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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"protein L27" AND author_name:"Gao N" AND xref_links:"go" AND author_name:"M"

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EMD-30170

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Cryo-EM structure of pre-60S ribosome from Saccharomyces cerevisiae rpl4delta63-87 strain at 3.12 Angstroms resolution(state R1)

Release date: Oct. 28, 2020
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 7bt6
Q-score: 0.533

Wilson DM, Li Y, LaPeruta A, Gamalinda M, Gao N, Woolford Jr JL

Nat Commun (2020) 11 pp. 5111-5111 [ Pubmed: 33037216 DOI: doi:10.1038/s41467-020-18878-8 ]

Sample:

60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Eukaryotic pre-60s ribosomal subunits from saccharomyces cerevisiae rpl4delta63-87 strain(stater1) (Complex from Saccharomyces cerevisiae S288C)
60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rdn5-2 rrna (38 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Magnesium ion (24 Da, Ligand)
60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Regulator of ribosome biosynthesis (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome biogenesis protein rpf2 (39 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleolar gtp-binding protein 2 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome assembly protein 4 (57 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Zinc ion (65 Da, Ligand)
60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rdn5.8-1 rrna (50 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rdn25-1 rrna (1 MDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Guanosine-5'-triphosphate (523 Da, Ligand)
60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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EMD-30174

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Cryo-EM structure of pre-60S ribosome from Saccharomyces cerevisiae rpl4delta63-87 strain at 3.22 Angstroms resolution(state R2)

Release date: Oct. 28, 2020
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 7btb
Q-score: 0.51

Wilson DM, Li Y, LaPeruta A, Gamalinda M, Gao N, Woolford Jr JL

Nat Commun (2020) 11 pp. 5111-5111 [ Pubmed: 33037216 DOI: doi:10.1038/s41467-020-18878-8 ]

Sample:

60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Eukaryotic pre-60s ribosomal subunits from saccharomyces cerevisiae rpl4delta63-87 strain(stater2) (Complex from Saccharomyces cerevisiae S288C)
60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rdn5.8-1 rrna (50 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome biogenesis protein nop53 (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Magnesium ion (24 Da, Ligand)
60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Its2-1 miscrna (74 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Regulator of ribosome biosynthesis (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome biogenesis protein rpf2 (39 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleolar gtp-binding protein 2 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rdn5-2 rrna (38 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome assembly protein 4 (57 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rdn25-1 rrna (1 MDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Zinc ion (65 Da, Ligand)
60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Guanosine-5'-triphosphate (523 Da, Ligand)
60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

[Show more]

EMD-0948

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Cryo-EM structure of a pre-60S ribosomal subunit - state C

Release date: Aug. 26, 2020
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 6lqm
Q-score: 0.555

Liang X, Zuo MQ, Zhang Y, Li N, Ma C, Dong MQ, Gao N

Nat Commun (2020) 11 pp. 3542-3542 [ Pubmed: 32669547 DOI: doi:10.1038/s41467-020-17237-x ]

Sample:

