Structure of human serotonin transporter bound to small molecule '8090 in lipid nanodisc and NaCl
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7txt
Q-score: 0.549
Singh I, Seth A, Billesbolle CB, Braz J, Rodriguiz RM, Roy K, Bekele B, Craik V, Huang XP, Boytsov D, Pogorelov VM, Lak P, O'Donnell H, Sandtner W, Irwin JJ, Roth BL, Basbaum AI, Wetsel WC, Manglik A, Shoichet BK, Rudnick G
Cell (2023) 186 pp. 2160-2175.e17 [ Pubmed: 37137306 DOI: doi:10.1016/j.cell.2023.04.010 ]
- Fab15b8 light chain (Complex from Mus musculus)
- Sodium-dependent serotonin transporter (61 kDa, Protein from Homo sapiens)
- Fab15b8 heavy chain (Complex from Mus musculus)
- Human serotonin transporter in complex with fab15b8 bound to '8090 in nacl (Complex)
- 1-[4-(4-fluorophenyl)-1,3-thiazol-2-yl]piperazine (263 Da, Ligand)
- Sodium-dependent serotonin transporter (Complex from Homo sapiens)
- 15b8 fab light chain (11 kDa, Protein from Mus musculus)
- 15b8 fab heavy chain (13 kDa, Protein from Mus musculus)
apo SERT reconstituted in lipid nanodisc in KCl
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7li6
Q-score: 0.436
Sci Adv (2021) 7 pp. eabl3857-eabl3857 [ Pubmed: 34851672 DOI: doi:10.1126/sciadv.abl3857 ]
- Hexadecane (226 Da, Ligand)
- Sodium-dependent serotonin transporter (60 kDa, Protein from Homo sapiens)
- Pentane (72 Da, Ligand)
- Dodecane (170 Da, Ligand)
- Variable domain of 15b8 antibody fab heavy chain (12 kDa, Protein from Mus musculus)
- Heptane (100 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- Variable domain of 15b8 antibody fab light chain (11 kDa, Protein from Mus musculus)
- Decane (142 Da, Ligand)
- Apo state of human serotonin transporter in complex with 15b8 fab in kcl (Complex from Homo sapiens)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7li7
Q-score: 0.364
Sci Adv (2021) 7 pp. eabl3857-eabl3857 [ Pubmed: 34851672 DOI: doi:10.1126/sciadv.abl3857 ]
- Pentane (72 Da, Ligand)
- Dodecane (170 Da, Ligand)
- Variable domain of 15b8 antibody fab heavy chain (12 kDa, Protein from Mus musculus)
- Sodium-dependent serotonin transporter (60 kDa, Protein from Homo sapiens)
- Heptane (100 Da, Ligand)
- Variable domain of 15b8 antibody fab light chain (11 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- Decane (142 Da, Ligand)
- Apo human serotonin transporter in complex with 15b8 fab in nacl (Complex from Homo sapiens)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7li8
Q-score: 0.376
Sci Adv (2021) 7 pp. eabl3857-eabl3857 [ Pubmed: 34851672 DOI: doi:10.1126/sciadv.abl3857 ]
- Hexadecane (226 Da, Ligand)
- Sodium-dependent serotonin transporter (60 kDa, Protein from Homo sapiens)
- Pentane (72 Da, Ligand)
- Dodecane (170 Da, Ligand)
- Variable domain of 15b8 antibody fab heavy chain (12 kDa, Protein from Mus musculus)
- Heptane (100 Da, Ligand)
- Variable domain of 15b8 antibody fab light chain (11 kDa, Protein from Mus musculus)
- Decane (142 Da, Ligand)
- Apo human serotonin transporter in complex with 15b8 fab in nacl (Complex from Homo sapiens)
5-HT bound serotonin transporter reconstituted in lipid nanodisc in KCl
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7li9
Q-score: 0.38
Sci Adv (2021) 7 pp. eabl3857-eabl3857 [ Pubmed: 34851672 DOI: doi:10.1126/sciadv.abl3857 ]
- Sodium-dependent serotonin transporter (60 kDa, Protein from Homo sapiens)
- Serotonin (176 Da, Ligand)
- Pentane (72 Da, Ligand)
- Dodecane (170 Da, Ligand)
- Variable domain of 15b8 antibody fab heavy chain (12 kDa, Protein from Mus musculus)
- Heptane (100 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- Variable domain of 15b8 antibody fab light chain (11 kDa, Protein from Mus musculus)
- Decane (142 Da, Ligand)
