Cryo-EM structure of the SST-14-bound SSTR2-miniGq-scFv16 complex
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 7y27
Q-score: 0.464
Nat Chem Biol (2023) 19 pp. 133-140 [ DOI: doi:10.1038/s41589-022-01130-3 Pubmed: 36138141 ]
- G(i)/g(s)/g(t) subunit beta-1, g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Single fab chain (svfv16) (27 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 2 (37 kDa, Protein from Homo sapiens)
- Somatostatin-14 (1 kDa, Protein from Homo sapiens)
- G(q) subunit alpha, sstr2, svfv16 (Complex)
- Engineered guanine nucleotide-binding protein g(q) subunit alpha (27 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Sst-14 (Complex)
- Complex of sstr2-minigq-scfv16 bound with sst-14 (Complex from Homo sapiens)
Structure of somatostatin receptor 2 bound with SST14.
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 7xat
Q-score: 0.545
Bo Q, Yang F, Li Y, Meng X, Zhang H, Zhou Y, Ling S, Sun D, Lv P, Liu L, Shi P, Tian C
Cell Discov (2022) 8 pp. 47-47 [ DOI: doi:10.1038/s41421-022-00405-2 Pubmed: 35595746 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Bos taurus)
- Somatostatin receptor type 2,largebit (63 kDa, Protein from synthetic construct)
- Somatostatin-14 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Scfv16 (32 kDa, Protein from Rattus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- The endogenous ligand sst14-bound somatostatin receptor 2 and gi heterotrimer complex. (Complex from Homo sapiens)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7xmr
Q-score: 0.41
Zhao W, Han S, Qiu N, Feng W, Lu M, Zhang W, Wang M, Zhou Q, Chen S, Xu W, Du J, Chu X, Yi C, Dai A, Hu L, Shen MY, Sun Y, Zhang Q, Ma Y, Zhong W, Yang D, Wang MW, Wu B, Zhao Q
Cell Res (2022) 32 pp. 761-772 [ DOI: doi:10.1038/s41422-022-00679-x Pubmed: 35739238 ]
- Complex structure of the somatostatin receptor 2 (sstr2) in complex with gi1 and its endogeneous peptide ligand sst-14 (Complex from Homo sapiens)
- Somatostatin-14 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 2 (45 kDa, Protein from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7xms
Q-score: 0.408
Zhao W, Han S, Qiu N, Feng W, Lu M, Zhang W, Wang M, Zhou Q, Chen S, Xu W, Du J, Chu X, Yi C, Dai A, Hu L, Shen MY, Sun Y, Zhang Q, Ma Y, Zhong W, Yang D, Wang MW, Wu B, Zhao Q
Cell Res (2022) 32 pp. 761-772 [ Pubmed: 35739238 DOI: doi:10.1038/s41422-022-00679-x ]
- Signle chain viable fragment of antibody (31 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Complex structure of somatostatin receptor 4 (sstr4) in complex with gi and its endogeneous ligand sst-14 (Complex from Homo sapiens)
- Somatostatin-14 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 4 (40 kDa, Protein from Homo sapiens)
Resolution: 3.72 Å
EM Method: Single-particle
Fitted PDBs: 7wj5
Q-score: 0.485
Heo Y, Yoon E, Jeon YE, Yun JH, Ishimoto N, Woo H, Park SY, Song JJ, Lee W
eLife (2022) 11 [ DOI: doi:10.7554/eLife.76823 Pubmed: 35446253 ]
- Somatostatin-14 (sst-14) (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,somatostatin receptor type 2 (58 kDa, Protein from Homo sapiens)
- Human sstr2 and sst-14 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Gai1 antibody (scfv16) (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of the SS-14-bound human SSTR2-Gi1 complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7wic
Q-score: 0.509
Chen LN, Wang WW, Dong YJ, Shen DD, Guo J, Yu X, Qin J, Ji SY, Zhang H, Shen Q, He Q, Yang B, Zhang Y, Li Q, Mao C
Cell Res (2022) 32 pp. 785-788 [ DOI: doi:10.1038/s41422-022-00669-z Pubmed: 35578016 ]
- G protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Single fab chain (svfv16) (28 kDa, Protein from synthetic construct)
- Somatostatin-14 (1 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 2 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Antibody (Complex from synthetic construct)
- Somatostatin receptor type 2 (Complex from Homo sapiens)
- Cryo-em structure of the ss-14-bound sstr2-gi1 complex (180 kDa, Complex)
CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3.
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7t10
Q-score: 0.615
Robertson MJ, Meyerowitz JG, Panova O, Borrelli K, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 210-217 [ DOI: doi:10.1038/s41594-022-00727-5 Pubmed: 35210615 ]
- Somatostatin-14 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Somatostatin receptor 2 in complex with somatostatin-14 and gi3. (Complex)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-3 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Somatostatin receptor type 2,kappa-type opioid receptor (45 kDa, Protein from Homo sapiens)