Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 9b5c
Q-score: 0.614
Kochanczyk T, Hann ZS, Lux MC, Delos Reyes AMV, Ji C, Tan DS, Lima CD
Nature (2024) 633 pp. 216-223 [ DOI: doi:10.1038/s41586-024-07828-9 Pubmed: 39143218 ]
- Covalent e1-ub-e2 transthiolation intermediate mimic complex with second ub bound to e1 (doubly ub-loaded e1-ub-e2 complex) (Complex from Schizosaccharomyces pombe 972h-)
- 4-aminobutanenitrile (84 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ubiquitin-activating enzyme e1 1 (111 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Large ribosomal subunit protein el40b (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin-conjugating enzyme e2 4 (17 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 9b5d
Q-score: 0.552
Kochanczyk T, Hann ZS, Lux MC, Delos Reyes AMV, Ji C, Tan DS, Lima CD
Nature (2024) 633 pp. 216-223 [ DOI: doi:10.1038/s41586-024-07828-9 Pubmed: 39143218 ]
- Covalent e1-ub-e2 transthiolation intermediate mimic complex with second ub bound to e1 (doubly ub-loaded e1-ub-e2 complex) (Complex from Schizosaccharomyces pombe 972h-)
- 4-aminobutanenitrile (84 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ubiquitin-activating enzyme e1 1 (111 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Large ribosomal subunit protein el40b (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin-conjugating enzyme e2 4 (17 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 9b5e
Q-score: 0.558
Kochanczyk T, Hann ZS, Lux MC, Delos Reyes AMV, Ji C, Tan DS, Lima CD
Nature (2024) 633 pp. 216-223 [ DOI: doi:10.1038/s41586-024-07828-9 Pubmed: 39143218 ]
- Covalent e1-ub-e2 transthiolation intermediate mimic complex with second ub bound to e1 (doubly ub-loaded e1-ub-e2 complex) (Complex from Schizosaccharomyces pombe 972h-)
- 4-aminobutanenitrile (84 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ubiquitin-activating enzyme e1 1 (111 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Large ribosomal subunit protein el40b (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin-conjugating enzyme e2 4 (17 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 9b5g
Q-score: 0.601
Kochanczyk T, Hann ZS, Lux MC, Delos Reyes AMV, Ji C, Tan DS, Lima CD
Nature (2024) 633 pp. 216-223 [ DOI: doi:10.1038/s41586-024-07828-9 Pubmed: 39143218 ]
- Covalent e1-ub-e2 transthiolation intermediate mimic complex with second ub bound to e1 (doubly ub-loaded e1-ub-e2 complex) (Complex from Schizosaccharomyces pombe 972h-)
- 4-aminobutanenitrile (84 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ubiquitin-activating enzyme e1 1 (111 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Large ribosomal subunit protein el40b (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin-conjugating enzyme e2 4 (17 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 9b5h
Q-score: 0.601
Kochanczyk T, Hann ZS, Lux MC, Delos Reyes AMV, Ji C, Tan DS, Lima CD
Nature (2024) 633 pp. 216-223 [ DOI: doi:10.1038/s41586-024-07828-9 Pubmed: 39143218 ]
- Pyrophosphate 2- (175 Da, Ligand)
- Covalent e1-ub-e2 transthiolation intermediate mimic complex with second ub bound to e1 (doubly ub-loaded e1-ub-e2 complex) (Complex from Schizosaccharomyces pombe 972h-)
- Adenosine monophosphate (347 Da, Ligand)
- 4-aminobutanenitrile (84 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ubiquitin-activating enzyme e1 1 (111 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Large ribosomal subunit protein el40b (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin-conjugating enzyme e2 4 (17 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 9b5j
Q-score: 0.559
Kochanczyk T, Hann ZS, Lux MC, Delos Reyes AMV, Ji C, Tan DS, Lima CD
Nature (2024) 633 pp. 216-223 [ DOI: doi:10.1038/s41586-024-07828-9 Pubmed: 39143218 ]
- Covalent e1-ub-e2 transthiolation intermediate mimic complex with second ub bound to e1 (doubly ub-loaded e1-ub-e2 complex) (Complex from Schizosaccharomyces pombe 972h-)
- Adenosine monophosphate (347 Da, Ligand)
- 4-aminobutanenitrile (84 Da, Ligand)
- Ubiquitin-activating enzyme e1 1 (111 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Large ribosomal subunit protein el40b (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin-conjugating enzyme e2 4 (17 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 9b5k
Q-score: 0.483
Kochanczyk T, Hann ZS, Lux MC, Delos Reyes AMV, Ji C, Tan DS, Lima CD
Nature (2024) 633 pp. 216-223 [ DOI: doi:10.1038/s41586-024-07828-9 Pubmed: 39143218 ]
- Covalent e1-ub-e2 transthiolation intermediate mimic complex with second ub bound to e1 (doubly ub-loaded e1-ub-e2 complex) (Complex from Schizosaccharomyces pombe 972h-)
- 4-aminobutanenitrile (84 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ubiquitin-activating enzyme e1 1 (111 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Large ribosomal subunit protein el40b (8 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin-conjugating enzyme e2 4 (17 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Ubiquitin (8 kDa, Protein from Schizosaccharomyces pombe 972h-)