, Zhang L, Kong L, Sun JP, Yu X
Nature (2022) 604 pp. 771-778 [ DOI: doi:10.1038/s41586-022-04590-8 Pubmed: 35418677 ]
- Cryo-em structure of the adgrg2-fl-ip15-gs complex (253 MDa, Complex)
- Nanobody-35 (13 kDa, Protein from Lama glama)
- Gs (Complex from Homo sapiens)
- Ip15 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Adhesion g-protein coupled receptor g2,mcherry (126 kDa, Protein from Mus musculus)
- Ip15 (1 kDa, Protein from synthetic construct)
- Nanobody-35 (Complex from Lama glama)
- Adhesion g-protein coupled receptor g2 (Complex from Mus musculus)
- Mini-gs (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from synthetic construct)
Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7wuj
Q-score: 0.474
Xiao P, Guo S, Wen X, He QT, Lin H, Huang SM, Gou L, Zhang C, Yang Z, Zhong YN, Yang CC, Li Y, Gong Z, Tao XN, Yang ZS, Lu Y, Li SL, He JY, Wang C, Zhang L, Kong L, Sun JP, Yu X
Nature (2022) 604 pp. 771-778 [ DOI: doi:10.1038/s41586-022-04590-8 Pubmed: 35418677 ]
- Nanobody-35 (13 kDa, Protein from Lama glama)
- Gs (Complex from Homo sapiens)
- Adhesion g-protein coupled receptor g4,uncharacterized protein (71 kDa, Protein from Psychromonas sp. B3M02)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody-35 (Complex from Lama glama)
- Mini-gs (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from synthetic construct)
- Cryo-em structure of the adgr4-beta-gs complex (Complex)
Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7wuq
Q-score: 0.465
Xiao P, Guo S, Wen X, He QT, Lin H, Huang SM, Gou L, Zhang C, Yang Z, Zhong YN, Yang CC, Li Y, Gong Z, Tao XN, Yang ZS, Lu Y, Li SL, He JY, Wang C, Zhang L, Kong L, Sun JP, Yu X
Nature (2022) 604 pp. 771-778 [ DOI: doi:10.1038/s41586-022-04590-8 Pubmed: 35418677 ]
- Nanobody-35 (13 kDa, Protein from Lama glama)
- Adgrg2-beta-gs complex (134 kDa, Complex)
- Gs (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody-35 (Complex from Lama glama)
- Adhesion g-protein coupled receptor g2 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor g2,mcherry (77 kDa, Protein from Mus musculus)
Cryo-EM structure of the P395-bound GPBAR-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cfm
Q-score: 0.517
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature (2020) 587 pp. 499-504 [ Pubmed: 32698187 DOI: doi:10.1038/s41586-020-2569-1 ]
- Gpbar (Complex from Homo sapiens)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- P395 (Complex from synthetic construct)
- Gs (Complex from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- P395-bound gpbar-gs complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- 2-(ethylamino)-6-[3-(4-propan-2-ylphenyl)propanoyl]-7,8-dihydro-5h-pyrido[4,3-d]pyrimidine-4-carboxamide (395 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Cryo-EM structure of the INT-777-bound GPBAR-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cfn
Q-score: 0.53
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature (2020) 587 pp. 499-504 [ Pubmed: 32698187 DOI: doi:10.1038/s41586-020-2569-1 ]
- Int-777-bound gpbar-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein subunits (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- (2s,4r)-4-[(3r,5s,6r,7r,8r,9s,10s,12s,13r,14s,17r)-6-ethyl-10,13-dimethyl-3,7,12-tris(oxidanyl)-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methyl-pentanoic acid (450 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- G-protein coupled bile acid receptor 1 (Complex)
- Nanobody-35 (Complex from Homo sapiens)