60s ribosomal protein l5 (34 kDa, Protein from Human)
60s ribosomal export protein nmd3 (61 kDa, Protein from Homo sapiens)
60s ribosomal protein l28 (15 kDa, Protein from Human)
60s ribosomal protein l21 (18 kDa, Protein from Human)
60s ribosomal protein l6 (32 kDa, Protein from Human)
60s ribosomal protein l4 (47 kDa, Protein from Human)
60s ribosomal protein l7 (29 kDa, Protein from Human)
60s ribosomal protein l37 (11 kDa, Protein from Human)
60s ribosomal protein l18a (20 kDa, Protein from Human)
60s ribosomal protein l17 (21 kDa, Protein from Human)
60s ribosomal protein l26 (17 kDa, Protein from Human)
60s ribosomal protein l38 (8 kDa, Protein from Human)
60s ribosomal protein l18 (21 kDa, Protein from Human)
60s ribosomal protein l11 (20 kDa, Protein from Human)
60s ribosomal protein l24 (17 kDa, Protein from Human)
60s ribosomal protein l3 (46 kDa, Protein from Human)
60s ribosomal protein l8 (28 kDa, Protein from Human)
Magnesium ion (24 Da, Ligand)
Cryo-em structure of a human pre-60s ribosomal subunit state - c (Complex from Homo sapiens)
Zinc finger protein 622 (54 kDa, Protein from Human)
28s rrna (1 MDa, RNA from Human)
60s ribosomal protein l22 (14 kDa, Protein from Human)
60s ribosomal protein l29 (17 kDa, Protein from Human)
60s ribosomal protein l10-like (24 kDa, Protein from Human)
60s ribosomal protein l36 (12 kDa, Protein from Human)
5.8s rrna (50 kDa, RNA from Human)
60s ribosomal protein l7a (30 kDa, Protein from Human)
5s rrna (38 kDa, RNA from Human)
60s ribosomal protein l19 (23 kDa, Protein from Human)
60s ribosomal protein l13 (24 kDa, Protein from Human)
Eukaryotic translation initiation factor 6 (26 kDa, Protein from Human)
60s ribosomal protein l10a (24 kDa, Protein from Human)
60s ribosomal protein l34 (13 kDa, Protein from Human)
60s ribosomal protein l23 (14 kDa, Protein from Human)
60s ribosomal protein l32 (15 kDa, Protein from Human)
60s ribosomal protein l31 (14 kDa, Protein from Human)
60s ribosomal protein l27a (16 kDa, Protein from Human)
Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Human)
60s ribosomal protein l36a (12 kDa, Protein from Human)
60s ribosomal protein l37a (10 kDa, Protein from Human)
60s ribosomal protein l35a (12 kDa, Protein from Human)
60s ribosomal protein l13a (23 kDa, Protein from Human)
60s ribosomal protein l27 (15 kDa, Protein from Human)
60s ribosomal protein l23a (17 kDa, Protein from Human)
60s ribosomal protein l39 (6 kDa, Protein from Human)
Cryo-em structure of a human pre-60s ribosomal subunit state - c (Complex)
60s ribosomal protein l9 (21 kDa, Protein from Human)
60s ribosomal protein l30 (12 kDa, Protein from Human)
60s ribosomal protein l35 (14 kDa, Protein from Human)
60s ribosomal protein l15 (24 kDa, Protein from Human)
60s ribosomal export protein nmd3 (Complex from Homo sapiens)
60s ribosomal protein l14 (23 kDa, Protein from Human)

[Show more]

EMD-0963

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Cryo-EM structure of a pre-60S ribosomal subunit - state B

Release date: Aug. 26, 2020
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 6lsr
Q-score: 0.532

Liang X, Zuo MQ, Zhang Y, Li N, Ma C, Dong MQ, Gao N

Nat Commun (2020) 11 pp. 3542-3542 [ Pubmed: 32669547 DOI: doi:10.1038/s41467-020-17237-x ]

Sample:

60s ribosomal protein l5 (34 kDa, Protein from Human)
60s ribosomal export protein nmd3 (61 kDa, Protein from Homo sapiens)
60s ribosomal protein l28 (15 kDa, Protein from Human)
60s ribosomal protein l21 (18 kDa, Protein from Human)
Proliferation-associated protein 2g4 (43 kDa, Protein from Human)
60s ribosomal protein l6 (32 kDa, Protein from Human)
60s ribosomal protein l4 (47 kDa, Protein from Human)
Large subunit gtpase 1 homolog (75 kDa, Protein from Human)
60s ribosomal protein l7 (29 kDa, Protein from Human)
60s ribosomal protein l37 (11 kDa, Protein from Human)
60s ribosomal protein l18a (20 kDa, Protein from Human)
60s ribosomal protein l17 (21 kDa, Protein from Human)
60s ribosomal protein l26 (17 kDa, Protein from Human)
60s ribosomal protein l38 (8 kDa, Protein from Human)
60s ribosomal protein l18 (21 kDa, Protein from Human)
60s ribosomal protein l11 (20 kDa, Protein from Human)
60s ribosomal protein l24 (17 kDa, Protein from Human)
60s ribosomal protein l3 (46 kDa, Protein from Human)
60s ribosomal protein l8 (28 kDa, Protein from Human)
Magnesium ion (24 Da, Ligand)
Zinc finger protein 622 (54 kDa, Protein from Human)
Cryo-em structure of a human pre-60s ribosomal subunit state - b (Complex from Homo sapiens)
28s rrna (1 MDa, RNA from Human)
60s ribosomal protein l22 (14 kDa, Protein from Human)
60s ribosomal protein l29 (17 kDa, Protein from Human)
60s ribosomal protein l36 (12 kDa, Protein from Human)
5.8s rrna (50 kDa, RNA from Human)
60s ribosomal protein l7a (30 kDa, Protein from Human)
5s rrna (38 kDa, RNA from Human)
60s ribosomal protein l19 (23 kDa, Protein from Human)
60s ribosomal protein l13 (24 kDa, Protein from Human)
Eukaryotic translation initiation factor 6 (26 kDa, Protein from Human)
60s ribosomal protein l10a (24 kDa, Protein from Human)
60s ribosomal protein l34 (13 kDa, Protein from Human)
60s ribosomal protein l23 (14 kDa, Protein from Human)
60s ribosomal protein l32 (15 kDa, Protein from Human)
60s ribosomal protein l31 (14 kDa, Protein from Human)
60s ribosomal protein l27a (16 kDa, Protein from Human)
Cryo-em structure of a human pre-60s ribosomal subunit state - b (Complex)
60s ribosomal protein l36a (12 kDa, Protein from Human)
60s ribosomal protein l37a (10 kDa, Protein from Human)
60s ribosomal protein l35a (12 kDa, Protein from Human)
Water (18 Da, Ligand)
60s ribosomal protein l13a (23 kDa, Protein from Human)
60s ribosomal protein l27 (15 kDa, Protein from Human)
60s ribosomal protein l23a (17 kDa, Protein from Human)
60s ribosomal protein l39 (6 kDa, Protein from Human)
60s ribosomal protein l9 (21 kDa, Protein from Human)
60s ribosomal protein l30 (12 kDa, Protein from Human)
60s ribosomal protein l35 (14 kDa, Protein from Human)
60s ribosomal protein l15 (24 kDa, Protein from Human)
60s ribosomal export protein nmd3 (Complex from Homo sapiens)
60s ribosomal protein l14 (23 kDa, Protein from Human)

