Cryo-EM structure of human DICER-pre-miRNA in a dicing state
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 7xw2
Q-score: 0.444
Lee YY, Lee H, Kim H, Kim VN, Roh SH
Nature (2023) 615 pp. 331-338 [ DOI: doi:10.1038/s41586-023-05723-3 Pubmed: 36813958 ]
- Human dicer (Complex from Homo sapiens)
- Rna (73-mer) (23 kDa, RNA from Homo sapiens)
- Pre-mirna (Complex)
- Protein-rna complex of human dicer with pre-mirna (Complex)
- Calcium ion (40 Da, Ligand)
- Endoribonuclease dicer (218 kDa, Protein from Homo sapiens)
Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex IIa
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8dfv
Q-score: 0.447
Jouravleva K, Golovenko D, Demo G, Dutcher RC, Hall TMT, Zamore PD, Korostelev AA
Mol Cell (2022) 82 pp. 4049-4063.e6 [ Pubmed: 36182693 DOI: doi:10.1016/j.molcel.2022.09.002 ]
- Ternary complex of dicer-1 and loqs-pb binding pre-mirna (Complex from Drosophila melanogaster)
- Endoribonuclease dcr-1 (255 kDa, Protein from Drosophila melanogaster)
- Loquacious, isoform b (50 kDa, Protein from Drosophila melanogaster)
- Calcium ion (40 Da, Ligand)
- Rna (60-mer) (19 kDa, RNA from Drosophila melanogaster)
Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex IIb
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 8dg5
Q-score: 0.406
Jouravleva K, Golovenko D, Demo G, Dutcher RC, Hall TMT, Zamore PD, Korostelev AA
Mol Cell (2022) 82 pp. 4049-4063.e6 [ Pubmed: 36182693 DOI: doi:10.1016/j.molcel.2022.09.002 ]
- Rna (60-mer) (18 kDa, RNA from Drosophila melanogaster)
- Magnesium ion (24 Da, Ligand)
- Ternary complex of dicer-1 and loqs-pb binding pre-mirna (Complex from Drosophila melanogaster)
- Endoribonuclease dcr-1 (255 kDa, Protein from Drosophila melanogaster)
- Loquacious, isoform b (50 kDa, Protein from Drosophila melanogaster)
- Uridine-5'-monophosphate (324 Da, Ligand)
Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex III
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 8dg7
Q-score: 0.399
Jouravleva K, Golovenko D, Demo G, Dutcher RC, Hall TMT, Zamore PD, Korostelev AA
Mol Cell (2022) 82 pp. 4049-4063.e6 [ Pubmed: 36182693 DOI: doi:10.1016/j.molcel.2022.09.002 ]
- Magnesium ion (24 Da, Ligand)
- Ternary complex of dicer-1 and loqs-pb binding pre-mirna (Complex from Drosophila melanogaster)
- Endoribonuclease dcr-1 (255 kDa, Protein from Drosophila melanogaster)
- Loquacious, isoform b (50 kDa, Protein from Drosophila melanogaster)
- Rna (22-mer) (6 kDa, RNA from Drosophila melanogaster)
- Rna (38-mer) (11 kDa, RNA from Drosophila melanogaster)
- Uridine-5'-monophosphate (324 Da, Ligand)
Cryo-EM structure of Arabidopsis DCL1 in complex with pre-miRNA 166f
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 7ele
Q-score: 0.186
Wei X, Ke H, Wen A, Gao B, Shi J, Feng Y
Nat Plants (2021) 7 pp. 1389-1396 [ Pubmed: 34593993 DOI: doi:10.1038/s41477-021-01000-1 ]
- Binary complex of dcl1 in complex with pre-mirna 166f (Complex from Arabidopsis thaliana)
- Endoribonuclease dicer homolog 1 (213 kDa, Protein from Arabidopsis thaliana)
- Pre-mirna 166f (28 kDa, RNA from Arabidopsis thaliana)