Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8smv
Q-score: 0.572
Hoppe N, Harrison S, Hwang SH, Chen Z, Karelina M, Deshpande I, Suomivuori CM, Palicharla VR, Berry SP, Tschaikner P, Regele D, Covey DF, Stefan E, Marks DS, Reiter JF, Dror RO, Evers AS, Mukhopadhyay S, Manglik A
Nat Struct Mol Biol (2024) 31 pp. 667-677 [ DOI: doi:10.1038/s41594-024-01223-8 Pubmed: 38326651 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 161, guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Gpr161 gs heterotrimeric complex with nb35 (Complex)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- G-protein coupled receptor 161 (59 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
Native human TSH bound to human Thyrotropin receptor in complex with miniGs399 (composite structure)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7t9i
Q-score: 0.375
Faust B, Billesbolle CB, Suomivuori CM, Singh I, Zhang K, Hoppe N, Pinto AFM, Diedrich JK, Muftuoglu Y, Szkudlinski MW, Saghatelian A, Dror RO, Cheng Y, Manglik A
Nature (2022) 609 pp. 846-853 [ Pubmed: 35940205 DOI: doi:10.1038/s41586-022-05159-1 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Thyrotropin receptor (79 kDa, Protein from Homo sapiens)
- Native human tsh bound to human thyrotropin receptor in complex with minigs399 (composite structure) (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Thyrotropin subunit beta (12 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Glycoprotein hormones alpha chain (10 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7t9n
Q-score: 0.495
Faust B, Billesbolle CB, Suomivuori CM, Singh I, Zhang K, Hoppe N, Pinto AFM, Diedrich JK, Muftuoglu Y, Szkudlinski MW, Saghatelian A, Dror RO, Cheng Y, Manglik A
Nature (2022) 609 pp. 846-853 [ Pubmed: 35940205 DOI: doi:10.1038/s41586-022-05159-1 ]
- M22 fab heavy chain (27 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
- (2s)-3-hydroxypropane-1,2-diyl dihexadecanoate (568 Da, Ligand)
- M22 fab light chain (25 kDa, Protein from Homo sapiens)
- Thyrotropin receptor (79 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- M22 agonist autoantibody bound to human thyrotropin receptor in complex with minigs399 (composite structure) (Complex)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7utz
Q-score: 0.52
Faust B, Billesbolle CB, Suomivuori CM, Singh I, Zhang K, Hoppe N, Pinto AFM, Diedrich JK, Muftuoglu Y, Szkudlinski MW, Saghatelian A, Dror RO, Cheng Y, Manglik A
Nature (2022) 609 pp. 846-853 [ Pubmed: 35940205 DOI: doi:10.1038/s41586-022-05159-1 ]
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
- (2s)-3-hydroxypropane-1,2-diyl dihexadecanoate (568 Da, Ligand)
- Thyrotropin receptor (79 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Glycoprotein hormones alpha chain analog tr1402 (10 kDa, Protein from Homo sapiens)
- Human tsh analog tr1402 bound to human thyrotropin receptor in complex with minigs399 (composite structure) (Complex from Homo sapiens)
- Thyrotropin subunit beta (12 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
Substance P bound to active human neurokinin 1 receptor in complex with miniGs/q70
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7rmg
Q-score: 0.532
Harris JA, Faust B, Gondin AB, Damgen MA, Suomivuori CM, Veldhuis NA, Cheng Y, Dror RO, Thal DM, Manglik A
Nat Chem Biol (2022) 18 pp. 109-115 [ DOI: doi:10.1038/s41589-021-00890-8 Pubmed: 34711980 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1/gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Substance-p receptor (47 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, with certain residues mutated to match guanine nucleotide-binding protein g(q) subunit (26 kDa, Protein from Homo sapiens)
- Substance p (1 kDa, Protein from Homo sapiens)
- Substance-p receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s)/g(q) subunit alpha hybrid (Complex from Homo sapiens)
- Substance p bound to neurokinin 1 receptor-minigs/q70 complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Substance p (Complex from Homo sapiens)
Substance P bound to active human neurokinin 1 receptor in complex with miniGs399
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7rmh
Q-score: 0.492
Harris JA, Faust B, Gondin AB, Damgen MA, Suomivuori CM, Veldhuis NA, Cheng Y, Dror RO, Thal DM, Manglik A
Nat Chem Biol (2022) 18 pp. 109-115 [ Pubmed: 34711980 DOI: doi:10.1038/s41589-021-00890-8 ]
- Minigs399 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Substance-p receptor (Complex from Homo sapiens)
- Substance p (1 kDa, Protein from Homo sapiens)
- Substance p (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1/gamma-2 (Complex from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Nanobody 35 (Complex from Lama glama)
- Substance-p receptor (47 kDa, Protein from Homo sapiens)
- Substance p bound to neurokinin1 receptor-minigs399 complex (Complex)
SP6-11 biased agonist bound to active human neurokinin 1 receptor in complex with miniGs/q70
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7rmi
Q-score: 0.458
Harris JA, Faust B, Gondin AB, Damgen MA, Suomivuori CM, Veldhuis NA, Cheng Y, Dror RO, Thal DM, Manglik A
Nat Chem Biol (2022) 18 pp. 109-115 [ DOI: doi:10.1038/s41589-021-00890-8 Pubmed: 34711980 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1/gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Substance-p receptor (47 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, with certain residues mutated to match guanine nucleotide-binding protein g(q) subunit (26 kDa, Protein from Homo sapiens)
- Substance-p receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s)/g(q) subunit alpha hybrid (Complex from Homo sapiens)
- Substance p bound to neurokinin 1 receptor-minigs/q70 complex (Complex)
- Substance p 6-11 (739 Da, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Substance p 6-11 (Complex from synthetic construct)