[Show more]

EMD-0964

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Cryo-EM structure of a pre-60S ribosomal subunit - state preA

Release date: Aug. 26, 2020
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 6lss
Q-score: 0.515

Liang X, Zuo MQ, Zhang Y, Li N, Ma C, Dong MQ, Gao N

Nat Commun (2020) 11 pp. 3542-3542 [ Pubmed: 32669547 DOI: doi:10.1038/s41467-020-17237-x ]

Sample:

60s ribosomal protein l5 (34 kDa, Protein from Human)
60s ribosomal protein l28 (15 kDa, Protein from Human)
60s ribosomal protein l21 (18 kDa, Protein from Human)
60s ribosomal protein l6 (32 kDa, Protein from Human)
60s ribosomal protein l4 (47 kDa, Protein from Human)
60s ribosomal protein l7 (29 kDa, Protein from Human)
60s ribosomal protein l37 (11 kDa, Protein from Human)
Zinc finger protein 593 (15 kDa, Protein from Human)
60s ribosomal protein l18a (20 kDa, Protein from Human)
Translation machinery-associated protein 16 (23 kDa, Protein from Human)
60s ribosomal protein l17 (21 kDa, Protein from Human)
60s ribosomal protein l26 (17 kDa, Protein from Human)
60s ribosomal protein l38 (8 kDa, Protein from Human)
60s ribosomal protein l18 (21 kDa, Protein from Human)
60s ribosomal protein l11 (20 kDa, Protein from Human)
60s ribosomal protein l8 (28 kDa, Protein from Human)
60s ribosomal protein l3 (46 kDa, Protein from Human)
Nucleolar gtp-binding protein 2 (83 kDa, Protein from Human)
Magnesium ion (24 Da, Ligand)
Protein llp homolog (15 kDa, Protein from Human)
28s rrna (1 MDa, RNA from Human)
60s ribosomal protein l22 (14 kDa, Protein from Human)
60s ribosomal protein l29 (17 kDa, Protein from Human)
Probable ribosome biogenesis protein rlp24 (19 kDa, Protein from Human)
60s ribosomal protein l36 (12 kDa, Protein from Human)
Cryo-em structure of a human pre-60s ribosomal subunit state - prea (Complex from Homo sapiens)
60s ribosomal protein l7a (30 kDa, Protein from Human)
5s rrna (38 kDa, RNA from Human)
60s ribosomal protein l19 (23 kDa, Protein from Human)
60s ribosomal protein l13 (24 kDa, Protein from Human)
Eukaryotic translation initiation factor 6 (26 kDa, Protein from Human)
60s ribosomal protein l32 (15 kDa, Protein from Human)
60s ribosomal protein l34 (13 kDa, Protein from Human)
60s ribosomal protein l23 (14 kDa, Protein from Human)
60s ribosomal protein l31 (14 kDa, Protein from Human)
60s ribosomal protein l27a (16 kDa, Protein from Human)
60s ribosomal protein l36a (12 kDa, Protein from Human)
60s ribosomal protein l37a (10 kDa, Protein from Human)
28s rrna (50 kDa, RNA from Human)
60s ribosomal protein l35a (12 kDa, Protein from Human)
Water (18 Da, Ligand)
60s ribosomal protein l13a (23 kDa, Protein from Human)
60s ribosomal protein l27 (15 kDa, Protein from Human)
60s ribosomal protein l23a (17 kDa, Protein from Human)
60s ribosomal protein l39 (6 kDa, Protein from Human)
60s ribosomal protein l9 (21 kDa, Protein from Human)
60s ribosomal protein l30 (12 kDa, Protein from Human)
60s ribosomal protein l35 (14 kDa, Protein from Human)
60s ribosomal protein l15 (24 kDa, Protein from Human)
Nucleolar gtp-binding protein 1 (74 kDa, Protein from Human)
60s ribosomal protein l14 (23 kDa, Protein from Human)

