Cryo-EM structure of the PEA-bound mTAAR1-Gs complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8wc6
Q-score: 0.542
Shang P, Rong N, Jiang JJ, Cheng J, Zhang MH, Kang D, Qi L, Guo L, Yang GM, Liu Q, Zhou Z, Li XB, Zhu KK, Meng QB, Han X, Yan W, Kong Y, Yang L, Wang X, Lei D, Feng X, Liu X, Yu X, Wang Y, Li Q, Shao ZH, Yang F, Sun JP
Cell (2023) 186 pp. 5347-5362.e24 [ DOI: doi:10.1016/j.cell.2023.10.014 Pubmed: 37963465 ]
- Trace amine-associated receptor 1 (37 kDa, Protein from Mus musculus)
- Cryo-em structure of the pea-bound mtaar1-gs complex (Complex from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- 2-phenylethylamine (122 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (42 kDa, Protein from Homo sapiens)
Cryo-EM structure of the DMCHA-bound mTAAR9-Gs complex
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 8itf
Q-score: 0.491
Guo L, Cheng J, Lian S, Liu Q, Lu Y, Zheng Y, Zhu K, Zhang M, Kong Y, Zhang C, Rong N, Zhuang Y, Fang G, Jiang J, Zhang T, Han X, Liu Z, Xia M, Liu S, Zhang L, Liberles SD, Yu X, Xu Y, Yang F, Li Q, Sun JP
Nature (2023) 618 pp. 193-200 [ Pubmed: 37225986 DOI: doi:10.1038/s41586-023-06106-4 ]
- ~{n},~{n}-dimethylcyclohexanamine (127 Da, Ligand)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Cryo-em structure of the dmcha-bound mtaar9-gs complex (Complex from Mus musculus)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Trace amine-associated receptor 9 (38 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (42 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class T GPCR in active state
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 7xp6
Q-score: 0.415
Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ, Hua T
Science (2022) 377 pp. 1298-1304 [ DOI: doi:10.1126/science.abo1633 Pubmed: 36108005 ]
- Complex of gpcr and g protein (Complex from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
- Strychnine (334 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Endoglucanase h,taste receptor type 2 member 46,endoglucanase h,taste receptor type 2 member 46,bitter taste receptor t2r46 (91 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Serotonin 4 (5-HT4) receptor-Gs-Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7xt8
Q-score: 0.533
Huang S, Xu P, Shen DD, Simon IA, Mao C, Tan Y, Zhang H, Harpsoe K, Li H, Zhang Y, You C, Yu X, Jiang Y, Zhang Y, Gloriam DE, Xu HE
Mol Cell (2022) 82 pp. 2681-2695.e6 [ Pubmed: 35714614 DOI: doi:10.1016/j.molcel.2022.05.031 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Serotonin 4 (5-ht4) receptor-gs-nb35 complex (Complex from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Soluble cytochrome b562,5-hydroxytryptamine receptor 4 (59 kDa, Protein from Homo sapiens)
- Serotonin (176 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Serotonin 6 (5-HT6) receptor-Gs-Nb35 complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7xtb
Q-score: 0.46
Huang S, Xu P, Shen DD, Simon IA, Mao C, Tan Y, Zhang H, Harpsoe K, Li H, Zhang Y, You C, Yu X, Jiang Y, Zhang Y, Gloriam DE, Xu HE
Mol Cell (2022) 82 pp. 2681-2695.e6 [ Pubmed: 35714614 DOI: doi:10.1016/j.molcel.2022.05.031 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Serotonin 6 (5-ht6) receptor-gs-nb35 complex (Complex from Homo sapiens)
- Soluble cytochrome b562,5-hydroxytryptamine receptor 6 (63 kDa, Protein from Homo sapiens)
- Serotonin (176 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Serotonin 7 (5-HT7) receptor-Gs-Nb35 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7xtc
Q-score: 0.455
Huang S, Xu P, Shen DD, Simon IA, Mao C, Tan Y, Zhang H, Harpsoe K, Li H, Zhang Y, You C, Yu X, Jiang Y, Zhang Y, Gloriam DE, Xu HE
Mol Cell (2022) 82 pp. 2681-2695.e6 [ Pubmed: 35714614 DOI: doi:10.1016/j.molcel.2022.05.031 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Cholesterol (386 Da, Ligand)
- Soluble cytochrome b562,5-hydroxytryptamine receptor 7 (64 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Serotonin 7 (5-ht7) receptor-gs-nb35 complex (Complex from Homo sapiens)
- 3-(2-azanylethyl)-1h-indole-5-carboxamide (203 Da, Ligand)
Cryo-EM structure of the fenoldopam-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7x2c
Q-score: 0.465
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ DOI: doi:10.1038/s41467-022-30929-w Pubmed: 35676276 ]
- Nanobody35 (17 kDa, Protein from synthetic construct)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- The fenoldopam-bound d1r-minigs (Complex)
- (1r)-6-chloranyl-1-(4-hydroxyphenyl)-2,3,4,5-tetrahydro-1h-3-benzazepine-7,8-diol (305 Da, Ligand)
- Cryo-em structure of the fenoldopam-bound d1r-minigs complex (Complex from Homo sapiens)
- Nanobody35 (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Gbeta-1gama-2 (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the tavapadon-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7x2d
Q-score: 0.495
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ DOI: doi:10.1038/s41467-022-30929-w Pubmed: 35676276 ]
- Nanobody35 (17 kDa, Protein from synthetic construct)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cryo-em structure of the tavapadon-bound d1r-minigs complex (Complex from Homo sapiens)
- Nanobody35 (Complex)
- 1,5-dimethyl-6-[2-methyl-4-[3-(trifluoromethyl)pyridin-2-yl]oxy-phenyl]pyrimidine-2,4-dione (391 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Tavapadon-bound d1r-minigs (Complex)
Cryo-EM structure of the dopamine and LY3154207-bound D1 dopamine receptor and mini-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7x2f
Q-score: 0.471
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun (2022) 13 pp. 3186-3186 [ DOI: doi:10.1038/s41467-022-30929-w Pubmed: 35676276 ]
- Nanobody35 (17 kDa, Protein from synthetic construct)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Nanobody35 (Complex)
- 2-[2,6-bis(chloranyl)phenyl]-1-[(1s,3r)-3-(hydroxymethyl)-1-methyl-5-(3-methyl-3-oxidanyl-butyl)-3,4-dihydro-1h-isoquinolin-2-yl]ethanone (450 Da, Ligand)
- Dopamine-ly3154207-bound d1r-minigs (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Cryo-em structure of the dopamine-ly3154207-bound d1r-minigs complexthe (Complex from Homo sapiens)
Cryo-EM structure of the PGE2-bound EP2-Gs complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7cx2
Q-score: 0.536
Qu C, Mao C, Xiao P, Shen Q, Zhong YN, Yang F, Shen DD, Tao X, Zhang H, Yan X, Zhao RJ, He J, Guan Y, Zhang C, Hou G, Zhang PJ, Hou G, Li Z, Yu X, Chai RJ, Guan YF, Sun JP, Zhang Y
Sci Adv (2021) 7 [ Pubmed: 33811074 DOI: doi:10.1126/sciadv.abf1268 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Prostaglandin e2 receptor ep2 subtype (39 kDa, Protein from Homo sapiens)
- (z)-7-[(1r,2r,3r)-3-hydroxy-2-[(e,3s)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid (352 Da, Ligand)
- Ep2 (Complex from Homo sapiens)
- Pge2-bound ep2-gs complex (Complex)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Gs (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Nb35 (Complex from synthetic construct)