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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

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perform basic research in molecular biology

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offer vital services to scientists in the member states

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scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"protein E" AND citation_journal_name:"MOL CELL" AND author_name:"Puglisi"

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EMD-17329

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48S late-stage initiation complex with m6A mRNA

Release date: March 20, 2024
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 8p03
Q-score: 0.509

Guca E, Alarcon R, Palo MZ, Santos L, Alonso-Gil S, Davyt M, de Lima LHF, Boissier F, Das S, Zagrovic B, Puglisi JD, Hashem Y, Ignatova Z

Mol Cell (2024) 84 pp. 584-595.e6 [ DOI: doi:10.1016/j.molcel.2023.12.028 Pubmed: 38244546 ]

Sample:

40s ribosomal protein s25 (13 kDa, Protein from Oryctolagus cuniculus)
60s ribosomal protein l41 (3 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein es17 (15 kDa, Protein from Oryctolagus cuniculus)
Ribosomal protein es1 (24 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s29 (6 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein us9 (15 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s23 (15 kDa, Protein from Oryctolagus cuniculus)
48s late-stage initiation complex with m6a-modified mrna (Complex from Oryctolagus cuniculus)
Ribosomal protein s15a (14 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein es10 (11 kDa, Protein from Oryctolagus cuniculus)
Eukaryotic translation initiation factor 2 subunit 1 (32 kDa, Protein from Oryctolagus cuniculus)
Ribosomal protein s5 (21 kDa, Protein from Oryctolagus cuniculus)
Initiator methionylated trna (24 kDa, RNA from Oryctolagus cuniculus)
Ribosomal protein rack1 (34 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s8 (23 kDa, Protein from Oryctolagus cuniculus)
Eukaryotic translation initiation factor 4c (12 kDa, Protein from Oryctolagus cuniculus)
M6a-methylated mrna (2 kDa, RNA from Oryctolagus cuniculus)
Magnesium ion (24 Da, Ligand)
40s ribosomal protein s6 (27 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s27 (9 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein es19 (15 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein us11 (16 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s30 (14 kDa, Protein from Oryctolagus cuniculus)
Ribosomal protein us15 (17 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s2 (29 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein us13 (17 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s21 (9 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein sa (23 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s12 (14 kDa, Protein from Oryctolagus cuniculus)
18s ribosomal rna (601 kDa, RNA from Oryctolagus cuniculus)
Ribosomal protein s3 (25 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s24 (15 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s9 (22 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein us19 (17 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s11 (18 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s4 (29 kDa, Protein from Oryctolagus cuniculus)
Ribosomal protein es31 (8 kDa, Protein from Oryctolagus cuniculus)
Ribosomal protein es7 (21 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s28 (7 kDa, Protein from Oryctolagus cuniculus)
Atp binding cassette subfamily e member 1 (66 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s26 (12 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein us10 (13 kDa, Protein from Oryctolagus cuniculus)

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EMD-17330

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48S late-stage initiation complex with non methylated mRNA

Release date: March 20, 2024
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8p09
Q-score: 0.494

Guca E, Alarcon R, Palo MZ, Santos L, Alonso-Gil S, Davyt M, de Lima LHF, Boissier F, Das S, Zagrovic B, Puglisi JD, Hashem Y, Ignatova Z

Mol Cell (2024) 84 pp. 584-595.e6 [ DOI: doi:10.1016/j.molcel.2023.12.028 Pubmed: 38244546 ]

Sample:

Mrna (2 kDa, RNA from Oryctolagus cuniculus)
40s ribosomal protein s25 (13 kDa, Protein from Oryctolagus cuniculus)
60s ribosomal protein l41 (3 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein es17 (15 kDa, Protein from Oryctolagus cuniculus)
Ribosomal protein es1 (24 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s29 (6 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein us9 (15 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s23 (15 kDa, Protein from Oryctolagus cuniculus)
Ribosomal protein s15a (14 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein es10 (11 kDa, Protein from Oryctolagus cuniculus)
Eukaryotic translation initiation factor 2 subunit 1 (32 kDa, Protein from Oryctolagus cuniculus)
Ribosomal protein s5 (21 kDa, Protein from Oryctolagus cuniculus)
Initiator methionylated trna (24 kDa, RNA from Oryctolagus cuniculus)
Ribosomal protein rack1 (34 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s8 (23 kDa, Protein from Oryctolagus cuniculus)
Eukaryotic translation initiation factor 4c (12 kDa, Protein from Oryctolagus cuniculus)
Magnesium ion (24 Da, Ligand)
40s ribosomal protein s6 (27 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s27 (9 kDa, Protein from Oryctolagus cuniculus)
48s late-stage initiation complex with non methylated mrna (Complex from Oryctolagus cuniculus)
40s ribosomal protein es19 (15 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein us11 (16 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s30 (14 kDa, Protein from Oryctolagus cuniculus)
Ribosomal protein us15 (17 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s2 (29 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein us13 (17 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s21 (9 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein sa (23 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s12 (14 kDa, Protein from Oryctolagus cuniculus)
18s ribosomal rna (601 kDa, RNA from Oryctolagus cuniculus)
Ribosomal protein s3 (25 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s24 (15 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s9 (22 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein us19 (17 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s11 (18 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s4 (29 kDa, Protein from Oryctolagus cuniculus)
Ribosomal protein es31 (8 kDa, Protein from Oryctolagus cuniculus)
Ribosomal protein es7 (21 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s28 (7 kDa, Protein from Oryctolagus cuniculus)
Atp binding cassette subfamily e member 1 (66 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein s26 (12 kDa, Protein from Oryctolagus cuniculus)
40s ribosomal protein us10 (13 kDa, Protein from Oryctolagus cuniculus)