[Show more]

EMD-0978

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Cryo-EM structure of a human pre-60S ribosomal subunit - state A

Release date: Aug. 26, 2020
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 6lu8
Q-score: 0.54

Liang X, Zuo MQ, Zhang Y, Li N, Ma C, Dong MQ, Gao N

Nat Commun (2020) 11 pp. 3542-3542 [ Pubmed: 32669547 DOI: doi:10.1038/s41467-020-17237-x ]

Sample:

60s ribosomal protein l5 (34 kDa, Protein from Human)
60s ribosomal protein l28 (15 kDa, Protein from Human)
60s ribosomal protein l21 (18 kDa, Protein from Human)
60s ribosomal protein l6 (32 kDa, Protein from Human)
60s ribosomal protein l4 (47 kDa, Protein from Human)
60s ribosomal protein l7 (29 kDa, Protein from Human)
60s ribosomal protein l37 (11 kDa, Protein from Human)
Zinc finger protein 593 (15 kDa, Protein from Human)
60s ribosomal protein l18a (20 kDa, Protein from Human)
Translation machinery-associated protein 16 (23 kDa, Protein from Human)
60s ribosomal protein l17 (21 kDa, Protein from Human)
60s ribosomal protein l26 (17 kDa, Protein from Human)
60s ribosomal protein l38 (8 kDa, Protein from Human)
60s ribosomal protein l18 (21 kDa, Protein from Human)
60s ribosomal export protein nmd3 (57 kDa, Protein from Homo sapiens)
60s ribosomal protein l11 (20 kDa, Protein from Human)
60s ribosomal protein l3 (46 kDa, Protein from Human)
Nucleolar gtp-binding protein 2 (83 kDa, Protein from Human)
60s ribosomal protein l8 (28 kDa, Protein from Human)
Magnesium ion (24 Da, Ligand)
Protein llp homolog (15 kDa, Protein from Human)
28s rrna (1 MDa, RNA from Human)
60s ribosomal protein l22 (14 kDa, Protein from Human)
60s ribosomal protein l29 (17 kDa, Protein from Human)
Probable ribosome biogenesis protein rlp24 (19 kDa, Protein from Human)
60s ribosomal protein l36 (12 kDa, Protein from Human)
60s ribosomal protein l7a (30 kDa, Protein from Human)
5s rrna (38 kDa, RNA from Human)
60s ribosomal protein l19 (23 kDa, Protein from Human)
60s ribosomal protein l13 (24 kDa, Protein from Human)
Eukaryotic translation initiation factor 6 (26 kDa, Protein from Human)
60s ribosomal protein l10a (24 kDa, Protein from Human)
60s ribosomal protein l34 (13 kDa, Protein from Human)
60s ribosomal protein l23 (14 kDa, Protein from Human)
60s ribosomal protein l32 (15 kDa, Protein from Human)
60s ribosomal protein l31 (14 kDa, Protein from Human)
60s ribosomal protein l27a (16 kDa, Protein from Human)
60s ribosomal protein l36a (12 kDa, Protein from Human)
60s ribosomal protein l37a (10 kDa, Protein from Human)
60s ribosomal protein l35a (12 kDa, Protein from Human)
Water (18 Da, Ligand)
60s ribosomal protein l13a (23 kDa, Protein from Human)
60s ribosomal protein l27 (15 kDa, Protein from Human)
Cryo-em structure of a human pre-60s ribosomal subunit state - a (Complex)
5.8s rrna (50 kDa, RNA from Human)
60s ribosomal protein l23a (17 kDa, Protein from Human)
60s ribosomal protein l39 (6 kDa, Protein from Human)
60s ribosomal protein l9 (21 kDa, Protein from Human)
60s ribosomal protein l30 (12 kDa, Protein from Human)
60s ribosomal protein l35 (14 kDa, Protein from Human)
60s ribosomal protein l15 (24 kDa, Protein from Human)
60s ribosomal export protein nmd3 (Complex from Homo sapiens)
Cryo-em structure of a human pre-60s ribosomal subunit state - a (Complex from Homo sapiens)
Nucleolar gtp-binding protein 1 (74 kDa, Protein from Human)
60s ribosomal protein l14 (23 kDa, Protein from Human)

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EMDB [6]

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