Cryo-EM structure of the octreotide-bound SSTR2-miniGo-scFv16 complex
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 7y24
Q-score: 0.442
Nat Chem Biol (2023) 19 pp. 133-140 [ DOI: doi:10.1038/s41589-022-01130-3 Pubmed: 36138141 ]
- Complex of sstr2-minigo-scfv16 bound with octreotide (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- G(o) subunit alpha (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (25 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Single fab chain (svfv16) (26 kDa, Protein from Homo sapiens)
- Svfv16, octreotide (Complex from Homo sapiens)
- Octreotide (1 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 2 (Complex)
- G(i)/g(s)/g(t) subunit beta-1, g(i)/g(s)/g(o) subunit gamma-2 (Complex)
- Somatostatin receptor type 2 (36 kDa, Protein from Homo sapiens)
Cryo-EM structure of the octreotide-bound SSTR2-miniGq-scFv16 complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7y26
Q-score: 0.468
Nat Chem Biol (2023) 19 pp. 133-140 [ DOI: doi:10.1038/s41589-022-01130-3 Pubmed: 36138141 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Engineered guanine nucleotide-binding protein g(q) subunit alpha (27 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 2 (37 kDa, Protein from Homo sapiens)
- Single fab chain (svfv16) (27 kDa, Protein from Homo sapiens)
- Octreotide (Complex)
- G(i)/g(s)/g(t) subunit beta-1, g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Octreotide (1 kDa, Protein from Homo sapiens)
- G(q) subunit alpha, sstr2, svfv16 (Complex)
- Complex of sstr2-minigq-scfv16 bound with octreotide (Complex from Homo sapiens)
Cryo-EM structure of the SST-14-bound SSTR2-miniGq-scFv16 complex
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 7y27
Q-score: 0.464
Nat Chem Biol (2023) 19 pp. 133-140 [ DOI: doi:10.1038/s41589-022-01130-3 Pubmed: 36138141 ]
- Engineered guanine nucleotide-binding protein g(q) subunit alpha (27 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 2 (37 kDa, Protein from Homo sapiens)
- Single fab chain (svfv16) (27 kDa, Protein from Homo sapiens)
- Complex of sstr2-minigq-scfv16 bound with sst-14 (Complex from Homo sapiens)
- Sst-14 (Complex)
- G(i)/g(s)/g(t) subunit beta-1, g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Somatostatin-14 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- G(q) subunit alpha, sstr2, svfv16 (Complex)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7xmr
Q-score: 0.41
Zhao W, Han S, Qiu N, Feng W, Lu M, Zhang W, Wang M, Zhou Q, Chen S, Xu W, Du J, Chu X, Yi C, Dai A, Hu L, Shen MY, Sun Y, Zhang Q, Ma Y, Zhong W, Yang D, Wang MW, Wu B, Zhao Q
Cell Res (2022) 32 pp. 761-772 [ Pubmed: 35739238 DOI: doi:10.1038/s41422-022-00679-x ]
- Somatostatin-14 (1 kDa, Protein from Homo sapiens)
- Complex structure of the somatostatin receptor 2 (sstr2) in complex with gi1 and its endogeneous peptide ligand sst-14 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 2 (45 kDa, Protein from Homo sapiens)
Cryo-EM structure of the SS-14-bound human SSTR2-Gi1 complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7wic
Q-score: 0.509
Chen LN, Wang WW, Dong YJ, Shen DD, Guo J, Yu X, Qin J, Ji SY, Zhang H, Shen Q, He Q, Yang B, Zhang Y, Li Q, Mao C
Cell Res (2022) 32 pp. 785-788 [ DOI: doi:10.1038/s41422-022-00669-z Pubmed: 35578016 ]
- Cryo-em structure of the ss-14-bound sstr2-gi1 complex (180 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- G protein (Complex from Homo sapiens)
- Somatostatin receptor type 2 (41 kDa, Protein from Homo sapiens)
- Somatostatin-14 (1 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 2 (Complex from Homo sapiens)
- Antibody (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Single fab chain (svfv16) (28 kDa, Protein from synthetic construct)
Cryo-EM structure of the L-054,264-bound human SSTR2-Gi1 complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7wig
Q-score: 0.527
Chen LN, Wang WW, Dong YJ, Shen DD, Guo J, Yu X, Qin J, Ji SY, Zhang H, Shen Q, He Q, Yang B, Zhang Y, Li Q, Mao C
Cell Res (2022) 32 pp. 785-788 [ DOI: doi:10.1038/s41422-022-00669-z Pubmed: 35578016 ]
- ~{n}-[(2~{r})-1-[[(1~{r},3~{s})-3-(aminomethyl)cyclohexyl]methylamino]-3-(1~{h}-indol-3-yl)-1-oxidanylidene-propan-2-yl]spiro[indene-1,4'-piperidine]-1'-carboxamide (539 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- G protein (Complex from Homo sapiens)
- Somatostatin receptor type 2 (41 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 2 (Complex from Homo sapiens)
- Cryo-em structure of the l-054,264-bound sstr2-gi1 complex (180 kDa, Complex)
- Antibody (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Single fab chain (svfv16) (28 kDa, Protein from synthetic construct)