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EMD-17804

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Bat-Hp-CoV Nsp1 and eIF1 bound to the human 40S small ribosomal subunit

Release date: Oct. 18, 2023
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8ppk
Q-score: 0.489

Schubert K, Karousis ED, Ban I, Lapointe CP, Leibundgut M, Baumlin E, Kummerant E, Scaiola A, Schonhut T, Ziegelmuller J, Puglisi JD, Muhlemann O, Ban N

Mol Cell (2023) 83 pp. 3546-3557.e8 [ Pubmed: 37802027 DOI: doi:10.1016/j.molcel.2023.09.002 ]

Sample:

40s ribosomal protein s7 (22 kDa, Protein from Homo sapiens)
Ubiquitin-40s ribosomal protein s27a (18 kDa, Protein from Homo sapiens)
40s ribosomal protein s29 (6 kDa, Protein from Homo sapiens)
40s ribosomal protein s3 (26 kDa, Protein from Homo sapiens)
40s ribosomal protein s3a (30 kDa, Protein from Homo sapiens)
40s ribosomal protein s15 (17 kDa, Protein from Homo sapiens)
40s ribosomal protein s20 (13 kDa, Protein from Homo sapiens)
Small ribosomal subunit protein es30 (14 kDa, Protein from Homo sapiens)
60s ribosomal protein l41 (3 kDa, Protein from Homo sapiens)
40s ribosomal protein s21 (9 kDa, Protein from Homo sapiens)
40s ribosomal protein s23 (15 kDa, Protein from Homo sapiens)
40s ribosomal protein s24 (15 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
40s ribosomal protein s16 (16 kDa, Protein from Homo sapiens)
40s ribosomal protein s26 (13 kDa, Protein from Homo sapiens)
40s ribosomal protein sa (32 kDa, Protein from Homo sapiens)
Nsp1 (21 kDa, Protein from Bat Hp-betacoronavirus/Zhejiang2013)
40s ribosomal protein s9 (22 kDa, Protein from Homo sapiens)
40s ribosomal protein s17 (15 kDa, Protein from Homo sapiens)
40s ribosomal protein s5 (22 kDa, Protein from Homo sapiens)
40s ribosomal protein s4, x isoform (29 kDa, Protein from Homo sapiens)
40s ribosomal protein s15a (14 kDa, Protein from Homo sapiens)
Unknown atom or ion (Ligand)
Zinc ion (65 Da, Ligand)
40s ribosomal protein s11 (18 kDa, Protein from Homo sapiens)
Receptor of activated protein c kinase 1 (35 kDa, Protein from Homo sapiens)
40s ribosomal protein s6 (28 kDa, Protein from Homo sapiens)
40s ribosomal protein s28 (7 kDa, Protein from Homo sapiens)
40s ribosomal protein s10 (18 kDa, Protein from Homo sapiens)
40s ribosomal protein s2 (31 kDa, Protein from Homo sapiens)
40s ribosomal protein s13 (17 kDa, Protein from Homo sapiens)
40s ribosomal protein s25 (13 kDa, Protein from Homo sapiens)
40s ribosomal protein s27 (9 kDa, Protein from Homo sapiens)
40s ribosomal protein s12 (14 kDa, Protein from Homo sapiens)
Bat-hp-cov nsp1 - eif1 - 40s complex (Complex from Homo sapiens)
Eukaryotic translation initiation factor 1 (12 kDa, Protein from Homo sapiens)
40s ribosomal protein s8 (24 kDa, Protein from Homo sapiens)
18s rrna (603 kDa, RNA from Homo sapiens)
Small ribosomal subunit protein us13 (17 kDa, Protein from Homo sapiens)
Small ribosomal subunit protein es19 (16 kDa, Protein from Homo sapiens)
40s ribosomal protein s14 (16 kDa, Protein from Homo sapiens)

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EMD-17805

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MERS-CoV Nsp1 bound to the human 43S pre-initiation complex

Release date: Oct. 18, 2023
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 8ppl
Q-score: 0.427

Schubert K, Karousis ED, Ban I, Lapointe CP, Leibundgut M, Baumlin E, Kummerant E, Scaiola A, Schonhut T, Ziegelmuller J, Puglisi JD, Muhlemann O, Ban N

