N5-methyl-H4MPT:CoM methyltransferase -coenzyme M complex + CoM
Aziz I, Kayastha K, Kaltwasser S, Vonck J, Welsch S, Murohy BJ, Kahnt J, Wu D, Wagner T, Shima S, Ermler U
To Be Published
- Tetrahydromethanopterin s-methyltransferase subunit b (10 kDa, Protein from Methanothermobacter marburgensis)
- 1-thioethanesulfonic acid (142 Da, Ligand)
- Tetrahydromethanopterin s-methyltransferase subunit e (31 kDa, Protein from Methanothermobacter marburgensis)
- Tetrahydromethanopterin s-methyltransferase subunit a 1 (25 kDa, Protein from Methanothermobacter marburgensis)
- Sodium ion (22 Da, Ligand)
- Methyl-h4mpt:com methyltransferase (430 MDa, Complex from Methanothermobacter marburgensis)
- Tetrahydromethanopterin s-methyltransferase subunit c (27 kDa, Protein from Methanothermobacter marburgensis)
- Tetrahydromethanopterin s-methyltransferase subunit f (7 kDa, Protein from Methanothermobacter marburgensis)
- Tetrahydromethanopterin s-methyltransferase subunit d (22 kDa, Protein from Methanothermobacter marburgensis)
- Magnesium ion (24 Da, Ligand)
- Tetrahydromethanopterin s-methyltransferase subunit g (9 kDa, Protein from Methanothermobacter marburgensis)
- Water (18 Da, Ligand)
- [(2~{s},7~{r},11~{r},15~{s},19~{s},22~{s},26~{s},30~{r},34~{r},39~{s},43~{r},47~{r},51~{s},55~{s},58~{s},62~{s},66~{r},70~{r})-39-[[(2~{r},3~{r},4~{s},5~{s},6~{r})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxymethyl]-7,11,15,19,22,26,30,34,43,47,51,55,58,62,66,70-hexadecamethyl-1,4,37,40-tetraoxacyclodoheptacont-2-yl]methyl [(2~{r},3~{s},5~{r},6~{r})-2,3,4,5,6-pentakis(oxidanyl)cyclohexyl] hydrogen phosphate (1 kDa, Ligand)
Resolution: 2.08 Å
EM Method: Single-particle
Fitted PDBs: 8q3v
Q-score: 0.686
Aziz I, Kayastha K, Kaltwasser S, Vonck J, Welsch S, Murphy BJ, Kahnt J, Wu D, Wagner T, Shima S, Ermler U
PNAS (2024) 121 pp. e2315568121-e2315568121 [ Pubmed: 38530900 DOI: doi:10.1073/pnas.2315568121 ]
- Tetrahydromethanopterin s-methyltransferase subunit b (10 kDa, Protein from Methanothermobacter marburgensis)
- Tetrahydromethanopterin s-methyltransferase subunit e (31 kDa, Protein from Methanothermobacter marburgensis)
- Tetrahydromethanopterin s-methyltransferase subunit a 1 (25 kDa, Protein from Methanothermobacter marburgensis)
- Sodium ion (22 Da, Ligand)
- Methyl-h4mpt:com methyltransferase (430 MDa, Complex from Methanothermobacter marburgensis)
- Tetrahydromethanopterin s-methyltransferase subunit c (27 kDa, Protein from Methanothermobacter marburgensis)
- Tetrahydromethanopterin s-methyltransferase subunit f (7 kDa, Protein from Methanothermobacter marburgensis)
- Tetrahydromethanopterin s-methyltransferase subunit d (22 kDa, Protein from Methanothermobacter marburgensis)
- Magnesium ion (24 Da, Ligand)
- Tetrahydromethanopterin s-methyltransferase subunit g (9 kDa, Protein from Methanothermobacter marburgensis)
- Water (18 Da, Ligand)
- [(2~{s},7~{r},11~{r},15~{s},19~{s},22~{s},26~{s},30~{r},34~{r},39~{s},43~{r},47~{r},51~{s},55~{s},58~{s},62~{s},66~{r},70~{r})-39-[[(2~{r},3~{r},4~{s},5~{s},6~{r})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxymethyl]-7,11,15,19,22,26,30,34,43,47,51,55,58,62,66,70-hexadecamethyl-1,4,37,40-tetraoxacyclodoheptacont-2-yl]methyl [(2~{r},3~{s},5~{r},6~{r})-2,3,4,5,6-pentakis(oxidanyl)cyclohexyl] hydrogen phosphate (1 kDa, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7bkb
Q-score: 0.348
Watanabe T, Pfeil-Gardiner O, Kahnt J, Koch J, Shima S, Murphy BJ
Science (2021) 373 pp. 1151-1156 [ Pubmed: 34516836 DOI: doi:10.1126/science.abg5550 ]
- Formylmethanofuran dehydrogenase (28 kDa, Protein from Methanospirillum hungatei JF-1)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cob--com heterodisulfide reductase iron-sulfur subunit a (72 kDa, Protein from Methanospirillum hungatei JF-1)
- Non-cubane [4fe-4s]-cluster (351 Da, Ligand)
- Formate dehydrogenase, beta subunit (f420) (45 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit d (15 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit a (63 kDa, Protein from Methanospirillum hungatei JF-1)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Formate dehydrogenase (75 kDa, Protein from Methanospirillum hungatei JF-1)
- Cob--com heterodisulfide reductase subunit b (32 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit g (16 kDa, Protein from Methanospirillum hungatei JF-1)
- 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide (740 Da, Ligand)
