Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 7piu
Q-score: 0.6
Heyder NA, Kleinau G, Speck D, Schmidt A, Paisdzior S, Szczepek M, Bauer B, Koch A, Gallandi M, Kwiatkowski D, Burger J, Mielke T, Beck-Sickinger AG, Hildebrand PW, Spahn CMT, Hilger D, Schacherl M, Biebermann H, Hilal T, Kuhnen P, Kobilka BK, Scheerer P
Cell Res (2021) 31 pp. 1176-1189 [ DOI: doi:10.1038/s41422-021-00569-8 Pubmed: 34561620 ]
- Calcium ion (40 Da, Ligand)
- Camelid antibody fragment - nanobody 35 (14 kDa, Protein from Lama glama)
- Melanocortin receptor 4 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Ternary complex of setmelanotide-activated melanocortin 4 receptor with heterotrimeric gs, further stabilized by addition of nanobody 35 (144 MDa, Complex from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Setmelanotide (other names rm-493; bim-22493; irc-022493; imcivree) (1 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 7piv
Q-score: 0.539
Heyder NA, Kleinau G, Speck D, Schmidt A, Paisdzior S, Szczepek M, Bauer B, Koch A, Gallandi M, Kwiatkowski D, Burger J, Mielke T, Beck-Sickinger AG, Hildebrand PW, Spahn CMT, Hilger D, Schacherl M, Biebermann H, Hilal T, Kuhnen P, Kobilka BK, Scheerer P
Cell Res (2021) 31 pp. 1176-1189 [ DOI: doi:10.1038/s41422-021-00569-8 Pubmed: 34561620 ]
- Ndp-alpha-msh (other names afamelanotide; scenesse) (1 kDa, Protein from synthetic construct)
- Calcium ion (40 Da, Ligand)
- Melanocortin receptor 4 (38 kDa, Protein from Homo sapiens)
- Ternary complex of the ndp-alpha-msh activated melanocortin 4 receptor (mc4r) gs protein complex stabilized by nanobody 35 (144 MDa, Complex from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Camelid antibody vhh fragment - nanobody 35 (14 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
Cryo-EM structure of the activated Glucagon-like peptide-1 receptor in complex with G protein
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 5vai
Q-score: 0.297
Zhang Y, Sun B, Feng D, Hu H, Chu M, Qu Q, Tarrasch JT, Li S, Sun Kobilka T, Kobilka BK, Skiniotis G
Nature (2017) 546 pp. 248-253 [ DOI: doi:10.1038/nature22394 Pubmed: 28538729 ]
- The activated glucagon-like peptide-1 receptor in complex with the heterotrimeric gs protein (150 kDa, Complex from Oryctolagus cuniculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Uncharacterized protein (52 kDa, Protein from Oryctolagus cuniculus)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Glucagon-like peptide 1 (3 kDa, Protein from Homo sapiens)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 5uz7
Q-score: 0.413
Liang YL, Khoshouei M, Radjainia M, Zhang Y, Glukhova A, Tarrasch J, Thal DM, Furness SGB, Christopoulos G, Coudrat T, Danev R, Baumeister W, Miller LJ, Christopoulos A, Kobilka BK, Wootten D, Skiniotis G, Sexton PM
Nature (2017) 546 pp. 118-123 [ Pubmed: 28437792 DOI: doi:10.1038/nature22327 ]
- Nanobody 35 (15 kDa, Protein from Lama Glama)
- Complex of a full-length, active-state calcitonin receptor with peptide ligand, heterotrimeric gs protein and nano body 35. (150 kDa, Complex from Homo sapiens)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)