- Human serotonin transporter in complex with 15b8 fab in kcl in presence of serotonin (Complex from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7lia
Q-score: 0.498
Sci Adv (2021) 7 pp. eabl3857-eabl3857 [ Pubmed: 34851672 DOI: doi:10.1126/sciadv.abl3857 ]
- Sodium-dependent serotonin transporter (60 kDa, Protein from Homo sapiens)
- Serotonin (176 Da, Ligand)
- Pentane (72 Da, Ligand)
- Human serotonin transporter in complex with 15b8 fab in presence of serotonin in nacl (Complex from Homo sapiens)
- Variable domain of 15b8 antibody fab heavy chain (12 kDa, Protein from Mus musculus)
- Heptane (100 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- Variable domain of 15b8 antibody fab light chain (11 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- Decane (142 Da, Ligand)
- Cholesterol (386 Da, Ligand)
5-HT bound serotonin transporter reconstituted in lipid nanodisc in NaCl in occluded conformation
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7mgw
Q-score: 0.362
Sci Adv (2021) 7 pp. eabl3857-eabl3857 [ Pubmed: 34851672 DOI: doi:10.1126/sciadv.abl3857 ]
- Serotonin (176 Da, Ligand)
- Pentane (72 Da, Ligand)
- Dodecane (170 Da, Ligand)
- Variable domain of 15b8 antibody fab heavy chain (12 kDa, Protein from Mus musculus)
- Sodium-dependent serotonin transporter (60 kDa, Protein from Homo sapiens)
- Heptane (100 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Decane (142 Da, Ligand)
- Apo human serotonin transporter in complex with 15b8 fab in nacl (Complex from Homo sapiens)
- Variable domain of 15b8 antiboty fab light chain (11 kDa, Protein from Mus musculus)
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 7lwd
Q-score: 0.466
Plenge P, Yang D, Salomon K, Laursen L, Kalenderoglou IE, Newman AH, Gouaux E, Coleman JA, Loland CJ
Nat Commun (2021) 12 pp. 5063-5063 [ Pubmed: 34417466 DOI: doi:10.1038/s41467-021-25363-3 ]
- Heavy chain antibody fragment (12 kDa, Protein from Mus musculus)
- 8b6 antibody (Complex from Mus musculus)
- Serotonin transporter (Complex from Homo sapiens)
- Light chain antibody fragment (11 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 5-{4-[4-(5-cyano-1h-indol-3-yl)butyl]piperazin-1-yl}-1-benzofuran-2-carboxamide (441 Da, Ligand)
- Serotonin transporter 8b6 fab complex (105 kDa, Complex)
- 3-(5h-dibenzo[b,f]azepin-5-yl)-n,n-dimethylpropan-1-amine (280 Da, Ligand)
- Sodium-dependent serotonin transporter (61 kDa, Protein from Homo sapiens)
Cryo-EM structure of the wild-type human serotonin transporter complexed with paroxetine and 8B6 Fab
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6vrh
Q-score: 0.454
Coleman JA, Navratna V, Antermite D, Yang D, Bull JA, Gouaux E
eLife (2020) 9 [ Pubmed: 32618269 DOI: doi:10.7554/eLife.56427 ]
- 8b6 antibody (Complex from Mus musculus)
- Serotonin transporter (Complex from Homo sapiens)
- 8b6 antibody, heavy chain (23 kDa, Protein from Mus musculus)
- Serotonin transporter 8b6 fab complex (105 kDa, Complex)
- Paroxetine (329 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- 8b6 antibody, light chain (23 kDa, Protein from Mus musculus)
- Sodium-dependent serotonin transporter (70 kDa, Protein from Homo sapiens)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6vrk
Q-score: 0.336
Coleman JA, Navratna V, Antermite D, Yang D, Bull JA, Gouaux E
eLife (2020) 9 [ Pubmed: 32618269 DOI: doi:10.7554/eLife.56427 ]
- 8b6 antibody (Complex from Mus musculus)
- Serotonin transporter (Complex from Homo sapiens)
- 8b6 antibody, heavy chain (23 kDa, Protein from Mus musculus)
- Serotonin transporter 8b6 fab complex (105 kDa, Complex)
- Br-paroxetine (390 Da, Ligand)
- 8b6 antibody, light chain (23 kDa, Protein from Mus musculus)
- Sodium-dependent serotonin transporter (70 kDa, Protein from Homo sapiens)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)