- Molybdenum atom (95 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dimeric formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex (948 kDa, Complex from Methanospirillum hungatei JF-1)
- F420-non-reducing hydrogenase subunit d (15 kDa, Protein from Methanospirillum hungatei JF-1)
- Cob--com heterodisulfide reductase subunit c (21 kDa, Protein from Methanospirillum hungatei JF-1)
- Iron/sulfur cluster (351 Da, Ligand)
- Formylmethanofuran dehydrogenase, subunit f (42 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit b (49 kDa, Protein from Methanospirillum hungatei JF-1)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7bkc
Q-score: 0.569
Watanabe T, Pfeil-Gardiner O, Kahnt J, Koch J, Shima S, Murphy BJ
Science (2021) 373 pp. 1151-1156 [ Pubmed: 34516836 DOI: doi:10.1126/science.abg5550 ]
- Formylmethanofuran dehydrogenase (28 kDa, Protein from Methanospirillum hungatei JF-1)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cob--com heterodisulfide reductase iron-sulfur subunit a (72 kDa, Protein from Methanospirillum hungatei JF-1)
- Non-cubane [4fe-4s]-cluster (351 Da, Ligand)
- Formate dehydrogenase, beta subunit (f420) (45 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit d (15 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit a (63 kDa, Protein from Methanospirillum hungatei JF-1)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Formate dehydrogenase (75 kDa, Protein from Methanospirillum hungatei JF-1)
- Cob--com heterodisulfide reductase subunit b (32 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit g (16 kDa, Protein from Methanospirillum hungatei JF-1)
- 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide (740 Da, Ligand)
- Molybdenum atom (95 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dimeric formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex (948 kDa, Complex from Methanospirillum hungatei JF-1)
- F420-non-reducing hydrogenase subunit d (15 kDa, Protein from Methanospirillum hungatei JF-1)
- Cob--com heterodisulfide reductase subunit c (21 kDa, Protein from Methanospirillum hungatei JF-1)
- Iron/sulfur cluster (351 Da, Ligand)
- Formylmethanofuran dehydrogenase, subunit f (42 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit b (49 kDa, Protein from Methanospirillum hungatei JF-1)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7bkd
Q-score: 0.597
Watanabe T, Pfeil-Gardiner O, Kahnt J, Koch J, Shima S, Murphy BJ
Science (2021) 373 pp. 1151-1156 [ Pubmed: 34516836 DOI: doi:10.1126/science.abg5550 ]
- Non-cubane [4fe-4s]-cluster (351 Da, Ligand)
- Formate dehydrogenase (75 kDa, Protein from Methanospirillum hungatei JF-1)
- Dimeric formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex (948 kDa, Complex from Methanospirillum hungatei JF-1)
- Cob--com heterodisulfide reductase subunit b (32 kDa, Protein from Methanospirillum hungatei JF-1)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cob--com heterodisulfide reductase iron-sulfur subunit a (72 kDa, Protein from Methanospirillum hungatei JF-1)
- F420-non-reducing hydrogenase subunit d (15 kDa, Protein from Methanospirillum hungatei JF-1)
- Cob--com heterodisulfide reductase subunit c (21 kDa, Protein from Methanospirillum hungatei JF-1)
- Formate dehydrogenase, beta subunit (f420) (45 kDa, Protein from Methanospirillum hungatei JF-1)
- Iron/sulfur cluster (351 Da, Ligand)
- Flavin-adenine dinucleotide (785 Da, Ligand)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7bke
Q-score: 0.618
Watanabe T, Pfeil-Gardiner O, Kahnt J, Koch J, Shima S, Murphy BJ
Science (2021) 373 pp. 1151-1156 [ Pubmed: 34516836 DOI: doi:10.1126/science.abg5550 ]
- Non-cubane [4fe-4s]-cluster (351 Da, Ligand)
- Formate dehydrogenase (75 kDa, Protein from Methanospirillum hungatei JF-1)
- Dimeric formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex (948 kDa, Complex from Methanospirillum hungatei JF-1)
- Cob--com heterodisulfide reductase subunit b (32 kDa, Protein from Methanospirillum hungatei JF-1)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cob--com heterodisulfide reductase iron-sulfur subunit a (72 kDa, Protein from Methanospirillum hungatei JF-1)
- F420-non-reducing hydrogenase subunit d (15 kDa, Protein from Methanospirillum hungatei JF-1)
- Cob--com heterodisulfide reductase subunit c (21 kDa, Protein from Methanospirillum hungatei JF-1)
- Formate dehydrogenase, beta subunit (f420) (45 kDa, Protein from Methanospirillum hungatei JF-1)
- Iron/sulfur cluster (351 Da, Ligand)
- Flavin-adenine dinucleotide (785 Da, Ligand)