Cryo-EM structure of the amthamine-bound H2R-Gs complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8yut
Q-score: 0.529
Shen Q, Tang X, Wen X, Cheng S, Xiao P, Zang SK, Shen DD, Jiang L, Zheng Y, Zhang H, Xu H, Mao C, Zhang M, Hu W, Sun JP, Zhang Y, Chen Z
Adv Sci (2024) 11 pp. e2310120-e2310120 [ Pubmed: 38647423 DOI: doi:10.1002/advs.202310120 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Amthamine-bound h2r-gs complex (Complex from Homo sapiens)
- 5-(2-azanylethyl)-4-methyl-1,3-thiazol-2-amine (157 Da, Ligand)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Histamine h2 receptor (46 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the METH-TAAR1 complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8w87
Q-score: 0.567
Liu H, Zheng Y, Wang Y, Wang Y, He X, Xu P, Huang S, Yuan Q, Zhang X, Wang L, Jiang K, Chen H, Li Z, Liu W, Wang S, Xu HE, Xu F
Nature (2023) 624 pp. 663-671 [ Pubmed: 37935377 DOI: doi:10.1038/s41586-023-06775-1 ]
- Trace amine-associated receptor 1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Cryo-em structure of the meth-taar1 complex (Complex from Homo sapiens)
- (2s)-n-methyl-1-phenylpropan-2-amine (149 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Cryo-EM structure of the SEP363856-bound TAAR1-Gs complex
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8w88
Q-score: 0.59
Liu H, Zheng Y, Wang Y, Wang Y, He X, Xu P, Huang S, Yuan Q, Zhang X, Wang L, Jiang K, Chen H, Li Z, Liu W, Wang S, Xu HE, Xu F
Nature (2023) 624 pp. 663-671 [ Pubmed: 37935377 DOI: doi:10.1038/s41586-023-06775-1 ]
- Sep363856-bound taar1-gs complex (Complex from Homo sapiens)
- Trace amine-associated receptor 1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- 1-[(7~{s})-5,7-dihydro-4~{h}-thieno[2,3-c]pyran-7-yl]-~{n}-methyl-methanamine (183 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the PEA-bound TAAR1-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8w89
Q-score: 0.507
Liu H, Zheng Y, Wang Y, Wang Y, He X, Xu P, Huang S, Yuan Q, Zhang X, Wang L, Jiang K, Chen H, Li Z, Liu W, Wang S, Xu HE, Xu F
Nature (2023) 624 pp. 663-671 [ Pubmed: 37935377 DOI: doi:10.1038/s41586-023-06775-1 ]
- Trace amine-associated receptor 1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cryo-em structure of the pea-bound taar1-gs complex (Complex from Homo sapiens)
- 2-phenylethylamine (122 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
Cryo-EM structure of the RO5256390-TAAR1 complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8w8a
Q-score: 0.551
Liu H, Zheng Y, Wang Y, Wang Y, He X, Xu P, Huang S, Yuan Q, Zhang X, Wang L, Jiang K, Chen H, Li Z, Liu W, Wang S, Xu HE, Xu F
Nature (2023) 624 pp. 663-671 [ Pubmed: 37935377 DOI: doi:10.1038/s41586-023-06775-1 ]
- Trace amine-associated receptor 1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cryo-em structure of the ro5256390-taar1 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- (4s)-4-[(2s)-2-phenylbutyl]-1,3-oxazolidin-2-imine (218 Da, Ligand)
Cryo-EM structure of the GPR174-Gs complex bound to endogenous lysoPS
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 8kh5
Q-score: 0.514
Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S
Nat Commun (2023) 14 pp. 5901-5901 [ DOI: doi:10.1038/s41467-023-41654-3 Pubmed: 37737235 ]
- Gpr174 (Complex from Homo sapiens)
- Probable g-protein coupled receptor 174 (39 kDa, Protein from Homo sapiens)
- Nb35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- O-{hydroxy[((2r)-2-hydroxy-3-{[(1s)-1-hydroxypentadecyl]oxy}propyl)oxy]phosphoryl}-l-serine (485 Da, Ligand)
- Gs complex (Complex from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Cryo-em structure of the gpr174 and mini-gs complex with nb35 (Complex)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the GPR161-Gs complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8kh4
Q-score: 0.48
Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S
Nat Commun (2023) 14 pp. 5901-5901 [ DOI: doi:10.1038/s41467-023-41654-3 Pubmed: 37737235 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 161 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(olf) subunit alpha,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gpr161 and mini-gs complex with nb35 (Complex from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the DMCHA-bound mTAAR9-Gs complex
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 8itf
Q-score: 0.491
Guo L, Cheng J, Lian S, Liu Q, Lu Y, Zheng Y, Zhu K, Zhang M, Kong Y, Zhang C, Rong N, Zhuang Y, Fang G, Jiang J, Zhang T, Han X, Liu Z, Xia M, Liu S, Zhang L, Liberles SD, Yu X, Xu Y, Yang F, Li Q, Sun JP
Nature (2023) 618 pp. 193-200 [ Pubmed: 37225986 DOI: doi:10.1038/s41586-023-06106-4 ]
- ~{n},~{n}-dimethylcyclohexanamine (127 Da, Ligand)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Cryo-em structure of the dmcha-bound mtaar9-gs complex (Complex from Mus musculus)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Trace amine-associated receptor 9 (38 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (42 kDa, Protein from Homo sapiens)
TSHR-thyroid stimulating hormone-Gs-ML109 complex
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 7xw5
Q-score: 0.482
Duan J, Xu P, Luan X, Ji Y, He X, Song N, Yuan Q, Jin Y, Cheng X, Jiang H, Zheng J, Zhang S, Jiang Y, Xu HE
Nature (2022) 609 pp. 854-859 [ Pubmed: 35940204 DOI: doi:10.1038/s41586-022-05173-3 ]
- Glycoprotein hormones alpha chain (10 kDa, Protein from Homo sapiens)
- Thyrotropin subunit beta (13 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Thyrotropin receptor (79 kDa, Protein from Homo sapiens)
- ~{n}-[4-[[2-methoxy-5-[(2~{s})-5-oxidanyl-4-oxidanylidene-3-(phenylmethyl)-1,2-dihydroquinazolin-2-yl]phenyl]methoxy]phenyl]ethanamide (523 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Nanobody35 (14 kDa, Protein from Lama glama)
- Tshr-thyroid stimulating hormone-gs-ml109 complex (Complex from Homo)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
TSHR-Gs-M22 antibody-ML109 complex
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 7xw6
Q-score: 0.441
Duan J, Xu P, Luan X, Ji Y, He X, Song N, Yuan Q, Jin Y, Cheng X, Jiang H, Zheng J, Zhang S, Jiang Y, Xu HE
Nature (2022) 609 pp. 854-859 [ Pubmed: 35940204 DOI: doi:10.1038/s41586-022-05173-3 ]
- The complex was assembled by co-expression. (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Nanobody35 (14 kDa, Protein from Homo sapiens)
- M22 antibody light chain (11 kDa, Protein from Mus musculus)
- Palmitic acid (256 Da, Ligand)
- Thyrotropin receptor (79 kDa, Protein from Homo sapiens)
- ~{n}-[4-[[2-methoxy-5-[(2~{s})-5-oxidanyl-4-oxidanylidene-3-(phenylmethyl)-1,2-dihydroquinazolin-2-yl]phenyl]methoxy]phenyl]ethanamide (523 Da, Ligand)
- M22 antibody heavy chain (13 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)