Mol Cell (2023) 83 pp. 3546-3557.e8 [ Pubmed: 37802027 DOI: doi:10.1016/j.molcel.2023.09.002 ]

Sample:

Eukaryotic translation initiation factor 3 subunit d (64 kDa, Protein from Homo sapiens)
40s ribosomal protein s14 (16 kDa, Protein from Homo sapiens)
Mers-cov nsp1 - 43s pre-initiation complex (Complex from Homo sapiens)
40s ribosomal protein s7 (22 kDa, Protein from Homo sapiens)
Eukaryotic translation initiation factor 3 subunit f (37 kDa, Protein from Homo sapiens)
Ubiquitin-40s ribosomal protein s27a (18 kDa, Protein from Homo sapiens)
40s ribosomal protein s29 (6 kDa, Protein from Homo sapiens)
Methionine (149 Da, Ligand)
40s ribosomal protein s3 (26 kDa, Protein from Homo sapiens)
40s ribosomal protein s3a (30 kDa, Protein from Homo sapiens)
40s ribosomal protein s15 (17 kDa, Protein from Homo sapiens)
Eukaryotic translation initiation factor 3 subunit h (39 kDa, Protein from Homo sapiens)
40s ribosomal protein s20 (13 kDa, Protein from Homo sapiens)
60s ribosomal protein l41 (3 kDa, Protein from Homo sapiens)
40s ribosomal protein s21 (9 kDa, Protein from Homo sapiens)
40s ribosomal protein s23 (15 kDa, Protein from Homo sapiens)
40s ribosomal protein s24 (15 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
Met-trnai(met) (24 kDa, RNA from Homo sapiens)
40s ribosomal protein s16 (16 kDa, Protein from Homo sapiens)
Eukaryotic translation initiation factor 3 subunit k (25 kDa, Protein from Homo sapiens)
Eukaryotic translation initiation factor 3 subunit m (42 kDa, Protein from Homo sapiens)
40s ribosomal protein s26 (13 kDa, Protein from Homo sapiens)
Eukaryotic translation initiation factor 3 subunit a (166 kDa, Protein from Homo sapiens)
40s ribosomal protein sa (32 kDa, Protein from Homo sapiens)
Eukaryotic translation initiation factor 2 subunit 2 (38 kDa, Protein from Homo sapiens)
Eukaryotic translation initiation factor 2 subunit 3 (51 kDa, Protein from Homo sapiens)
Eukaryotic translation initiation factor 3 subunit e (52 kDa, Protein from Homo sapiens)
Ubiquitin-like fubi-ribosomal protein es30 fusion protein (14 kDa, Protein from Homo sapiens)
40s ribosomal protein s17 (15 kDa, Protein from Homo sapiens)
Eukaryotic translation initiation factor 2 subunit 1 (36 kDa, Protein from Homo sapiens)
40s ribosomal protein s5 (22 kDa, Protein from Homo sapiens)
40s ribosomal protein s4, x isoform (29 kDa, Protein from Homo sapiens)
40s ribosomal protein s15a (14 kDa, Protein from Homo sapiens)
Unknown atom or ion (Ligand)
Zinc ion (65 Da, Ligand)
Eukaryotic translation initiation factor 1a, x-chromosomal (16 kDa, Protein from Homo sapiens)
40s ribosomal protein s11 (18 kDa, Protein from Homo sapiens)
Receptor of activated protein c kinase 1 (35 kDa, Protein from Homo sapiens)
40s ribosomal protein s6 (28 kDa, Protein from Homo sapiens)
40s ribosomal protein s28 (7 kDa, Protein from Homo sapiens)
40s ribosomal protein s2 (31 kDa, Protein from Homo sapiens)
40s ribosomal protein s10 (18 kDa, Protein from Homo sapiens)
Eukaryotic translation initiation factor 3 subunit g (35 kDa, Protein from Homo sapiens)
40s ribosomal protein s13 (17 kDa, Protein from Homo sapiens)
Eukaryotic translation initiation factor 3 subunit c (105 kDa, Protein from Homo sapiens)
Water (18 Da, Ligand)
Eukaryotic translation initiation factor 3 subunit l (66 kDa, Protein from Homo sapiens)
40s ribosomal protein s25 (13 kDa, Protein from Homo sapiens)
Host translation inhibitor nsp1 (24 kDa, Protein from Middle East respiratory syndrome-related coronavirus)
Guanosine-5'-triphosphate (523 Da, Ligand)
40s ribosomal protein s27 (9 kDa, Protein from Homo sapiens)
40s ribosomal protein s12 (14 kDa, Protein from Homo sapiens)
Eukaryotic translation initiation factor 1 (12 kDa, Protein from Homo sapiens)
40s ribosomal protein s8 (24 kDa, Protein from Homo sapiens)
18s rrna (603 kDa, RNA from Homo sapiens)
Small ribosomal subunit protein us13 (17 kDa, Protein from Homo sapiens)
Small ribosomal subunit protein es19 (16 kDa, Protein from Homo sapiens)
40s ribosomal protein s9 (22 kDa, Protein from Homo sapiens)

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