Human proton sensing receptor GPR68 in complex with miniGs
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 9bhm
Q-score: 0.456
Howard MK, Hoppe N, Huang XP, Mitrovic D, Billesbolle CB, Macdonald CB, Mehrotra E, Rockefeller Grimes P, Trinidad DD, Delemotte L, English JG, Coyote-Maestas W, Manglik A
Cell (2025) 188 pp. 671- [ Pubmed: 39753132 DOI: doi:10.1016/j.cell.2024.11.036 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Ovarian cancer g-protein coupled receptor 1 (42 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Complex of gpr68 bound to gs heterotrimer (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
Consensus olfactory receptor consOR2 bound to S-carvone and in complex with mini-Gs trimeric protein
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8uy0
Q-score: 0.489
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ Pubmed: 39478229 DOI: doi:10.1038/s41586-024-08126-0 ]
- Consor2 (35 kDa, Protein from synthetic construct)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Minigs399 (30 kDa, Protein from Homo sapiens)
- (5s)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one (150 Da, Ligand)
- Consor2-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8smv
Q-score: 0.572
Hoppe N, Harrison S, Hwang SH, Chen Z, Karelina M, Deshpande I, Suomivuori CM, Palicharla VR, Berry SP, Tschaikner P, Regele D, Covey DF, Stefan E, Marks DS, Reiter JF, Dror RO, Evers AS, Mukhopadhyay S, Manglik A
Nat Struct Mol Biol (2024) 31 pp. 667-677 [ DOI: doi:10.1038/s41594-024-01223-8 Pubmed: 38326651 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Gpr161 gs heterotrimeric complex with nb35 (Complex)
- G-protein coupled receptor 161 (59 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 161, guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Human olfactory receptor OR51E2 bound to propionate in complex with miniGs399
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8f76
Q-score: 0.52
Billesbolle CB, de March CA, van der Velden WJC, Ma N, Tewari J, Del Torrent CL, Li L, Faust B, Vaidehi N, Matsunami H, Manglik A
Nature (2023) 615 pp. 742-749 [ DOI: doi:10.1038/s41586-023-05798-y Pubmed: 36922591 ]
- Or51e2-gs complex (100 kDa, Complex from Homo sapiens)
- Propanoic acid (74 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Olfactory receptor 51e2 (36 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
Native human TSH bound to human Thyrotropin receptor in complex with miniGs399 (composite structure)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7t9i
Q-score: 0.375
Faust B, Billesbolle CB, Suomivuori CM, Singh I, Zhang K, Hoppe N, Pinto AFM, Diedrich JK, Muftuoglu Y, Szkudlinski MW, Saghatelian A, Dror RO, Cheng Y, Manglik A
Nature (2022) 609 pp. 846-853 [ DOI: doi:10.1038/s41586-022-05159-1 Pubmed: 35940205 ]
- Native human tsh bound to human thyrotropin receptor in complex with minigs399 (composite structure) (Complex from Homo sapiens)
- Thyrotropin receptor (79 kDa, Protein from Homo sapiens)
- Glycoprotein hormones alpha chain (10 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Thyrotropin subunit beta (12 kDa, Protein from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7t9n
Q-score: 0.495
Faust B, Billesbolle CB, Suomivuori CM, Singh I, Zhang K, Hoppe N, Pinto AFM, Diedrich JK, Muftuoglu Y, Szkudlinski MW, Saghatelian A, Dror RO, Cheng Y, Manglik A
Nature (2022) 609 pp. 846-853 [ DOI: doi:10.1038/s41586-022-05159-1 Pubmed: 35940205 ]
- Thyrotropin receptor (79 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- M22 agonist autoantibody bound to human thyrotropin receptor in complex with minigs399 (composite structure) (Complex)
- M22 fab light chain (25 kDa, Protein from Homo sapiens)
- M22 fab heavy chain (27 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- (2s)-3-hydroxypropane-1,2-diyl dihexadecanoate (568 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7utz
Q-score: 0.52
Faust B, Billesbolle CB, Suomivuori CM, Singh I, Zhang K, Hoppe N, Pinto AFM, Diedrich JK, Muftuoglu Y, Szkudlinski MW, Saghatelian A, Dror RO, Cheng Y, Manglik A
Nature (2022) 609 pp. 846-853 [ DOI: doi:10.1038/s41586-022-05159-1 Pubmed: 35940205 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein hormones alpha chain analog tr1402 (10 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- (2s)-3-hydroxypropane-1,2-diyl dihexadecanoate (568 Da, Ligand)
- Human tsh analog tr1402 bound to human thyrotropin receptor in complex with minigs399 (composite structure) (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Thyrotropin subunit beta (12 kDa, Protein from Homo sapiens)
- Thyrotropin receptor (79 kDa, Protein from Homo sapiens)